Germacrene-A synthase explained

The enzyme germacrene-A synthase (EC 4.2.3.23) catalyzes the chemical reaction

(2E,6E)-farnesyl diphosphate

(+)-(R)-gemacrene A + diphosphate

This enzyme belongs to the family of lyases, specifically those carbon-oxygen lyases acting on phosphates. The systematic name of this enzyme class is (2E,6E)-farnesyl-diphosphate diphosphate-lyase [(+)-(''R'')-germacrene-A-forming]. Other names in common use include germacrene A synthase, (+)-germacrene A synthase, (+)-(10R)-germacrene A synthase, GAS, 2-trans,6-trans-farnesyl-diphosphate diphosphate-lyase, (germacrene-A-forming).

References

• Wallaart TE . 2002 . Isolation and Characterization of Two Germacrene A Synthase cDNA Clones from Chicory . Plant Physiol. . 129 . 134 - 44 . 12011345 . 10.1104/pp.001024 . Kodde . J . Verstappen . FW . Altug . IG . De Kraker . JW . Wallaart . TE . 1 . 155878 .
• Beale MH . 2002 . (+)-(10R)-Germacrene A synthase from goldenrod, Solidago canadensis cDNA isolation, bacterial expression and functional analysis . . 60 . 691 - 702 . 12127586 . 10.1016/S0031-9422(02)00165-6 . Phillips . AL . Gittings . S . Lewis . MJ . Hooper . AM . Pickett . JA . Beale . MH . 7 .
• de Kraker JW, Franssen MC, de Groot A, Konig WA, Bouwmeester HJ . 1998 . (+)-Germacrene A Biosynthesis : The Committed Step in the Biosynthesis of Bitter Sesquiterpene Lactones in Chicory . Plant Physiol. . 117 . 1381 - 92 . 9701594 . 10.1104/pp.117.4.1381 . 4 . 34902 .
• Calvert MJ, Ashton PR, Allemann RK . 2002 . Germacrene A is a product of the aristolochene synthase-mediated conversion of farnesylpyrophosphate to aristolochene . J. Am. Chem. Soc. . 124 . 11636 - 41 . 12296728 . 10.1021/ja020762p . 39 .
• Chang YJ, Jin J, Nam HY, Kim SU . 2005 . Point mutation of (+)-germacrene A synthase from Ixeris dentata . Biotechnol. Lett. . 27 . 285 - 8 . 15834787 . 10.1007/s10529-005-0681-9 . 5 .