Condensation domain explained

In molecular biology, the condensation domain is a protein domain found in many multi-domain enzymes which synthesise peptide antibiotics. This domain catalyses a condensation reaction to form peptide bonds in non-ribosomal peptide biosynthesis. It is usually found to the carboxy side of a phosphopantetheine binding domain (pp-binding). It has been shown that mutations in the HHXXXDG sequence motif in this domain abolish activity suggesting this is part of the active site.^[1]

Notes and References

1. Stachelhaus T, Mootz HD, Bergendahl V, Marahiel MA . Peptide bond formation in nonribosomal peptide biosynthesis. Catalytic role of the condensation domain . J. Biol. Chem. . 273 . 35 . 22773–81 . August 1998 . 9712910 . 10.1074/jbc.273.35.22773. free .