The Zee Cine Award Best Actor in a Negative Role is chosen by a jury organized by Zee Entertainment Enterprises, and the winner is announced at the ceremony. It awards the actor or actress in the role of an antagonist.
Kajol was the first woman to win this award.
| 2 | Ashutosh Rana | |
| 2 | Bobby Deol |
The winners are listed below:-
| Year | Winner | Film |
|---|---|---|
| 1998 | ||
| 1999 | Dushman | |
| 2000 | Sangharsh | |
| 2001 | Jungle | |
| 2002 | Kasoor | |
| 2003 | Deewangee | |
| 2004 | Footpath | |
| 2005 | Dhoom | |
| 2006 | Apaharan | |
| 2007 | Omkara | |
| 2008 | Om Shanti Om | |
| 2009 | Not Held | |
| 2010 | Not Held | |
| 2011 | Udaan | |
| 2012 | Singham | |
| 2013 | Agneepath | |
| 2014 | Goliyon Ki Raasleela Ram-Leela | |
| 2015 | Not Held | |
| 2016 | Badlapur | |
| 2017 | Neerja | |
| 2018 | Secret Superstar | |
| 2019 | Andhadhun | |
| 2020 | Commando 3 War | |
| 2021 | Not Held | |
| 2022 | Not Held | |
| 2023 | Love Hostel | |
| 2024 | Animal | |