| Villaines-en-Duesmois | |
| Commune Status: | Commune |
| Image Coat Of Arms: | Blason de la ville de Villaines-en-Duesmois (21).svg |
| Arrondissement: | Montbard |
| Canton: | Châtillon-sur-Seine |
| Insee: | 21685 |
| Postal Code: | 21450 |
| Mayor: | Claude Fontaine[1] |
| Term: | 2020 - 2026 |
| Intercommunality: | Pays Châtillonnais |
| Coordinates: | 47.6831°N 4.5267°W |
| Elevation M: | 315 |
| Elevation Min M: | 306 |
| Elevation Max M: | 402 |
| Area Km2: | 33.95 |
Villaines-en-Duesmois is a commune in the Côte-d'Or department in eastern France.