UBE2M explained

NEDD8-conjugating enzyme Ubc12 is a protein that in humans is encoded by the UBE2M gene.^{[1] [2]}

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin.^[2]

Interactions

UBE2M has been shown to interact with NEDD8,^[3] PRKAR1A^[4] and UBA3.^[5]

Further reading

• Gong L, Yeh ET . Identification of the activating and conjugating enzymes of the NEDD8 conjugation pathway . J. Biol. Chem. . 274 . 17 . 12036–42 . 1999 . 10207026 . 10.1074/jbc.274.17.12036 . free .
• Chen Y, McPhie DL, Hirschberg J, Neve RL . The amyloid precursor protein-binding protein APP-BP1 drives the cell cycle through the S-M checkpoint and causes apoptosis in neurons . J. Biol. Chem. . 275 . 12 . 8929–35 . 2000 . 10722740 . 10.1074/jbc.275.12.8929 . free .
• Wada H, Yeh ET, Kamitani T . A dominant-negative UBC12 mutant sequesters NEDD8 and inhibits NEDD8 conjugation in vivo . J. Biol. Chem. . 275 . 22 . 17008–15 . 2000 . 10828074 . 10.1074/jbc.275.22.17008 . free .
• Strausberg RL . Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences . Proc. Natl. Acad. Sci. U.S.A. . 99 . 26 . 16899–903 . 2003 . 12477932 . 10.1073/pnas.242603899 . 139241 . vanc. Feingold EA . Grouse LH . 3 . Derge . JG . Klausner . RD . Collins . FS . Wagner . L . Shenmen . CM . Schuler . GD . 2002PNAS...9916899M. free .
• Tomsig JL, Snyder SL, Creutz CE . Identification of targets for calcium signaling through the copine family of proteins. Characterization of a coiled-coil copine-binding motif . J. Biol. Chem. . 278 . 12 . 10048–54 . 2003 . 12522145 . 10.1074/jbc.M212632200 . free .
• Huang DT . A unique E1–E2 interaction required for optimal conjugation of the ubiquitin-like protein NEDD8 . Nat. Struct. Mol. Biol. . 11 . 10 . 927–35 . 2004 . 15361859 . 10.1038/nsmb826 . 2862556 . vanc. Miller DW . Mathew R . 3 . Cassell . Robert . Holton . James M . Roussel . Martine F . Schulman . Brenda A .
• Gerhard DS . The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC) . Genome Res. . 14 . 10B . 2121–7 . 2004 . 15489334 . 10.1101/gr.2596504 . 528928 . vanc. Wagner L . Feingold EA . 3 . Shenmen . CM . Grouse . LH . Schuler . G . Klein . SL . Old . S . Rasooly . R .
• Huang DT . Structural basis for recruitment of Ubc12 by an E2 binding domain in NEDD8's E1 . Mol. Cell . 17 . 3 . 341–50 . 2005 . 15694336 . 10.1016/j.molcel.2004.12.020 . vanc. Paydar A . Zhuang M . 3 . Waddell . M.Brett . Holton . James M. . Schulman . Brenda A. . free.
• Li T . A general approach for investigating enzymatic pathways and substrates for ubiquitin-like modifiers . Arch. Biochem. Biophys. . 453 . 1 . 70–4 . 2006 . 16620772 . 10.1016/j.abb.2006.03.002 . vanc. Santockyte R . Shen RF . 3 . Tekle . E . Wang . G . Yang . D . Chock . P .
• Ewing RM . Large-scale mapping of human protein–protein interactions by mass spectrometry . Mol. Syst. Biol. . 3 . 1. 89 . 2007 . 17353931 . 10.1038/msb4100134 . 1847948 . vanc. Chu P . Elisma F . 3 . Li . Hongyan . Taylor . Paul . Climie . Shane . McBroom-Cerajewski . Linda . Robinson . Mark D . O'Connor . Liam .

Notes and References

1. Osaka F, Kawasaki H, Aida N, Saeki M, Chiba T, Kawashima S, Tanaka K, Kato S . A new NEDD8-ligating system for cullin-4A . Genes Dev . 12 . 15 . 2263–8 . Aug 1998 . 9694792 . 317039 . 10.1101/gad.12.15.2263 .
2. Web site: Entrez Gene: UBE2M ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast).
3. Gong L, Yeh ET . Apr 1999 . Identification of the activating and conjugating enzymes of the NEDD8 conjugation pathway . J. Biol. Chem. . 274 . 17 . 12036–42 . UNITED STATES. 0021-9258. 10207026 . 10.1074/jbc.274.17.12036 . free .
4. Ewing RM, Chu P, Elisma F, Li H, Taylor P, Climie S, McBroom-Cerajewski L, Robinson MD, O'Connor L, Li M, Taylor R, Dharsee M, Ho Y, Heilbut A, Moore L, Zhang S, Ornatsky O, Bukhman YV, Ethier M, Sheng Y, Vasilescu J, Abu-Farha M, Lambert JP, Duewel HS, Stewart II, Kuehl B, Hogue K, Colwill K, Gladwish K, Muskat B, Kinach R, Adams SL, Moran MF, Morin GB, Topaloglou T, Figeys D . 2007. Large-scale mapping of human protein–protein interactions by mass spectrometry . Mol. Syst. Biol. . 3 . 1. 89 . England. 17353931 . 10.1038/msb4100134 . 1847948 .
5. Huang DT, Paydar A, Zhuang M, Waddell MB, Holton JM, Schulman BA . Structural basis for recruitment of Ubc12 by an E2 binding domain in NEDD8's E1 . Mol. Cell . 17 . 3 . 341–50 . February 2005 . 15694336 . 10.1016/j.molcel.2004.12.020 . free .