UBE2E1 explained

Ubiquitin-conjugating enzyme E2 E1 is a protein that in humans is encoded by the UBE2E1 gene.^{[1] [2]}

Function

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Interactions

UBE2E1 has been shown to interact with Ataxin 1^[3] and NEDD4.^{[4] [5]}

Further reading

• Pickart CM . Mechanisms underlying ubiquitination . Annu. Rev. Biochem. . 70 . 503–33 . 2001 . 11395416 . 10.1146/annurev.biochem.70.1.503 .
• Chen P, Johnson P, Sommer T, Jentsch S, Hochstrasser M . Multiple ubiquitin-conjugating enzymes participate in the in vivo degradation of the yeast MAT alpha 2 repressor . Cell . 74 . 2 . 357–69 . 1993 . 8393731 . 10.1016/0092-8674(93)90426-Q . 205020910 .
• Anan T, Nagata Y, Koga H, Honda Y, Yabuki N, Miyamoto C, Kuwano A, Matsuda I, Endo F, Saya H, Nakao M . Human ubiquitin-protein ligase Nedd4: expression, subcellular localization and selective interaction with ubiquitin-conjugating enzymes . Genes Cells . 3 . 11 . 751–63 . 1998 . 9990509 . 10.1046/j.1365-2443.1998.00227.x . 1653536 .
• Nyman TA, Matikainen S, Sareneva T, Julkunen I, Kalkkinen N . Proteome analysis reveals ubiquitin-conjugating enzymes to be a new family of interferon-alpha-regulated genes . Eur. J. Biochem. . 267 . 13 . 4011–9 . 2000 . 10866800 . 10.1046/j.1432-1327.2000.01433.x . free .
• Lenk U, Sommer T . Ubiquitin-mediated proteolysis of a short-lived regulatory protein depends on its cellular localization . J. Biol. Chem. . 275 . 50 . 39403–10 . 2000 . 10991948 . 10.1074/jbc.M006949200 . free.
• Pringa E, Martinez-Noel G, Muller U, Harbers K . Interaction of the ring finger-related U-box motif of a nuclear dot protein with ubiquitin-conjugating enzymes . J. Biol. Chem. . 276 . 22 . 19617–23 . 2001 . 11274149 . 10.1074/jbc.M100192200 . free .
• Ito K, Adachi S, Iwakami R, Yasuda H, Muto Y, Seki N, Okano Y . N-Terminally extended human ubiquitin-conjugating enzymes (E2s) mediate the ubiquitination of RING-finger proteins, ARA54 and RNF8 . Eur. J. Biochem. . 268 . 9 . 2725–32 . 2001 . 11322894 . 10.1046/j.1432-1327.2001.02169.x . free .
• Lehner B, Semple JI, Brown SE, Counsell D, Campbell RD, Sanderson CM . Analysis of a high-throughput yeast two-hybrid system and its use to predict the function of intracellular proteins encoded within the human MHC class III region . Genomics . 83 . 1 . 153–67 . 2004 . 14667819 . 10.1016/S0888-7543(03)00235-0 .
• Takeuchi T, Iwahara S, Saeki Y, Sasajima H, Yokosawa H . Link between the ubiquitin conjugation system and the ISG15 conjugation system: ISG15 conjugation to the UbcH6 ubiquitin E2 enzyme . J. Biochem. . 138 . 6 . 711–9 . 2005 . 16428300 . 10.1093/jb/mvi172 .

External links

• PDBe-KB provides an overview of all the structure information available in the PDB for Human Ubiquitin-conjugating enzyme E2 E1 (UBE2E1)

Notes and References

1. Nuber U, Schwarz S, Kaiser P, Schneider R, Scheffner M . Cloning of human ubiquitin-conjugating enzymes UbcH6 and UbcH7 (E2-F1) and characterization of their interaction with E6-AP and RSP5 . J. Biol. Chem. . 271 . 5 . 2795–800 . Mar 1996 . 8576257 . 10.1074/jbc.271.5.2795 . free.
2. Web site: Entrez Gene: UBE2E1 ubiquitin-conjugating enzyme E2E 1 (UBC4/5 homolog, yeast).
3. Hong S, Lee S, Cho SG, Kang S . UbcH6 interacts with and ubiquitinates the SCA1 gene product ataxin-1 . Biochem. Biophys. Res. Commun. . 371 . 2 . 256–60 . Jun 2008 . 18439907 . 10.1016/j.bbrc.2008.04.066 .
4. Anan T, Nagata Y, Koga H, Honda Y, Yabuki N, Miyamoto C, Kuwano A, Matsuda I, Endo F, Saya H, Nakao M . Human ubiquitin-protein ligase Nedd4: expression, subcellular localization and selective interaction with ubiquitin-conjugating enzymes . Genes Cells . 3 . 11 . 751–63 . Nov 1998 . 9990509 . 10.1046/j.1365-2443.1998.00227.x . 1653536 .
5. Malakhova OA, Zhang DE . ISG15 inhibits Nedd4 ubiquitin E3 activity and enhances the innate antiviral response . J. Biol. Chem. . 283 . 14 . 8783–7 . Apr 2008 . 18287095 . 2276364 . 10.1074/jbc.C800030200 . free .