| Tsukubusuma Shrine | |
| Map Type: | Japan |
| Coordinates: | 35.4207°N 136.1442°W |
| Religious Affiliation: | Shinto |
Tsukubusuma Shrine (都久夫須麻神社, Tsukubusuma Jinja) is a Shinto shrine on Chikubu Island in Shiga Prefecture, Japan. Its is a National Treasure of Japan. The shrine's main festival is held annually on June 15. It is also called Chikubushima Shrine (竹生島神社, Chikubushima Jinja).