Trichoderma asperellum Samuels, Lieckf & Nirenberg [1] is a species of fungus in the family Hypocreaceae.[2] It can be distinguished from T. viride by molecular and phenotypic characteristics. The most important molecular characteristics are divergent ITS-1 and 28S sequences and RFLP's of the endochitinase gene. Main phenotypic characters are conidial ornamentation and arrangement and branching of the conidiophores.
This species has been used commercially and experimentally as a biopesticide for plant disease control: some commercial isolates were previously placed in T. harzianum.