TNIK explained

TRAF2 and NCK-interacting protein kinase is an enzyme that in humans is encoded by the TNIK gene.^{[1] [2] [3]}

Function

Germinal center kinases (GCKs), such as TNIK, are characterized by an N-terminal kinase domain and a C-terminal GCK domain that serves a regulatory function.^[3]

Interactions

TNIK has been shown to interact with KIAA0090,^[4] although the significance is unclear. TNIK has been shown to phosphorylate Gelsolin, a protein involved in F-actin depolymerisation thus inducing cytoskeletal changes.

TNIK plays an important role in pulmonary fibrosis. Inhibitors of TNIK are used in the treatment of pulmonary fibrosis. ^[5]

Further reading

• Nakajima D, Okazaki N, Yamakawa H, etal . Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones. . DNA Res. . 9 . 3 . 99–106 . 2003 . 12168954 . 10.1093/dnares/9.3.99 . free .
• Yonekura H, Migita H, Sakurai S, etal . Antisense display--a method for functional gene screening: evaluation in a cell-free system and isolation of angiogenesis-related genes. . Nucleic Acids Res. . 27 . 13 . 2591–600 . 1999 . 10373574 . 10.1093/nar/27.13.2591 . 148466 .
• Strausberg RL, Feingold EA, Grouse LH, etal . Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. . Proc. Natl. Acad. Sci. U.S.A. . 99 . 26 . 16899–903 . 2003 . 12477932 . 10.1073/pnas.242603899 . 139241 . 2002PNAS...9916899M . free .
• Brill LM, Salomon AR, Ficarro SB, etal . Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity chromatography and tandem mass spectrometry. . Anal. Chem. . 76 . 10 . 2763–72 . 2004 . 15144186 . 10.1021/ac035352d .
• Taira K, Umikawa M, Takei K, etal . The Traf2- and Nck-interacting kinase as a putative effector of Rap2 to regulate actin cytoskeleton. . J. Biol. Chem. . 279 . 47 . 49488–96 . 2005 . 15342639 . 10.1074/jbc.M406370200 . free .
• Gerhard DS, Wagner L, Feingold EA, etal . The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). . Genome Res. . 14 . 10B . 2121–7 . 2004 . 15489334 . 10.1101/gr.2596504 . 528928 .
• Wissing J, Jänsch L, Nimtz M, etal . Proteomics analysis of protein kinases by target class-selective prefractionation and tandem mass spectrometry. . Mol. Cell. Proteomics . 6 . 3 . 537–47 . 2007 . 17192257 . 10.1074/mcp.T600062-MCP200 . free . 10033/19756 . free .

Notes and References

1. Nagase T, Ishikawa K, Miyajima N, Tanaka A, Kotani H, Nomura N, Ohara O . Prediction of the coding sequences of unidentified human genes. IX. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro . DNA Res . 5 . 1 . 31–9 . Aug 1998 . 9628581 . 10.1093/dnares/5.1.31 . free .
2. Fu CA, Shen M, Huang BC, Lasaga J, Payan DG, Luo Y . TNIK, a novel member of the germinal center kinase family that activates the c-Jun N-terminal kinase pathway and regulates the cytoskeleton . J Biol Chem . 274 . 43 . 30729–37 . Nov 1999 . 10521462 . 10.1074/jbc.274.43.30729 . free .
3. Web site: Entrez Gene: TNIK TRAF2 and NCK interacting kinase.
4. Prieto C, De Las Rivas J . APID: Agile Protein Interaction DataAnalyzer . Nucleic Acids Res. . 34 . Web Server issue . W298–302 . July 2006 . 16845013 . 1538863 . 10.1093/nar/gkl128 . dead . https://web.archive.org/web/20100409025355/http://bioinfow.dep.usal.es/apid/html/home.htm . 2010-04-09 .
5. Garneau-Tsodikova . Sylvie . Thannickal . Victor J. . 2008 . Protein kinase inhibitors in the treatment of pulmonary fibrosis . Current Medicinal Chemistry . 15 . 25 . 2632–2640 . 10.2174/092986708785908969 . 0929-8673 . 18855683.