TADA2L explained

Transcriptional adapter 2-alpha is a protein that in humans is encoded by the TADA2A gene.^{[1] [2]}

Function

Many DNA-binding transcriptional activator proteins enhance the initiation rate of RNA polymerase II-mediated gene transcription by interacting functionally with the general transcription machinery bound at the basal promoter. Adaptor proteins are usually required for this activation, possibly to acetylate and destabilize nucleosomes, thereby relieving chromatin constraints at the promoter. The protein encoded by this gene is a transcriptional activator adaptor and has been found to be part of the PCAF histone acetylase complex. Two transcript variants encoding different isoforms have been identified for this gene.

Interactions

TADA2L has been shown to interact with GCN5L2,^{[3] [4]} TADA3L^{[5] [6]} and Myc.^[7]

Further reading

• Maruyama K, Sugano S . Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides . Gene . 138 . 1–2 . 171–4 . Jan 1994 . 8125298 . 10.1016/0378-1119(94)90802-8 .
• Wang L, Mizzen C, Ying C, Candau R, Barlev N, Brownell J, Allis CD, Berger SL . Histone acetyltransferase activity is conserved between yeast and human GCN5 and is required for complementation of growth and transcriptional activation . Molecular and Cellular Biology . 17 . 1 . 519–27 . Jan 1997 . 8972232 . 231776 . 10.1128/mcb.17.1.519.
• Carter KC, Wang L, Shell BK, Zamir I, Berger SL, Moore PA . The human transcriptional adaptor genes TADA2L and GCN5L2 colocalize to chromosome 17q12-q21 and display a similar tissue expression pattern . Genomics . 40 . 3 . 497–500 . Mar 1997 . 9073520 . 10.1006/geno.1996.4605 .
• Henriksson A, Almlöf T, Ford J, McEwan IJ, Gustafsson JA, Wright AP . Role of the Ada adaptor complex in gene activation by the glucocorticoid receptor . Molecular and Cellular Biology . 17 . 6 . 3065–73 . Jun 1997 . 9154805 . 232159 . 10.1128/mcb.17.6.3065.
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S . Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library . Gene . 200 . 1–2 . 149–56 . Oct 1997 . 9373149 . 10.1016/S0378-1119(97)00411-3 .
• Ogryzko VV, Kotani T, Zhang X, Schiltz RL, Howard T, Yang XJ, Howard BH, Qin J, Nakatani Y . Histone-like TAFs within the PCAF histone acetylase complex . Cell . 94 . 1 . 35–44 . Jul 1998 . 9674425 . 10.1016/S0092-8674(00)81219-2 . 18942972 . free .
• Vassilev A, Yamauchi J, Kotani T, Prives C, Avantaggiati ML, Qin J, Nakatani Y . The 400 kDa subunit of the PCAF histone acetylase complex belongs to the ATM superfamily . Molecular Cell . 2 . 6 . 869–75 . Dec 1998 . 9885574 . 10.1016/S1097-2765(00)80301-9 . free .
• Wang T, Kobayashi T, Takimoto R, Denes AE, Snyder EL, el-Deiry WS, Brachmann RK . hADA3 is required for p53 activity . The EMBO Journal . 20 . 22 . 6404–13 . Nov 2001 . 11707411 . 125723 . 10.1093/emboj/20.22.6404 .
• Barlev NA, Emelyanov AV, Castagnino P, Zegerman P, Bannister AJ, Sepulveda MA, Robert F, Tora L, Kouzarides T, Birshtein BK, Berger SL . A novel human Ada2 homologue functions with Gcn5 or Brg1 to coactivate transcription . Molecular and Cellular Biology . 23 . 19 . 6944–57 . Oct 2003 . 12972612 . 193946 . 10.1128/MCB.23.19.6944-6957.2003 .
• Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, Berriz GF, Gibbons FD, Dreze M, Ayivi-Guedehoussou N, Klitgord N, Simon C, Boxem M, Milstein S, Rosenberg J, Goldberg DS, Zhang LV, Wong SL, Franklin G, Li S, Albala JS, Lim J, Fraughton C, Llamosas E, Cevik S, Bex C, Lamesch P, Sikorski RS, Vandenhaute J, Zoghbi HY, Smolyar A, Bosak S, Sequerra R, Doucette-Stamm L, Cusick ME, Hill DE, Roth FP, Vidal M . Towards a proteome-scale map of the human protein-protein interaction network . Nature . 437 . 7062 . 1173–8 . Oct 2005 . 16189514 . 10.1038/nature04209 . 2005Natur.437.1173R . 4427026 .
• Qian C, Zhang Q, Li S, Zeng L, Walsh MJ, Zhou MM . Structure and chromosomal DNA binding of the SWIRM domain . Nature Structural & Molecular Biology . 12 . 12 . 1078–85 . Dec 2005 . 16299514 . 10.1038/nsmb1022 . 23215862 .

Notes and References

1. Candau R, Moore PA, Wang L, Barlev N, Ying CY, Rosen CA, Berger SL . Identification of human proteins functionally conserved with the yeast putative adaptors ADA2 and GCN5 . Molecular and Cellular Biology . 16 . 2 . 593–602 . Feb 1996 . 8552087 . 231038 . 10.1128/mcb.16.2.593.
2. Web site: Entrez Gene: TADA2L transcriptional adaptor 2 (ADA2 homolog, yeast)-like.
3. Barlev NA, Poltoratsky V, Owen-Hughes T, Ying C, Liu L, Workman JL, Berger SL . Repression of GCN5 histone acetyltransferase activity via bromodomain-mediated binding and phosphorylation by the Ku-DNA-dependent protein kinase complex . Molecular and Cellular Biology . 18 . 3 . 1349–58 . Mar 1998 . 9488450 . 108848 . 10.1128/mcb.18.3.1349.
4. Wang L, Mizzen C, Ying C, Candau R, Barlev N, Brownell J, Allis CD, Berger SL . Histone acetyltransferase activity is conserved between yeast and human GCN5 and is required for complementation of growth and transcriptional activation . Molecular and Cellular Biology . 17 . 1 . 519–27 . Jan 1997 . 8972232 . 231776 . 10.1128/mcb.17.1.519.
5. Huda Zoghbi . Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, Berriz GF, Gibbons FD, Dreze M, Ayivi-Guedehoussou N, Klitgord N, Simon C, Boxem M, Milstein S, Rosenberg J, Goldberg DS, Zhang LV, Wong SL, Franklin G, Li S, Albala JS, Lim J, Fraughton C, Llamosas E, Cevik S, Bex C, Lamesch P, Sikorski RS, Vandenhaute J, Zoghbi HY, Smolyar A, Bosak S, Sequerra R, Doucette-Stamm L, Cusick ME, Hill DE, Roth FP, Vidal M . Towards a proteome-scale map of the human protein-protein interaction network . Nature . 437 . 7062 . 1173–8 . Oct 2005 . 16189514 . 10.1038/nature04209 . 2005Natur.437.1173R . 4427026 .
6. Wang T, Kobayashi T, Takimoto R, Denes AE, Snyder EL, el-Deiry WS, Brachmann RK . hADA3 is required for p53 activity . The EMBO Journal . 20 . 22 . 6404–13 . Nov 2001 . 11707411 . 125723 . 10.1093/emboj/20.22.6404 .
7. Liu X, Tesfai J, Evrard YA, Dent SY, Martinez E . c-Myc transformation domain recruits the human STAGA complex and requires TRRAP and GCN5 acetylase activity for transcription activation . The Journal of Biological Chemistry . 278 . 22 . 20405–12 . May 2003 . 12660246 . 4031917 . 10.1074/jbc.M211795200 . free .