This is a list of Spring 1981 PGA Tour Qualifying School graduates.
| Player | Notes | ||
|---|---|---|---|
| 1 | Billy Glisson | ||
| T2 | Charles Krenkel | ||
 Gavan Levenson | 1 European Tour win | ||
| T4 | Denis Watson | ||
| Clarence Rose | Winner of 1979 North Carolina Amateur | ||
| Tom Woodward | |||
| 7 | Barry Harwell | ||
| T8 | Charlie Gibson | ||
| Jeff Thomsen | |||
| Skeeter Heath | |||
| T11 | Tom Chain | ||
| Payne Stewart | Winner of 2 Asia Golf Circuit events | ||
| Bob Proben | |||
| Terry Anton | |||
| Tateo Ozaki | |||
| Bill Loeffler | |||
| T17 | Mark Calcavecchia | ||
| Donald Reese | |||
| Rick Borg | |||
| Jim Bertoncino | |||
| Tom Inskeep | |||
| Larry Rinker | Winner of 1978 Southeastern Conference Championship | ||
| T23 | Gene George | ||
| Jeff Sanders | |||
| David Sann |