SART3 explained

Squamous cell carcinoma antigen recognized by T-cells 3 is a protein that in humans is encoded by the SART3 gene.^{[1] [2] [3]}

The protein encoded by this gene is an RNA-binding nuclear protein that is a tumor-rejection antigen. This antigen possesses tumor epitopes capable of inducing HLA-A24-restricted and tumor-specific cytotoxic T lymphocytes in cancer patients and may be useful for specific immunotherapy. This gene product is found to be an important cellular factor for HIV-1 gene expression and viral replication. It also associates transiently with U6 and U4/U6 snRNPs during the recycling phase of the spliceosome cycle. This encoded protein is thought to be involved in the regulation of mRNA splicing.^[3]

Interactions

SART3 has been shown to interact with RNPS1^[4] and Androgen receptor.^[5]

Further reading

• Maruyama K, Sugano S . Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides . Gene . 138 . 1–2 . 171–4 . January 1994 . 8125298 . 10.1016/0378-1119(94)90802-8 .
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S . Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library . Gene . 200 . 1–2 . 149–56 . October 1997 . 9373149 . 10.1016/S0378-1119(97)00411-3 .
• Yang D, Nakao M, Shichijo S, Sasatomi T, Takasu H, Matsumoto H, Mori K, Hayashi A, Yamana H, Shirouzu K, Itoh K . Identification of a gene coding for a protein possessing shared tumor epitopes capable of inducing HLA-A24-restricted cytotoxic T lymphocytes in cancer patients . Cancer Research . 59 . 16 . 4056–63 . August 1999 . 10463607 .
• Tanaka S, Tsuda N, Kawano K, Sakamoto M, Nishida T, Hashimoto T, Shichijo S, Kamura T, Itoh K . Expression of tumor-rejection antigens in gynecologic cancers . Japanese Journal of Cancer Research . 91 . 11 . 1177–84 . November 2000 . 11092984 . 5926290 . 10.1111/j.1349-7006.2000.tb00902.x .
• Harada K, Yamada A, Yang D, Itoh K, Shichijo S . Binding of a SART3 tumor-rejection antigen to a pre-mRNA splicing factor RNPS1: a possible regulation of splicing by a complex formation . International Journal of Cancer . 93 . 5 . 623–8 . September 2001 . 11477570 . 10.1002/ijc.1391 . 24724555 . free .
• Sasatomi T, Suefuji Y, Matsunaga K, Yamana H, Miyagi Y, Araki Y, Ogata Y, Itoh K, Shirouzu K . Expression of tumor rejection antigens in colorectal carcinomas . Cancer . 94 . 6 . 1636–41 . March 2002 . 11920522 . 10.1002/cncr.10421 . 33045822 . free .
• Liu Y, Li J, Kim BO, Pace BS, He JJ . HIV-1 Tat protein-mediated transactivation of the HIV-1 long terminal repeat promoter is potentiated by a novel nuclear Tat-interacting protein of 110 kDa, Tip110 . The Journal of Biological Chemistry . 277 . 26 . 23854–63 . June 2002 . 11959860 . 10.1074/jbc.M200773200 . free .
• Bell M, Schreiner S, Damianov A, Reddy R, Bindereif A . p110, a novel human U6 snRNP protein and U4/U6 snRNP recycling factor . The EMBO Journal . 21 . 11 . 2724–35 . June 2002 . 12032085 . 126028 . 10.1093/emboj/21.11.2724 .
• Nottrott S, Urlaub H, Lührmann R . Hierarchical, clustered protein interactions with U4/U6 snRNA: a biochemical role for U4/U6 proteins . The EMBO Journal . 21 . 20 . 5527–38 . October 2002 . 12374753 . 129076 . 10.1093/emboj/cdf544 .
• Nakayama M, Kikuno R, Ohara O . Protein-protein interactions between large proteins: two-hybrid screening using a functionally classified library composed of long cDNAs . Genome Research . 12 . 11 . 1773–84 . November 2002 . 12421765 . 187542 . 10.1101/gr.406902 .
• Stanĕk D, Rader SD, Klingauf M, Neugebauer KM . Targeting of U4/U6 small nuclear RNP assembly factor SART3/p110 to Cajal bodies . The Journal of Cell Biology . 160 . 4 . 505–16 . February 2003 . 12578909 . 2173746 . 10.1083/jcb.200210087 .
• Damianov A, Schreiner S, Bindereif A . Recycling of the U12-type spliceosome requires p110, a component of the U6atac snRNP . Molecular and Cellular Biology . 24 . 4 . 1700–8 . February 2004 . 14749385 . 344176 . 10.1128/MCB.24.4.1700-1708.2004 .
• Liu Y, Kim BO, Kao C, Jung C, Dalton JT, He JJ . Tip110, the human immunodeficiency virus type 1 (HIV-1) Tat-interacting protein of 110 kDa as a negative regulator of androgen receptor (AR) transcriptional activation . The Journal of Biological Chemistry . 279 . 21 . 21766–73 . May 2004 . 15031286 . 10.1074/jbc.M314321200 . free .
• Brandenberger R, Wei H, Zhang S, Lei S, Murage J, Fisk GJ, Li Y, Xu C, Fang R, Guegler K, Rao MS, Mandalam R, Lebkowski J, Stanton LW . Transcriptome characterization elucidates signaling networks that control human ES cell growth and differentiation . Nature Biotechnology . 22 . 6 . 707–16 . June 2004 . 15146197 . 10.1038/nbt971 . 27764390 .
• Medenbach J, Schreiner S, Liu S, Lührmann R, Bindereif A . Human U4/U6 snRNP recycling factor p110: mutational analysis reveals the function of the tetratricopeptide repeat domain in recycling . Molecular and Cellular Biology . 24 . 17 . 7392–401 . September 2004 . 15314151 . 506986 . 10.1128/MCB.24.17.7392-7401.2004 .
• Stanĕk D, Neugebauer KM . Detection of snRNP assembly intermediates in Cajal bodies by fluorescence resonance energy transfer . The Journal of Cell Biology . 166 . 7 . 1015–25 . September 2004 . 15452143 . 2172029 . 10.1083/jcb.200405160 .

Notes and References

1. Nagase T, Seki N, Tanaka A, Ishikawa K, Nomura N . Prediction of the coding sequences of unidentified human genes. IV. The coding sequences of 40 new genes (KIAA0121-KIAA0160) deduced by analysis of cDNA clones from human cell line KG-1 . DNA Research . 2 . 4 . 167–74, 199–210 . August 1995 . 8590280 . 10.1093/dnares/2.4.167 . free .
2. Harada K, Yamada A, Mine T, Kawagoe N, Takasu H, Itoh K . Mouse homologue of the human SART3 gene encoding tumor-rejection antigen . Japanese Journal of Cancer Research . 91 . 2 . 239–47 . February 2000 . 10761712 . 5926322. 10.1111/j.1349-7006.2000.tb00937.x .
3. Web site: Entrez Gene: SART3 squamous cell carcinoma antigen recognized by T cells 3.
4. Harada K, Yamada A, Yang D, Itoh K, Shichijo S . Binding of a SART3 tumor-rejection antigen to a pre-mRNA splicing factor RNPS1: a possible regulation of splicing by a complex formation . International Journal of Cancer . 93 . 5 . 623–8 . September 2001 . 11477570 . 10.1002/ijc.1391 . 24724555 . free .
5. Liu Y, Kim BO, Kao C, Jung C, Dalton JT, He JJ . Tip110, the human immunodeficiency virus type 1 (HIV-1) Tat-interacting protein of 110 kDa as a negative regulator of androgen receptor (AR) transcriptional activation . The Journal of Biological Chemistry . 279 . 21 . 21766–73 . May 2004 . 15031286 . 10.1074/jbc.M314321200 . free .