SAP18 explained

Histone deacetylase complex subunit SAP18 is an enzyme that in humans is encoded by the SAP18 gene.^{[1] [2]}

Function

Histone acetylation plays a key role in the regulation of eukaryotic gene expression. Multisubunit complexes catalyze histone acetylation and deacetylation. The protein encoded by this gene is a histone deacetylase complex component, including SIN3, SAP30, HDAC1, HDAC2, RbAp46, RbAp48, and other polypeptides. This protein directly interacts with SIN3 and enhances SIN3-mediated transcriptional repression when tethered to the promoter. Additionally, SAP18s splice variants are implicated in apoptotic cycles.^[3]

Interactions

SAP18 has been shown to interact with

• POLE2,^{[4] [5]} and
• RGS10.^[6]

Further reading

• Koipally J, Renold A, Kim J, Georgopoulos K . Repression by Ikaros and Aiolos is mediated through histone deacetylase complexes . The EMBO Journal . 18 . 11 . 3090–100 . June 1999 . 10357820 . 1171390 . 10.1093/emboj/18.11.3090 .
• Wada M, Miyazawa H, Wang RS, Mizuno T, Sato A, Asashima M, Hanaoka F . The second largest subunit of mouse DNA polymerase epsilon, DPE2, interacts with SAP18 and recruits the Sin3 co-repressor protein to DNA . Journal of Biochemistry . 131 . 3 . 307–11 . March 2002 . 11872158 . 10.1093/oxfordjournals.jbchem.a003104 .
• Cheng SY, Bishop JM . Suppressor of Fused represses Gli-mediated transcription by recruiting the SAP18-mSin3 corepressor complex . Proceedings of the National Academy of Sciences of the United States of America . 99 . 8 . 5442–7 . April 2002 . 11960000 . 122788 . 10.1073/pnas.082096999 . 2002PNAS...99.5442C . free .
• Paces-Fessy M, Boucher D, Petit E, Paute-Briand S, Blanchet-Tournier MF . The negative regulator of Gli, Suppressor of fused (Sufu), interacts with SAP18, Galectin3 and other nuclear proteins . The Biochemical Journal . 378 . Pt 2 . 353–62 . March 2004 . 14611647 . 1223961 . 10.1042/BJ20030786 .
• Andersen JS, Lam YW, Leung AK, Ong SE, Lyon CE, Lamond AI, Mann M . Nucleolar proteome dynamics . Nature . 433 . 7021 . 77–83 . January 2005 . 15635413 . 10.1038/nature03207 . 2005Natur.433...77A . 4344740 .

Notes and References

1. Zhang Y, Iratni R, Erdjument-Bromage H, Tempst P, Reinberg D . Histone deacetylases and SAP18, a novel polypeptide, are components of a human Sin3 complex . Cell . 89 . 3 . 357–64 . May 1997 . 9150135 . 10.1016/S0092-8674(00)80216-0 . free .
2. Web site: Entrez Gene: SAP18 Sin3A-associated protein, 18kDa.
3. Michelle L, Cloutier A, Toutant J, Shkreta L, Thibault P, Durand M, Garneau D, Gendron D, Lapointe E, Couture S, Le Hir H, Klinck R, Elela SA, Prinos P, Chabot B . Proteins associated with the exon junction complex also control the alternative splicing of apoptotic regulators . Molecular and Cellular Biology . 32 . 5 . 954–67 . March 2012 . 22203037 . 3295189 . 10.1128/MCB.06130-11 .
4. Wada M, Miyazawa H, Wang RS, Mizuno T, Sato A, Asashima M, Hanaoka F . The second largest subunit of mouse DNA polymerase epsilon, DPE2, interacts with SAP18 and recruits the Sin3 co-repressor protein to DNA . Journal of Biochemistry . 131 . 3 . 307–11 . March 2002 . 11872158 . 10.1093/oxfordjournals.jbchem.a003104 .
5. Cheng SY, Bishop JM . Suppressor of Fused represses Gli-mediated transcription by recruiting the SAP18-mSin3 corepressor complex . Proceedings of the National Academy of Sciences of the United States of America . 99 . 8 . 5442–7 . April 2002 . 11960000 . 122788 . 10.1073/pnas.082096999 . 2002PNAS...99.5442C . free .
6. Ewing RM, Chu P, Elisma F, Li H, Taylor P, Climie S, McBroom-Cerajewski L, Robinson MD, O'Connor L, Li M, Taylor R, Dharsee M, Ho Y, Heilbut A, Moore L, Zhang S, Ornatsky O, Bukhman YV, Ethier M, Sheng Y, Vasilescu J, Abu-Farha M, Lambert JP, Duewel HS, Stewart II, Kuehl B, Hogue K, Colwill K, Gladwish K, Muskat B, Kinach R, Adams SL, Moran MF, Morin GB, Topaloglou T, Figeys D . Large-scale mapping of human protein-protein interactions by mass spectrometry . Molecular Systems Biology . 3 . 1 . 89 . 2007 . 17353931 . 1847948 . 10.1038/msb4100134 .