S-specific spore photoproduct lyase explained

S-specific spore photoproduct lyase (SAM, SP lyase, SPL, SplB, SplG) is an enzyme with systematic name S-specific spore photoproduct pyrimidine-lyase.^{[1] [2] [3] [4]} This enzyme catalyses the following chemical reaction

(5S)-5,6-dihydro-5-(thymidin-7-yl)thymidine (in DNA) + S-adenosyl-L-methionine

thymidylyl-(3'->5')-thymidylate (in DNA) + 5'-deoxyadenosine + L-methionine

This enzyme is an iron-sulfur protein.

Notes and References

1. Chandor A, Berteau O, Douki T, Gasparutto D, Sanakis Y, Ollagnier-de-Choudens S, Atta M, Fontecave M . Dinucleotide spore photoproduct, a minimal substrate of the DNA repair spore photoproduct lyase enzyme from Bacillus subtilis . The Journal of Biological Chemistry . 281 . 37 . 26922–31 . September 2006 . 16829676 . 10.1074/jbc.m602297200 . free .
2. Pieck JC, Hennecke U, Pierik AJ, Friedel MG, Carell T . Characterization of a new thermophilic spore photoproduct lyase from Geobacillus stearothermophilus (SplG) with defined lesion containing DNA substrates . The Journal of Biological Chemistry . 281 . 47 . 36317–26 . November 2006 . 16968710 . 10.1074/jbc.m607053200 . free .
3. Mehl RA, Begley TP . Mechanistic studies on the repair of a novel DNA photolesion: the spore photoproduct . Organic Letters . 1 . 7 . 1065–6 . October 1999 . 10825958 . 10.1021/ol9908676 .
4. Friedel MG, Berteau O, Pieck JC, Atta M, Ollagnier-de-Choudens S, Fontecave M, Carell T . The spore photoproduct lyase repairs the 5S- and not the 5R-configured spore photoproduct DNA lesion . Chemical Communications . 4 . 445–7 . January 2006 . 16493831 . 10.1039/b514103f .