RCHY1 explained

RING finger and CHY zinc finger domain-containing protein 1 is a protein that in humans is encoded by the RCHY1 gene.^[1]

Function

The protein encoded by this gene has ubiquitin-protein ligase activity. This protein binds with p53 and promotes the ubiquitin-mediated proteosomal degradation of p53. This gene is oncogenic because loss of p53 function contributes directly to malignant tumor development. Transcription of this gene is regulated by p53. Alternative splicing results in multiple transcript variants encoding different isoforms.

Interactions

RCHY1 has been shown to interact with P53^{[2] [3]} and Androgen receptor.^[4]

Further reading

• Bonaldo MF, Lennon G, Soares MB . Normalization and subtraction: two approaches to facilitate gene discovery . Genome Research . 6 . 9 . 791–806 . Sep 1996 . 8889548 . 10.1101/gr.6.9.791 . free .
• Beitel LK, Elhaji YA, Lumbroso R, Wing SS, Panet-Raymond V, Gottlieb B, Pinsky L, Trifiro MA . Cloning and characterization of an androgen receptor N-terminal-interacting protein with ubiquitin-protein ligase activity . Journal of Molecular Endocrinology . 29 . 1 . 41–60 . Aug 2002 . 12200228 . 10.1677/jme.0.0290041 . free .
• Leng RP, Lin Y, Ma W, Wu H, Lemmers B, Chung S, Parant JM, Lozano G, Hakem R, Benchimol S . Pirh2, a p53-induced ubiquitin-protein ligase, promotes p53 degradation . Cell . 112 . 6 . 779–91 . Mar 2003 . 12654245 . 10.1016/S0092-8674(03)00193-4 . 9316769 . free .
• Logan IR, Sapountzi V, Gaughan L, Neal DE, Robson CN . Control of human PIRH2 protein stability: involvement of TIP60 and the proteosome . The Journal of Biological Chemistry . 279 . 12 . 11696–704 . Mar 2004 . 14701804 . 10.1074/jbc.M312712200 . free .
• Corcoran CA, Huang Y, Sheikh MS . The p53 paddy wagon: COP1, Pirh2 and MDM2 are found resisting apoptosis and growth arrest . Cancer Biology & Therapy . 3 . 8 . 721–5 . Aug 2004 . 15280670 . 10.4161/cbt.3.8.1068 . free .
• Duan W, Gao L, Druhan LJ, Zhu WG, Morrison C, Otterson GA, Villalona-Calero MA . Expression of Pirh2, a newly identified ubiquitin protein ligase, in lung cancer . Journal of the National Cancer Institute . 96 . 22 . 1718–21 . Nov 2004 . 15547185 . 10.1093/jnci/djh292 . free .
• Zhang L, Li J, Wang C, Ma Y, Huo K . A new human gene hNTKL-BP1 interacts with hPirh2 . Biochemical and Biophysical Research Communications . 330 . 1 . 293–7 . Apr 2005 . 15781263 . 10.1016/j.bbrc.2005.02.156 .
• Logan IR, Gaughan L, McCracken SR, Sapountzi V, Leung HY, Robson CN . Human PIRH2 enhances androgen receptor signaling through inhibition of histone deacetylase 1 and is overexpressed in prostate cancer . Molecular and Cellular Biology . 26 . 17 . 6502–10 . Sep 2006 . 16914734 . 1592843 . 10.1128/MCB.00147-06 .
• Duan W, Gao L, Wu X, Zhang Y, Otterson GA, Villalona-Calero MA . Differential response between the p53 ubiquitin-protein ligases Pirh2 and MdM2 following DNA damage in human cancer cells . Experimental Cell Research . 312 . 17 . 3370–8 . Oct 2006 . 16934800 . 10.1016/j.yexcr.2006.07.005 .
• Duan S, Yao Z, Hou D, Wu Z, Zhu WG, Wu M . Phosphorylation of Pirh2 by calmodulin-dependent kinase II impairs its ability to ubiquitinate p53 . The EMBO Journal . 26 . 13 . 3062–74 . Jul 2007 . 17568776 . 1914097 . 10.1038/sj.emboj.7601749 .
• Hattori T, Isobe T, Abe K, Kikuchi H, Kitagawa K, Oda T, Uchida C, Kitagawa M . Pirh2 promotes ubiquitin-dependent degradation of the cyclin-dependent kinase inhibitor p27Kip1 . Cancer Research . 67 . 22 . 10789–95 . Nov 2007 . 18006823 . 10.1158/0008-5472.CAN-07-2033 . free .

Notes and References

1. Web site: Entrez Gene: RCHY1 ring finger and CHY zinc finger domain containing 1.
2. Leng RP, Lin Y, Ma W, Wu H, Lemmers B, Chung S, Parant JM, Lozano G, Hakem R, Benchimol S . Pirh2, a p53-induced ubiquitin-protein ligase, promotes p53 degradation . Cell . 112 . 6 . 779–91 . Mar 2003 . 12654245 . 10.1016/S0092-8674(03)00193-4 . 9316769 . free .
3. Sheng Y, Laister RC, Lemak A, Wu B, Tai E, Duan S, Lukin J, Sunnerhagen M, Srisailam S, Karra M, Benchimol S, Arrowsmith CH . Molecular basis of Pirh2-mediated p53 ubiquitylation . Nature Structural & Molecular Biology . 15 . 12 . 1334–42 . Dec 2008 . 19043414 . 10.1038/nsmb.1521 . 4075976.
4. Beitel LK, Elhaji YA, Lumbroso R, Wing SS, Panet-Raymond V, Gottlieb B, Pinsky L, Trifiro MA . Cloning and characterization of an androgen receptor N-terminal-interacting protein with ubiquitin-protein ligase activity . Journal of Molecular Endocrinology . 29 . 1 . 41–60 . Aug 2002 . 12200228 . 10.1677/jme.0.0290041 . free .