RCC1 explained

Regulator of chromosome condensation 1, also known as RCC1, Ran guanine nucleotide exchange factor and RanGEF, is the name for a human gene and protein.^[1]

RCC1 also functions as a guanine nucleotide exchange factor for Ran GTPase.

Interactions

RCC1 has been shown to interact with RANBP3^{[2] [3]} and Ran (biology).^{[4] [5] [6] [7]}

Further reading

• Furuno N, Nakagawa K, Eguchi U, Ohtsubo M, Nishimoto T, Soeda E, Ohtubo M . Complete nucleotide sequence of the human RCC1 gene involved in coupling between DNA replication and mitosis . Genomics . 11 . 2 . 459–61 . October 1991 . 1769659 . 10.1016/0888-7543(91)90156-9 .
• Bischoff FR, Ponstingl H . Catalysis of guanine nucleotide exchange on Ran by the mitotic regulator RCC1 . Nature . 354 . 6348 . 80–2 . November 1991 . 1944575 . 10.1038/354080a0 . 1991Natur.354...80B . 4346596 .
• Bischoff FR, Ponstingl H . Mitotic regulator protein RCC1 is complexed with a nuclear ras-related polypeptide . Proceedings of the National Academy of Sciences of the United States of America . 88 . 23 . 10830–4 . December 1991 . 1961752 . 53025 . 10.1073/pnas.88.23.10830 . 1991PNAS...8810830B . free .
• Bischoff FR, Maier G, Tilz G, Ponstingl H . A 47-kDa human nuclear protein recognized by antikinetochore autoimmune sera is homologous with the protein encoded by RCC1, a gene implicated in onset of chromosome condensation . Proceedings of the National Academy of Sciences of the United States of America . 87 . 21 . 8617–21 . November 1990 . 2236072 . 55008 . 10.1073/pnas.87.21.8617 . 1990PNAS...87.8617B . free .
• Ohtsubo M, Okazaki H, Nishimoto T . The RCC1 protein, a regulator for the onset of chromosome condensation locates in the nucleus and binds to DNA . The Journal of Cell Biology . 109 . 4 Pt 1 . 1389–97 . October 1989 . 2677018 . 2115805 . 10.1083/jcb.109.4.1389 .
• Ohtsubo M, Kai R, Furuno N, Sekiguchi T, Sekiguchi M, Hayashida H, Kuma K, Miyata T, Fukushige S, Murotsu T . Isolation and characterization of the active cDNA of the human cell cycle gene (RCC1) involved in the regulation of onset of chromosome condensation . Genes & Development . 1 . 6 . 585–93 . August 1987 . 3678831 . 10.1101/gad.1.6.585 . free .
• Hayashi N, Yokoyama N, Seki T, Azuma Y, Ohba T, Nishimoto T . RanBP1, a Ras-like nuclear G protein binding to Ran/TC4, inhibits RCC1 via Ran/TC4 . Molecular & General Genetics . 247 . 6 . 661–9 . June 1995 . 7616957 . 10.1007/BF00290397 . 24266692 .
• Nishimoto T, Seino H, Seki N, Hori TA . The human CHC1 gene encoding RCC1 (regulator of chromosome condensation) (CHC1) is localized to human chromosome 1p36.1 . Genomics . 23 . 3 . 719–21 . October 1994 . 7851910 . 10.1006/geno.1994.1570 .
• Miyabashira J, Sekiguchi T, Nishimoto T . Mammalian cells have two functional RCC1 proteins produced by alternative splicing . Journal of Cell Science . 107 (Pt 8) . 8 . 2203–8 . August 1994 . 10.1242/jcs.107.8.2203 . 7983178 .
• Noguchi E, Hayashi N, Azuma Y, Seki T, Nakamura M, Nakashima N, Yanagida M, He X, Mueller U, Sazer S, Nishimoto T . Dis3, implicated in mitotic control, binds directly to Ran and enhances the GEF activity of RCC1 . The EMBO Journal . 15 . 20 . 5595–605 . October 1996 . 8896453 . 452304 . 10.1002/j.1460-2075.1996.tb00944.x .
• Renault L, Nassar N, Vetter I, Becker J, Klebe C, Roth M, Wittinghofer A . The 1.7 A crystal structure of the regulator of chromosome condensation (RCC1) reveals a seven-bladed propeller . Nature . 392 . 6671 . 97–101 . March 1998 . 9510255 . 10.1038/32204 . 1998Natur.392...97R . 4344559 .
• Renault L, Nassar N, Wittinghofer A, Roth M, Vetter IR . Crystallization and preliminary X-ray analysis of human RCC1, the regulator of chromosome condensation . Acta Crystallographica. Section D, Biological Crystallography . 55 . Pt 1 . 272–5 . January 1999 . 10089422 . 10.1107/S0907444998007768 . 1999AcCrD..55..272R .
• Azuma Y, Renault L, García-Ranea JA, Valencia A, Nishimoto T, Wittinghofer A . Model of the ran-RCC1 interaction using biochemical and docking experiments . Journal of Molecular Biology . 289 . 4 . 1119–30 . June 1999 . 10369786 . 10.1006/jmbi.1999.2820 .
• Talcott B, Moore MS . The nuclear import of RCC1 requires a specific nuclear localization sequence receptor, karyopherin alpha3/Qip . The Journal of Biological Chemistry . 275 . 14 . 10099–104 . April 2000 . 10744690 . 10.1074/jbc.275.14.10099 . free .
• Nemergut ME, Macara IG . Nuclear import of the ran exchange factor, RCC1, is mediated by at least two distinct mechanisms . The Journal of Cell Biology . 149 . 4 . 835–50 . May 2000 . 10811825 . 2174574 . 10.1083/jcb.149.4.835 .
• Fontoura BM, Blobel G, Yaseen NR . The nucleoporin Nup98 is a site for GDP/GTP exchange on ran and termination of karyopherin beta 2-mediated nuclear import . The Journal of Biological Chemistry . 275 . 40 . 31289–96 . October 2000 . 10875935 . 10.1074/jbc.M004651200 . free .
• Freire J, Covelo G, Sarandeses C, Díaz-Jullien C, Freire M . Identification of nuclear-import and cell-cycle regulatory proteins that bind to prothymosin alpha . Biochemistry and Cell Biology . 79 . 2 . 123–31 . 2001 . 11310559 . 10.1139/bcb-79-2-123 .
• Renault L, Kuhlmann J, Henkel A, Wittinghofer A . Structural basis for guanine nucleotide exchange on Ran by the regulator of chromosome condensation (RCC1) . Cell . 105 . 2 . 245–55 . April 2001 . 11336674 . 10.1016/S0092-8674(01)00315-4 . 12827419 . free .
• Nemergut ME, Mizzen CA, Stukenberg T, Allis CD, Macara IG . Chromatin docking and exchange activity enhancement of RCC1 by histones H2A and H2B . Science . 292 . 5521 . 1540–3 . May 2001 . 11375490 . 10.1126/science.292.5521.1540 . 2001Sci...292.1540N .
• Nemergut ME, Lindsay ME, Brownawell AM, Macara IG . Ran-binding protein 3 links Crm1 to the Ran guanine nucleotide exchange factor . The Journal of Biological Chemistry . 277 . 20 . 17385–8 . May 2002 . 11932251 . 10.1074/jbc.C100620200 . free .

Notes and References

1. Web site: Entrez Gene: RCC1 Regulator of chromosome condensation 1.
2. Nemergut ME, Lindsay ME, Brownawell AM, Macara IG . Ran-binding protein 3 links Crm1 to the Ran guanine nucleotide exchange factor . The Journal of Biological Chemistry . 277 . 20 . 17385–8 . May 2002 . 11932251 . 10.1074/jbc.C100620200 . free .
3. Mueller L, Cordes VC, Bischoff FR, Ponstingl H . Human RanBP3, a group of nuclear RanGTP binding proteins . FEBS Letters . 427 . 3 . 330–6 . May 1998 . 9637251 . 10.1016/S0014-5793(98)00459-1 . free .
4. Steggerda SM, Paschal BM . The mammalian Mog1 protein is a guanine nucleotide release factor for Ran . The Journal of Biological Chemistry . 275 . 30 . 23175–80 . July 2000 . 10811801 . 10.1074/jbc.C000252200 . free .
5. Renault L, Kuhlmann J, Henkel A, Wittinghofer A . Structural basis for guanine nucleotide exchange on Ran by the regulator of chromosome condensation (RCC1) . Cell . 105 . 2 . 245–55 . April 2001 . 11336674 . 10.1016/S0092-8674(01)00315-4 . 12827419 . free .
6. Azuma Y, Renault L, García-Ranea JA, Valencia A, Nishimoto T, Wittinghofer A . Model of the ran-RCC1 interaction using biochemical and docking experiments . Journal of Molecular Biology . 289 . 4 . 1119–30 . June 1999 . 10369786 . 10.1006/jmbi.1999.2820 .
7. Ren M, Villamarin A, Shih A, Coutavas E, Moore MS, LoCurcio M, Clarke V, Oppenheim JD, D'Eustachio P, Rush MG . Separate domains of the Ran GTPase interact with different factors to regulate nuclear protein import and RNA processing . Molecular and Cellular Biology . 15 . 4 . 2117–24 . April 1995 . 7891706 . 230439 . 10.1128/MCB.15.4.2117 .