RAB3A explained

Ras-related protein Rab-3A is a protein that in humans is encoded by the RAB3A gene.^{[1] [2] [3]} It is involved in calcium-triggered exocytosis in neurons.

Interactions

RAB3A has been shown to interact with:

• RIMS1,^[4]
• UNC13A,^[5]
• RPH3A,^{[6] [7] [8]} and
• CHM.^{[9] [10]}

Further reading

• Khosravi-Far R, Lutz RJ, Cox AD, Conroy L, Bourne JR, Sinensky M, Balch WE, Buss JE, Der CJ . Isoprenoid modification of rab proteins terminating in CC or CXC motifs. . Proc. Natl. Acad. Sci. U.S.A. . 88 . 14 . 6264–8 . 1991 . 1648736 . 52063 . 10.1073/pnas.88.14.6264 . 1991PNAS...88.6264K . free .
• Zahraoui A, Touchot N, Chardin P, Tavitian A . The human Rab genes encode a family of GTP-binding proteins related to yeast YPT1 and SEC4 products involved in secretion. . J. Biol. Chem. . 264 . 21 . 12394–401 . 1989 . 10.1016/S0021-9258(18)63872-4 . 2501306 . free .
• Farnsworth CC, Seabra MC, Ericsson LH, Gelb MH, Glomset JA . Rab geranylgeranyl transferase catalyzes the geranylgeranylation of adjacent cysteines in the small GTPases Rab1A, Rab3A, and Rab5A. . Proc. Natl. Acad. Sci. U.S.A. . 91 . 25 . 11963–7 . 1995 . 7991565 . 45356 . 10.1073/pnas.91.25.11963 . 1994PNAS...9111963F . free .
• McKiernan CJ, Brondyk WH, Macara IG . The Rab3A GTPase interacts with multiple factors through the same effector domain. Mutational analysis of cross-linking of Rab3A to a putative target protein. . J. Biol. Chem. . 268 . 32 . 24449–52 . 1993 . 10.1016/S0021-9258(20)80546-8 . 8226995 . free .
• Trask B, Fertitta A, Christensen M, Youngblom J, Bergmann A, Copeland A, de Jong P, Mohrenweiser H, Olsen A, Carrano A . Fluorescence in situ hybridization mapping of human chromosome 19: cytogenetic band location of 540 cosmids and 70 genes or DNA markers. . Genomics . 15 . 1 . 133–45 . 1993 . 8432525 . 10.1006/geno.1993.1021 .
• Johannes L, Perez F, Laran-Chich MP, Henry JP, Darchen F . Characterization of the interaction of the monomeric GTP-binding protein Rab3a with geranylgeranyl transferase II. . Eur. J. Biochem. . 239 . 2 . 362–8 . 1996 . 8706741 . 10.1111/j.1432-1033.1996.0362u.x .
• Burton JL, Slepnev V, De Camilli PV . An evolutionarily conserved domain in a subfamily of Rabs is crucial for the interaction with the guanyl nucleotide exchange factor Mss4. . J. Biol. Chem. . 272 . 6 . 3663–8 . 1997 . 9013620 . 10.1074/jbc.272.6.3663 . free .
• Geppert M, Goda Y, Stevens CF, Südhof TC . The small GTP-binding protein Rab3A regulates a late step in synaptic vesicle fusion. . Nature . 387 . 6635 . 810–4 . 1997 . 9194562 . 10.1038/42954 . 4359038 . free .
• Martincic I, Peralta ME, Ngsee JK . Isolation and characterization of a dual prenylated Rab and VAMP2 receptor. . J. Biol. Chem. . 272 . 43 . 26991–8 . 1997 . 9341137 . 10.1074/jbc.272.43.26991 . free.
• Ostermeier C, Brunger AT . Structural basis of Rab effector specificity: crystal structure of the small G protein Rab3A complexed with the effector domain of rabphilin-3A. . Cell . 96 . 3 . 363–74 . 1999 . 10025402 . 10.1016/S0092-8674(00)80549-8 . 15162326 . free .
• Jung YJ, Lee TH, Lee JY, Kim JH, Park JB . Phosphatidic acid is important to the translocation of Rab3A from the cytosol to phospholipid membranes. . NeuroReport . 10 . 13 . 2859–63 . 1999 . 10511453 . 10.1097/00001756-199909090-00029 .
• Sullivan M, Olsen AS, Houslay MD . Genomic organisation of the human cyclic AMP-specific phosphodiesterase PDE4C gene and its chromosomal localisation to 19p13.1, between RAB3A and JUND. . Cell. Signal. . 11 . 10 . 735–42 . 2000 . 10574328 . 10.1016/S0898-6568(99)00037-6 .
• Clabecq A, Henry JP, Darchen F . Biochemical characterization of Rab3-GTPase-activating protein reveals a mechanism similar to that of Ras-GAP. . J. Biol. Chem. . 275 . 41 . 31786–91 . 2000 . 10859313 . 10.1074/jbc.M003705200 . free .
• Haynes LP, Evans GJ, Morgan A, Burgoyne RD . A direct inhibitory role for the Rab3-specific effector, Noc2, in Ca2+-regulated exocytosis in neuroendocrine cells. . J. Biol. Chem. . 276 . 13 . 9726–32 . 2001 . 11134008 . 10.1074/jbc.M006959200 . free .
• Zhang Y, Luan Z, Liu A, Hu G . The scaffolding protein CASK mediates the interaction between rabphilin3a and beta-neurexins. . FEBS Lett. . 497 . 2–3 . 99–102 . 2001 . 11377421 . 10.1016/S0014-5793(01)02450-4 . 33119468 .
• Luo HR, Saiardi A, Nagata E, Ye K, Yu H, Jung TS, Luo X, Jain S, Sawa A, Snyder SH . GRAB: a physiologic guanine nucleotide exchange factor for Rab3A, which interacts with inositol hexakisphosphate kinase. . Neuron . 31 . 3 . 439–51 . 2001 . 11516400 . 10.1016/S0896-6273(01)00384-1 . 10990373 . free .
• Piiper A, Leser J, Lutz MP, Beil M, Zeuzem S . Subcellular distribution and function of Rab3A-D in pancreatic acinar AR42J cells. . Biochem. Biophys. Res. Commun. . 287 . 3 . 746–51 . 2001 . 11563859 . 10.1006/bbrc.2001.5651 .
• Kuroda TS, Fukuda M, Ariga H, Mikoshiba K . The Slp homology domain of synaptotagmin-like proteins 1-4 and Slac2 functions as a novel Rab27A binding domain. . J. Biol. Chem. . 277 . 11 . 9212–8 . 2002 . 11773082 . 10.1074/jbc.M112414200 . free .

Notes and References

1. Rousseau-Merck MF, Zahraoui A, Bernheim A, Touchot N, Miglierina R, Tavitian A, Berger R . Chromosome mapping of the human ras-related rab3A gene to 19p13.2 . Genomics . 5 . 4 . 694–8 . January 1990 . 2687157 . 10.1016/0888-7543(89)90110-9 .
2. Brondyk WH, McKiernan CJ, Fortner KA, Stabila P, Holz RW, Macara IG . Interaction cloning of Rabin3, a novel protein that associates with the Ras-like GTPase Rab3A . Mol Cell Biol . 15 . 3 . 1137–43 . March 1995 . 7532276 . 230335 . 10.1128/MCB.15.3.1137.
3. Web site: Entrez Gene: RAB3A RAB3A, member RAS oncogene family.
4. Betz A, Thakur P, Junge HJ, Ashery U, Rhee JS, Scheuss V, Rosenmund C, Rettig J, Brose N . Functional interaction of the active zone proteins Munc13-1 and RIM1 in synaptic vesicle priming . Neuron . 30 . 1 . 183–96 . April 2001 . 11343654 . 10.1016/s0896-6273(01)00272-0. 11858/00-001M-0000-0012-F596-C . 155875 . free .
5. Web site: Protein unc-13 homolog A. UniProt.
6. Fukuda M . Distinct Rab binding specificity of Rim1, Rim2, rabphilin, and Noc2. Identification of a critical determinant of Rab3A/Rab27A recognition by Rim2 . J. Biol. Chem. . 278 . 17 . 15373–80 . April 2003 . 12578829 . 10.1074/jbc.M212341200 . free .
7. Ostermeier C, Brunger AT . Structural basis of Rab effector specificity: crystal structure of the small G protein Rab3A complexed with the effector domain of rabphilin-3A . Cell . 96 . 3 . 363–74 . February 1999 . 10025402 . 10.1016/S0092-8674(00)80549-8. 15162326 . free .
8. Weber E, Jilling T, Kirk KL . Distinct functional properties of Rab3A and Rab3B in PC12 neuroendocrine cells . J. Biol. Chem. . 271 . 12 . 6963–71 . March 1996 . 8636125 . 10.1074/jbc.271.12.6963. free .
9. Cremers FP, Armstrong SA, Seabra MC, Brown MS, Goldstein JL . REP-2, a Rab escort protein encoded by the choroideremia-like gene . J. Biol. Chem. . 269 . 3 . 2111–7 . January 1994 . 10.1016/S0021-9258(17)42142-9 . 8294464 . free .
10. Pereira-Leal JB, Strom M, Godfrey RF, Seabra MC . Structural determinants of Rab and Rab Escort Protein interaction: Rab family motifs define a conserved binding surface . Biochem. Biophys. Res. Commun. . 301 . 1 . 92–7 . January 2003 . 12535645 . 10.1016/s0006-291x(02)02963-7.