| Country: | ZAF |
| Route: | 321 |
| Direction A: | North |
| Direction B: | South |
| Type: | R |
| Next Type: | R |
| Previous Type: | R |
| Previous Route: | 320 |
| Next Route: | 322 |
The R321 is a Regional Route in South Africa that connects Grabouw in the south-west to the R45 between Franschhoek and Villiersdorp in the north-east.[1]
From its southern origin at the N2, it runs along the eastern edge of Grabouw heading north-east. It crosses the dam wall of Theewaterskloof Dam after which it immediately ends, reaching the R45.