Precorrin-2 C20-methyltransferase explained

In enzymology, a precorrin-2 C20-methyltransferase is an enzyme that catalyzes the chemical reaction

S-adenosyl-L-methionine + precorrin-2

S-adenosyl-L-homocysteine + precorrin-3AThe two substrates of this enzyme are S-adenosyl methionine and precorrin 2 and its two products are S-adenosylhomocysteine and precorrin 3A.

This enzyme belongs to the family of transferases, specifically those transferring one-carbon group methyltransferases. The systematic name of this enzyme class is S-adenosyl-L-methionine:precorrin-4 C20-methyltransferase and another names in common use is CobI. The enzyme is part of the biosynthetic pathway to cobalamin (vitamin B₁₂) in aerobic bacteria.

See also

• Cobalamin biosynthesis

Structural studies

As of late 2007, two structures have been solved for this class of enzymes, with PDB accession codes and .

References

• Stolowich NJ, Iida K, Scott AI . 1992 . Expression of 9 Salmonella typhimurium enzymes for cobinamide synthesis. Identification of the 11-methyl and 20-methyl transferases of corrin biosynthesis . FEBS Lett. . 301 . 73 - 8 . 1451790 . 10.1016/0014-5793(92)80213-Z . 1 . 20198692 . free . 1992FEBSL.301...73R .
• Anousis N, Stolowich NJ, Holderman MT, Scott AI . 1995 . Overexpression in Escherichia coli of 12 vitamin B12 biosynthetic enzymes . Protein Expr. Purif. . 6 . 155 - 63 . 7606163 . 10.1006/prep.1995.1019 . 2 .
• Debussche L, Thibaut D, Cameron B, Crouzet J, Blanche F . 1993 . Biosynthesis of the corrin macrocycle of coenzyme B12 in Pseudomonas denitrificans . J. Bacteriol. . 175 . 7430 - 40 . 8226690 . 22 . 10.1128/jb.175.22.7430-7440.1993 . 206888 .