PRPF6 explained

Pre-mRNA-processing factor 6 is a protein that in humans is encoded by the PRPF6 gene.^{[1] [2] [3]}

The protein encoded by this gene appears to be involved in pre-mRNA splicing, possibly acting as a bridging factor between U5 and U4/U6 snRNPs in formation of the spliceosome. The encoded protein also can bind androgen receptor, providing a link between transcriptional activation and splicing.

Interactions

PRPF6 has been shown to interact with TXNL4B,^[4] ARAF^{[5] [6]} and Androgen receptor.^[7]

Further reading

• Maruyama K, Sugano S . Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides. . Gene . 138 . 1–2 . 171–4 . 1994 . 8125298 . 10.1016/0378-1119(94)90802-8 .
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, etal . Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library. . Gene . 200 . 1–2 . 149–56 . 1997 . 9373149 . 10.1016/S0378-1119(97)00411-3 .
• Yuryev A, Ono M, Goff SA, etal . Isoform-specific localization of A-RAF in mitochondria. . Mol. Cell. Biol. . 20 . 13 . 4870–8 . 2000 . 10848612 . 10.1128/MCB.20.13.4870-4878.2000. 85938 .
• Deloukas P, Matthews LH, Ashurst J, etal . The DNA sequence and comparative analysis of human chromosome 20. . Nature . 414 . 6866 . 865–71 . 2002 . 11780052 . 10.1038/414865a . 2001Natur.414..865D . free .
• Jurica MS, Licklider LJ, Gygi SR, etal . Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis. . RNA . 8 . 4 . 426–39 . 2002 . 11991638 . 10.1017/S1355838202021088. 1370266 .
• Zhao Y, Goto K, Saitoh M, etal . Activation function-1 domain of androgen receptor contributes to the interaction between subnuclear splicing factor compartment and nuclear receptor compartment. Identification of the p102 U5 small nuclear ribonucleoprotein particle-binding protein as a coactivator for the receptor. . J. Biol. Chem. . 277 . 33 . 30031–9 . 2002 . 12039962 . 10.1074/jbc.M203811200 . free .
• Strausberg RL, Feingold EA, Grouse LH, etal . Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. . Proc. Natl. Acad. Sci. U.S.A. . 99 . 26 . 16899–903 . 2003 . 12477932 . 10.1073/pnas.242603899 . 139241 . 2002PNAS...9916899M . free .
• Yuryev A, Wennogle LP . Novel raf kinase protein-protein interactions found by an exhaustive yeast two-hybrid analysis. . Genomics . 81 . 2 . 112–25 . 2003 . 12620389 . 10.1016/S0888-7543(02)00008-3 .
• Goto K, Zhao Y, Saito M, etal . Activation function-1 domain of androgen receptor contributes to the interaction between two distinct subnuclear compartments. . J. Steroid Biochem. Mol. Biol. . 85 . 2–5 . 201–8 . 2003 . 12943705 . 10.1016/S0960-0760(03)00196-1 . 46683850 .
• Ota T, Suzuki Y, Nishikawa T, etal . Complete sequencing and characterization of 21,243 full-length human cDNAs. . Nat. Genet. . 36 . 1 . 40–5 . 2004 . 14702039 . 10.1038/ng1285 . free .
• Will CL, Schneider C, Hossbach M, etal . The human 18S U11/U12 snRNP contains a set of novel proteins not found in the U2-dependent spliceosome. . RNA . 10 . 6 . 929–41 . 2004 . 15146077 . 10.1261/rna.7320604. 1370585 .
• Sun X, Zhang H, Wang D, etal . DLP, a novel Dim1 family protein implicated in pre-mRNA splicing and cell cycle progression. . J. Biol. Chem. . 279 . 31 . 32839–47 . 2004 . 15161931 . 10.1074/jbc.M402522200 . free .
• Suzuki Y, Yamashita R, Shirota M, etal . Sequence comparison of human and mouse genes reveals a homologous block structure in the promoter regions. . Genome Res. . 14 . 9 . 1711–8 . 2004 . 15342556 . 10.1101/gr.2435604 . 515316 .
• Gerhard DS, Wagner L, Feingold EA, etal . The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). . Genome Res. . 14 . 10B . 2121–7 . 2004 . 15489334 . 10.1101/gr.2596504 . 528928 .
• Laggerbauer B, Liu S, Makarov E, etal . The human U5 snRNP 52K protein (CD2BP2) interacts with U5-102K (hPrp6), a U4/U6.U5 tri-snRNP bridging protein, but dissociates upon tri-snRNP formation. . RNA . 11 . 5 . 598–608 . 2005 . 15840814 . 10.1261/rna.2300805 . 1370748 .
• Batsché E, Yaniv M, Muchardt C . The human SWI/SNF subunit Brm is a regulator of alternative splicing. . Nat. Struct. Mol. Biol. . 13 . 1 . 22–9 . 2006 . 16341228 . 10.1038/nsmb1030 . 11587161 .
• Fan S, Goto K, Chen G, etal . Identification of the functional domains of ANT-1, a novel coactivator of the androgen receptor. . Biochem. Biophys. Res. Commun. . 341 . 1 . 192–201 . 2006 . 16414017 . 10.1016/j.bbrc.2005.12.167 .
• Ewing RM, Chu P, Elisma F, etal . Large-scale mapping of human protein-protein interactions by mass spectrometry. . Mol. Syst. Biol. . 3 . 1. 89 . 2007 . 17353931 . 10.1038/msb4100134 . 1847948 .

External links

• GeneReviews/NCBI/NIH/UW entry on Retinitis Pigmentosa Overview

Notes and References

1. Nishikimi A, Mukai J, Kioka N, Yamada M . A novel mammalian nuclear protein similar to Schizosaccharomyces pombe Prp1p/Zer1p and Saccharomyces cerevisiae Prp6p pre-mRNA splicing factors . Biochim Biophys Acta . 1435 . 1–2 . 147–52 . January 2000 . 10561546 . 10.1016/s0167-4838(99)00203-4.
2. Makarov EM, Makarova OV, Achsel T, Luhrmann R . The human homologue of the yeast splicing factor prp6p contains multiple TPR elements and is stably associated with the U5 snRNP via protein-protein interactions . J Mol Biol . 298 . 4 . 567–75 . June 2000 . 10788320 . 10.1006/jmbi.2000.3685 .
3. Web site: Entrez Gene: PRPF6 PRP6 pre-mRNA processing factor 6 homolog (S. cerevisiae).
4. Sun . Xiaojing . Zhang Hua . Wang Dan . Ma Dalong . Shen Yan . Shang Yongfeng . July 2004 . DLP, a novel Dim1 family protein implicated in pre-mRNA splicing and cell cycle progression . J. Biol. Chem. . 279 . 31 . 32839–47 . United States. 0021-9258. 15161931 . 10.1074/jbc.M402522200 . free .
5. 10.1016/S0888-7543(02)00008-3 . Yuryev . Anton . Wennogle Lawrence P . February 2003 . Novel raf kinase protein-protein interactions found by an exhaustive yeast two-hybrid analysis . Genomics . 81 . 2 . 112–25 . United States. 0888-7543. 12620389 .
6. 10.1128/MCB.20.13.4870-4878.2000 . Yuryev . A . Ono M . Goff S A . Macaluso F . Wennogle L P . July 2000 . Isoform-specific localization of A-RAF in mitochondria . Mol. Cell. Biol. . 20 . 13 . 4870–8 . UNITED STATES. 0270-7306. 10848612 . 85938 .
7. Zhao . Yue . Goto Kiminobu . Saitoh Masayuki . Yanase Toshihiko . Nomura Masatoshi . Okabe Taijiro . Takayanagi Ryoichi . Nawata Hajime . August 2002 . Activation function-1 domain of androgen receptor contributes to the interaction between subnuclear splicing factor compartment and nuclear receptor compartment. Identification of the p102 U5 small nuclear ribonucleoprotein particle-binding protein as a coactivator for the receptor . J. Biol. Chem. . 277 . 33 . 30031–9 . United States. 0021-9258. 12039962 . 10.1074/jbc.M203811200 . free .