NUBP2 explained

Nucleotide-binding protein 2 (NBP 2) also known as cytosolic Fe-S cluster assembly factor NUBP2 is a protein that in humans is encoded by the NUBP2 gene.^[1]

NUBP2 is a member of the NUBP/MRP gene subfamily of ATP-binding proteins. There are two types in eukaryotes NUBP1 and NUBP2, and one novel human gene that define NBP nucleotide-binding proteins (NUBP/MRP-multidrug resistance-associated protein)^[1] in mammalian cells requires the maturation of cytosolic^[2] iron-sulfur (Fe/S)^[3] proteins as Nubp1 is involved in the formation of extramitochondrial Fe/S proteins^[4] the cell division inhibitor MinD is homologous^[5] and involve two proteins components of the (FeS) protein assembly machinery closely similar cytosolic^[4] soluble P loop^[5] NTPase where Nar1^{[6] [7]} is required for assembly,^[8] identified Cfd1p^{[9] [10]} in cytosolic and nuclear Fe/S protein biogenesis^[3] in yeast.^[11] Nubp proteins NTPase Nbp35p.^{[7] [8]} MinD is homologous to members in MinD of E. coli, a relative of the ParA family.^{[5] [12] [13]}

Morphology

^{Further information: Morphology (biology)}

NBP35 bacterial plasmids F (the classical Escherichia coli sex factor)^[5] is found in all nuclear genes in vegetative and gametic flagella of the unicellular green algae C. reinhardtii and nuclear Fe/S protein biogenesis required for cytosolic iron-sulfur protein assembly; MNP =MRP-like; MRP (Multiple Resistance and pH adaptation) MRP/NBP35-like P-loop NTPase similar to; and functions as minD_arch; cell division ATPase MinD, archaeal and homologue's of NUBP1. The NBP35 gene is conserved in archaea^[14] Bacteria, Metazoa, Fungi and other Eukaryotes and with considerable divergence from the yeast; Cfd1-Nbp35 Fe-S to man. In a scaffold complex^[15] protein to form large molecular assemblies that store Fe(III) and 4Fe-4S seen as secondary to defects inactivated to accomplish its functions as physiologically relevant form(s) Fe/S proteins Iron regulatory protein 1 (IRP1) is regulated through^[10] prevents deficiencies and increased mutation rates^[13] that characterized a plant P loop NTPase with sequence similarity to Nbp35 homologue's of NUBP1.^[16]

Interactions

NUBP2 has been shown to interact with...

• ACO1 Iron-responsive element-binding protein 1 (IRE-BP 1) (Iron regulatory protein 1) (IRP1)^{[10] [17]}
• MAPK8IP3 C-jun-amino-terminal kinase-interacting protein 3 (JNK-interacting protein 3) (JIP-3)^[18]
• IGFALS Insulin-like growth factor-binding protein complex acid labile chain precursor (ALS)^{[18] [19]}
• KIF11 Kinesin-like protein KIF11 (Kinesin-related motor protein Eg5)^[20]
• SEPP1 Selenoprotein P precursor (SeP)^[13]
• CA1 Carbonic anhydrase 1 (EC 4.2.1.1) (Carbonic anhydrase I) (Carbonate dehydratase I) (CA-I)^{[12] [13] [16]}

Further reading

• Guey LT, GarcÃa-Closas M, Murta-Nascimento C, etal . Genetic susceptibility to distinct bladder cancer subphenotypes. . Eur. Urol. . 57 . 2 . 283–92 . 2010 . 19692168 . 10.1016/j.eururo.2009.08.001 . 3220186.
• Roy A, Solodovnikova N, Nicholson T, etal . A novel eukaryotic factor for cytosolic Fe-S cluster assembly. . EMBO J. . 22 . 18 . 4826–35 . 2003 . 12970194 . 10.1093/emboj/cdg455 . 212722.
• Gerhard DS, Wagner L, Feingold EA, etal . The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). . Genome Res. . 14 . 10B . 2121–7 . 2004 . 15489334 . 10.1101/gr.2596504 . 528928.
• Ota T, Suzuki Y, Nishikawa T, etal . Complete sequencing and characterization of 21,243 full-length human cDNAs. . Nat. Genet. . 36 . 1 . 40–5 . 2004 . 14702039 . 10.1038/ng1285 . free .
• Bonaldo MF, Lennon G, Soares MB . Normalization and subtraction: two approaches to facilitate gene discovery. . Genome Res. . 6 . 9 . 791–806 . 1996 . 8889548 . 10.1101/gr.6.9.791. free .
• Shen M, Vermeulen R, Rajaraman P, etal . Polymorphisms in innate immunity genes and lung cancer risk in Xuanwei, China. . Environ. Mol. Mutagen. . 50 . 4 . 285–90 . 2009 . 19170196 . 10.1002/em.20452 . 2666781. 2009EnvMM..50..285S .
• Strausberg RL, Feingold EA, Grouse LH, etal . Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. . Proc. Natl. Acad. Sci. U.S.A. . 99 . 26 . 16899–903 . 2002 . 12477932 . 10.1073/pnas.242603899 . 139241. 2002PNAS...9916899M . free .
• Hosgood HD, Menashe I, He X, etal . PTEN identified as important risk factor of chronic obstructive pulmonary disease. . Respir Med . 103 . 12 . 1866–70 . 2009 . 19625176 . 10.1016/j.rmed.2009.06.016 . 2783799.
• Hosgood HD, Menashe I, Shen M, etal . Pathway-based evaluation of 380 candidate genes and lung cancer susceptibility suggests the importance of the cell cycle pathway. . Carcinogenesis . 29 . 10 . 1938–43 . 2008 . 18676680 . 10.1093/carcin/bgn178 . 2722857.

Notes and References

1. Nakashima H, Grahovac MJ, Mazzarella R, etal . Two novel mouse genes--Nubp2, mapped to the t-complex on chromosome 17, and Nubp1, mapped to chromosome 16--establish a new gene family of nucleotide-binding proteins in eukaryotes. . Genomics . 60 . 2 . 152–60 . 1999 . 10486206 . 10.1006/geno.1999.5898 .
2. Netz DJ, Pierik AJ, Stümpfig M, Mühlenhoff U, Lill R . The Cfd1-Nbp35 complex acts as a scaffold for iron-sulfur protein assembly in the yeast cytosol.. Nat Chem Biol . 3 . 5 . 278–86 . 2007 . 17401378 . 10.1038/nchembio872 .
3. Hausmann A, Aguilar Netz DJ, Balk J, Pierik AJ, Mühlenhoff U, Lill R . The eukaryotic P-loop NTPase Nbp35: an essential component of the cytosolic and nuclear iron-sulfur protein assembly machinery.. Proc Natl Acad Sci U S A . 102 . 9 . 3266–71 . 2005 . 15728363 . 10.1073/pnas.0406447102 . 552912. 2005PNAS..102.3266H. free.
4. Stehling O, Netz DJ, Niggemeyer B, etal . Human Nbp35 is essential for both cytosolic iron-sulfur protein assembly and iron homeostasis. . Mol. Cell. Biol. . 28 . 17 . 5517–28 . 2008 . 18573874 . 10.1128/MCB.00545-08 . 2519719.
5. Motallebi-Veshareh M, Rouch DA, Thomas CM . A family of ATPases involved in active partitioning of diverse bacterial plasmids.. Mol. Microbiol. . 4 . 9 . 1455–63 . 1990 . 2149583 . 10.1111/j.1365-2958.1990.tb02056.x . 40829252.
6. Balk J, Aguilar Netz DJ, Tepper K, Pierik AJ, Lill R . The essential WD40 protein Cia1 is involved in a late step of cytosolic and nuclear iron-sulfur protein assembly.. Mol Cell Biol . 25 . 24 . 10833–41 . 2005 . 16314508 . 10.1128/MCB.25.24.10833-10841.2005 . 1316972.
7. Rutherford JC, Ojeda L, Balk J, Mühlenhoff U, Lill R, Winge DR . Activation of the iron regulon by the yeast Aft1/Aft2 transcription factors depends on mitochondrial but not cytosolic iron-sulfur protein biogenesis. . J Biol Chem . 280 . 11 . 10135–40 . 2005 . 15649888 . 10.1074/jbc.M413731200 . free .
8. Balk J, Pierik AJ, Aguilar Netz DJ, Mühlenhoff U, Lill R . Nar1p, a conserved eukaryotic protein with similarity to Fe-only hydrogenases, functions in cytosolic iron-sulphur protein biogenesis. . Biochem Soc Trans . 33 . Pt.1 . 86–9 . 2005 . 15667273 . 10.1042/BST0330086 . 2010-12-23 . 2012-03-12 . https://web.archive.org/web/20120312014201/http://db.yeastgenome.org/cgi-bin/reference/reference.pl?pubmed=15667273 . dead .
9. Yarunin A, Panse VG, Petfalski E, Dez C, Tollervey D, Hurt EC . Functional link between ribosome formation and biogenesis of iron-sulfur proteins.. EMBO J. . 24 . 3 . 580–8 . 2005 . 15660135 . 10.1038/sj.emboj.7600540 . 548649.
10. Roy A, Solodovnikova N, Nicholson T, etal . A novel eukaryotic factor for cytosolic Fe-S cluster assembly. . EMBO J. . 22 . 18 . 4826–35 . 2003 . 12970194 . 10.1093/emboj/cdg455 . 212722.
11. Okuno T, Yamabayashi H, Kogure K . Comparison of intracellular localization of Nubp1 and Nubp2 using GFP fusion proteins.. Mol Biol Rep . 37 . 3 . 1165–8 . 2010 . 19263241 . 10.1007/s11033-009-9477-7 . 998955.
12. Bignell C, Thomas CM . The bacterial ParA-ParB partitioning proteins.. J Biotechnol . 91 . 2 . 1–34 . 2001 . 11522360 . 10.1016/S0168-1656(01)00293-0 . 0168-1656.
13. Fukushima K, Ogawa H, Takahashi K, Naito H, Funayama Y, Kitayama T, Yonezawa H, Sasaki I . Non-pathogenic bacteria modulate colonic epithelial gene expression in germ-free mice.. Scand J Gastroenterol . 38 . 6 . 626–34 . 2003 . 12825871 . 10.1080/00365510310000376. 25927173. 0036-5521 .
14. Kohbushi H, Nakai Y, Kikuchi S, Yabe T, Hori H, Nakai M . Arabidopsis cytosolic Nbp35 homodimer can assemble both [2Fe-2S] and [4Fe-4S] clusters in two distinct domains.. Biochem Biophys Res Commun . 378 . 4 . 810–5 . 2009 . 19084504 . 10.1016/j.bbrc.2008.11.138 .
15. Bych K, Netz DJ, Vigani G, Bill E, Lill R, Pierik AJ, Balk J . The essential cytosolic iron-sulfur protein Nbp35 acts without Cfd1 partner in the green lineage.. J Biol Chem . 283 . 51 . 35797–804 . 2008 . 18957412 . 10.1074/jbc.M807303200 . free . 2318/1655385 . free .
16. Web site: Entrez Gene: UniGene Hs.256549 Nucleotide binding protein 2 (MinD homolog, E. coli) (NUBP2).
17. Web site: Entrez Gene: NUCLEOTIDE-BINDING PROTEIN 2; NUBP2 CYTOSOLIC FE-S CLUSTER DEFICIENT 1, S. CEREVISIAE, HOMOLOG OF; CFD1 Gene map locus: 16p13.3.
18. Kargul GJ, Nagaraja R, Shimada T, Grahovac MJ, Lim MK, Nakashima H, Waeltz P, Ma P, Chen E, Schlessinger D, Ko MS . Eleven densely clustered genes, six of them novel, in 176 kb of mouse t-complex DNA.. Genome Res. . 10 . 7 . 916–23 . 2000 . 10899141 . 10.1101/gr.10.7.916 . 310918.
19. Sano Y, Shimada T, Nakashima H, Nicholson RH, Eliason JF, Kocarek TA, Ko MS . Random monoallelic expression of three genes clustered within 60 kb of mouse t complex genomic DNA.. Genome Res. . 11 . 11 . 1833–41 . 2001 . 11691847 . 10.1101/gr.194301 . 311134.
20. Christodoulou A, Lederer CW, Surrey T, Vernos I, Santama N . Motor protein KIFC5A interacts with Nubp1 and Nubp2, and is implicated in the regulation of centrosome duplication.. J Cell Sci . 119 . Pt 10 . 2035–47 . 2006 . 16638812 . 10.1242/jcs.02922 . 1004740 .