5-(carboxyamino)imidazole ribonucleotide synthase explained

In enzymology, a 5-(carboxyamino)imidazole ribonucleotide synthase is an enzyme that catalyzes the chemical reaction

ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole + HCO₃^-

ADP + phosphate + 5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole

The 3 substrates of this enzyme are ATP, 5-amino-1-(5-phospho-D-ribosyl)imidazole ("AIR"), and HCO₃^-, whereas its 3 products are ADP, phosphate, and 5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole.

This enzyme belongs to the family of ligases, specifically those forming generic carbon-nitrogen bonds. The systematic name of this enzyme class is 5-amino-1-(5-phospho-D-ribosyl)imidazole:carbon-dioxide ligase (ADP-forming).

References

• Meyer E, Leonard NJ, Bhat B, Stubbe J, Smith JM . 1992 . Purification and characterization of the purE, purK, and purC gene products: identification of a previously unrecognized energy requirement in the purine biosynthetic pathway . Biochemistry . 31 . 5022–32 . 1534690 . 10.1021/bi00136a016 . 21 .
• Mueller EJ, Meyer E, Rudolph J, Davisson VJ, Stubbe J . 1994 . N5-carboxyaminoimidazole ribonucleotide: evidence for a new intermediate and two new enzymatic activities in the de novo purine biosynthetic pathway of Escherichia coli . Biochemistry . 33 . 2269–78 . 8117684 . 10.1021/bi00174a038 . 8 .
• Thoden JB, Kappock TJ, Stubbe J, Holden HM . 1999 . Three-dimensional structure of N5-carboxyaminoimidazole ribonucleotide synthetase: a member of the ATP grasp protein superfamily . Biochemistry . 38 . 15480–92 . 10569930 . 10.1021/bi991618s . 47 .