| Musical Theatre Karlín | |
| Native Name: | Hudební divadlo Karlín |
| Native Name Lang: | Czech |
| Address: | Křižíkova 283, Karlín |
| City: | Prague |
| Country: | Czech Republic |
| Coordinates: | 50.0901°N 14.4412°W |
| Type: | Theatre |
| Currentuse: | Musical theatre |
| Built: | 1881 |
The Musical Theatre Karlín (Czech: Hudební divadlo Karlín) is a theatre in the Karlín district of Prague devoted largely to the performance of operettas and musical theatre. Built in 1881, it is now the second largest theatre in Prague after the Prague State Opera.