Mir-744 microRNA precursor family explained

In molecular biology mir-744 microRNA is a short RNA molecule. MicroRNAs function to regulate the expression levels of other genes by several mechanisms.

miR-744 and cancer in mice

miR-744 plays a role in tumour development and growth in mouse cell lines. Its expression induces cyclin B1 expression, whilst knockdown sees a resultant decreased level of mouse cyclin B through the Ccnb1 gene.^[1] Short-term overexpression of miR-744 in mouse cell lines has been seen to enhance cell proliferation, whilst chromosomal instability and in vivo suppression are concurrent with a prolonged expression.

TGF-β1 repression

Multiple miR-744 binding sites have been identified in the proximal 3' untranslated region of transforming growth factor beta 1 (TGF-β1). Direct targeting of TGF-β1 by miR-744 has been identified, and transfection is seen to inhibit endogenous TGF-β1 synthesis by directing post-transcriptional regulation.^[2]

EEF1A2 repression

miR-744 directly targets translation elongation factor and known protooncogene EEF1A2.^[3] mIR-744 also upregulates during resveratrol treatment of MCF7 breast cancer cells.^[4]

See also

• MicroRNA

Further reading

• Song . M. Y. . Pan . K. F. . Su . H. J. . Zhang . L. . Ma . J. L. . Li . J. Y. . Yuasa . Y. . Kang . D. . Kim . Y. S. . You . W. C. . Califano . Joseph . Identification of Serum MicroRNAs as Novel Non-Invasive Biomarkers for Early Detection of Gastric Cancer . 10.1371/journal.pone.0033608 . PLOS ONE . 7 . 3 . e33608 . 2012 . 22432036 . 3303856 . 2012PLoSO...733608S . free .
• Mi . Q. S. . Weiland . M. . Qi . R. Q. . Gao . X. H. . Poisson . L. M. . Zhou . L. . Homberg . Judith . Identification of Mouse Serum miRNA Endogenous References by Global Gene Expression Profiles . 10.1371/journal.pone.0031278 . PLOS ONE . 7 . 2 . e31278 . 2012 . 22348064 . 3277497 . 2012PLoSO...731278M . free .
• Chen . Y. Q. . Wang . X. X. . Yao . X. M. . Zhang . D. L. . Yang . X. F. . Tian . S. F. . Wang . N. S. . 10.5301/jn.5000034 . Abated microRNA-195 expression protected mesangial cells from apoptosis in early diabetic renal injury in mice . Journal of Nephrology . 25 . 4 . 566–576 . 2012 . 21983986 .
• Yoke-Kqueen . C. . Yoke-Kqueen . C. . Sabariah . A. R. . Shiran . M. S. . Singh . A. . Learn-Han . L. . Differential microRNA expression and identification of putative miRNA targets and pathways in head and neck cancers . 10.3892/ijmm.2011.714 . International Journal of Molecular Medicine . 28 . 3 . 327–336 . 2011 . 21637912 . free .

Notes and References

1. Huang V, Place RF, Portnoy V, Wang J, Qi Z, Jia Z, etal . Upregulation of Cyclin B1 by miRNA and its implications in cancer. . Nucleic Acids Res . 2012 . 40 . 4 . 1695–707 . 22053081 . 10.1093/nar/gkr934 . 3287204 .
2. Martin J, Jenkins RH, Bennagi R, Krupa A, Phillips AO, Bowen T, etal . Post-transcriptional regulation of Transforming Growth Factor Beta-1 by microRNA-744. . PLOS ONE . 2011 . 6 . 10 . e25044 . 21991303 . 10.1371/journal.pone.0025044 . 3186795 . 2011PLoSO...625044M . free .
3. Vislovukh. A. Kratassiouk. G. Porto. E. Gralievska. N. Beldiman. C. Pinna. G. El'skaya. A. Harel-Bellan. A. Negrutskii. B. Groisman. I. Proto-oncogenic isoform A2 of eukaryotic translation elongation factor eEF1 is a target of miR-663 and miR-744.. British Journal of Cancer. 11 June 2013. 108. 11. 2304–11. 23695020. 10.1038/bjc.2013.243. 3681015.
4. Vislovukh. A. Kratassiouk. G. Porto. E. Gralievska. N. Beldiman. C. Pinna. G. El'skaya. A. Harel-Bellan. A. Negrutskii. B. Groisman. I. Proto-oncogenic isoform A2 of eukaryotic translation elongation factor eEF1 is a target of miR-663 and miR-744.. British Journal of Cancer. 11 June 2013. 108. 11. 2304–11. 23695020. 10.1038/bjc.2013.243. 3681015.