Methanosarcina barkeri explained

Methanosarcina barkeri is the type species of the genus Methanosarcina, characterized by its wide range of substrates used in methanogenesis. While most known methanogens produce methane from H2 and CO2, M. barkeri can also dismutate methylated compounds such as methanol or methylamines, oxidize acetate, and reduce methylated compounds with H2. This makes M. barkeri one of the few Methanosarcina species capable of utilizing all four known methanogenesis pathways.[1] Even among other Methanosarcinales, which commonly utilize a broad range of substrates, the ability to grow on H2 and CO2 is rare due to the requirement for high H2 partial pressure.[2] [3] Like other Methanosarcina species, M. barkeri has a large genome (4.53 Mbp for the type strain MS, 4.9 Mbp for the Wiesmoor strain, and 4.5 Mbp for the CM2 strain), although it is significantly smaller than the largest archaeal genome of Methanosarcina acetivorans (5.75 Mbp for the type strain C2A).[4] [5] [6] It is also one of the few archaea, particularly among anaerobic species, that is genetically tractable and can be used for genetic studies.[7] [8] [9]

Isolation and Physiology

The type strain of the species designated as MS (DSM 800, JCM 10043, ATCC 43569) was isolated from the sewage digester.[10] It was designated as neotype of the species because the original type strain of M. barkeri described in the PhD thesis of Schnellen in 1947 was lost. Besides the type strain MS, a few more strains of M. barkeri described. For example, the strain Fusaro is commonly used for genetic studies of Methanosarcina.[11] It was isolated in mud samples taken from Lake Fusaro, a freshwater lake near Naples.[12] As other Methanosarcina species, M. barkeri can also be found in marine and freshwater sediments, sewage, soil, and landfills.

Morphology of Methanosarcina cells depends on growing conditions, e.g. on salt concentrations.[13] M. barkeri shows this variable morphology: when grown in freshwater medium, these microbes grow into large, multicellular aggregates embedded in a matrix of methanochondroitin, while growing in marine environment as single, irregular cocci, only surrounded by the S-layer, but no methanochondroitin.[14] The aggregates can grow large enough to be seen by the naked eye.[15] Methanosarcina could produce positive Gram stain, but generally, it is Gram variable. M. barkeri has a thick cell wall compounded by a short lipid cell membrane that is similar in structure to most other methanogens. However, its cell walls do not contain peptidoglycan.[16] M. barkeri str. fusaro has no flagellum but has potential for movement through the creation of gas vesicles. These gas vesicles have only been produced in the presence of hydrogen and carbon dioxide, likely acting as a response to a hydrogen gradient.M. barkeris chromosome is large and circular, derived from its remarkable ability to metabolize a variety of different carbon molecules. This offers the species an advantage as though it is immotile, it can adapt to its environment depending on the energy sources available. M. barkeris circular plasmid consists of about twenty genes.

Applications and importance

Methanosarcina barkeris unique nature as an anaerobic methanogen that ferments many carbon sources can have many implications for future biotechnology and environmental studies.[17] As M. barkeri is found in the rumen of cows, a place with an extreme dearth of oxygen, it is classified as an extreme anaerobe.[18] Furthermore, the methane gas produced by cows due to M. barkeri could play a role in greenhouse gas production.[18] However, since M. barkeri can survive in extreme conditions and produce methane, M. barkeri can be implemented in low pH ecosystems, effectively neutralizing the acidity environment, and making it more amenable for other methanogens.[18] This, in turn, would allow people to harness the pure methane produced at landfills or through cow waste.[18] Evidently, the implications of M. barkeri are those aligned with potential alternative energy and investment.[18]

Further reading

External links

Notes and References

  1. Mand . Thomas D. . Metcalf . William W. . 2019-11-20 . Energy Conservation and Hydrogenase Function in Methanogenic Archaea, in Particular the GenusMethanosarcina . Microbiology and Molecular Biology Reviews . 83 . 4 . 10.1128/mmbr.00020-19 . 31533962 . 6759668 . 1092-2172.
  2. Thauer . Rudolf K. . Kaster . Anne-Kristin . Seedorf . Henning . Buckel . Wolfgang . Hedderich . Reiner . 2008-06-30 . Methanogenic archaea: ecologically relevant differences in energy conservation . Nature Reviews Microbiology . 6 . 8 . 579–591 . 10.1038/nrmicro1931 . 1740-1526.
  3. Feldewert . Christopher . Lang . Kristina . Brune . Andreas . 2020-08-21 . The hydrogen threshold of obligately methyl-reducing methanogens . FEMS Microbiology Letters . 367 . 17 . 10.1093/femsle/fnaa137 . 32821944 . 7485788 . 1574-6968.
  4. Maeder . Dennis L. . Anderson . Iain . Brettin . Thomas S. . Bruce . David C. . Gilna . Paul . Han . Cliff S. . Lapidus . Alla . Metcalf . William W. . Saunders . Elizabeth . Tapia . Roxanne . Sowers . Kevin R. . 2006-11-15 . The Methanosarcina barkeri Genome: Comparative Analysis with Methanosarcina acetivorans and Methanosarcina mazei Reveals Extensive Rearrangement within Methanosarcinal Genomes . Journal of Bacteriology . 188 . 22 . 7922–7931 . 10.1128/jb.00810-06 . 16980466 . 1636319 . 0021-9193.
  5. Web site: DasSarma . Shiladitya . 2002-05-28 . Faculty Opinions recommendation of The genome of M. acetivorans reveals extensive metabolic and physiological diversity. . 10.3410/f.1006490.81806 . free .
  6. Protasov . Evgenii . Reeh . Hanna . Liu . Pengfei . Poehlein . Anja . Platt . Katja . Heimerl . Thomas . Hervé . Vincent . Daniel . Rolf . Brune . Andreas . 2024-08-06 . Genome reduction in novel, obligately methyl-reducing Methanosarcinales isolated from arthropod guts (Methanolapillus gen. nov. and Methanimicrococcus) . FEMS Microbiology Ecology . 100 . 9 . 10.1093/femsec/fiae111 . 1574-6941.
  7. Gonnerman . Matthew C. . Benedict . Matthew N. . Feist . Adam M. . Metcalf . William W. . Price . Nathan D. . 2013-03-26 . Genomically and biochemically accurate metabolic reconstruction of Methanosarcina barkeri Fusaro, iMG746 . Biotechnology Journal . 8 . 9 . 1070–1079 . 10.1002/biot.201200266 . 23420771 . 1860-6768.
  8. Metcalf . William W. . Zhang . Jun Kai . Apolinario . Ethel . Sowers . Kevin R. . Wolfe . Ralph S. . 1997-03-18 . A genetic system for Archaea of the genus Methanosarcina : Liposome-mediated transformation and construction of shuttle vectors . Proceedings of the National Academy of Sciences . 94 . 6 . 2626–2631 . 10.1073/pnas.94.6.2626 . free . 9122246 . 20139 . 0027-8424.
  9. Boccazzi . Paolo . Zhang . Jun Kai . Metcalf . William W. . May 2000 . Generation of Dominant Selectable Markers for Resistance to Pseudomonic Acid by Cloning and Mutagenesis of the ileS Gene from the Archaeon Methanosarcina barkeri Fusaro . Journal of Bacteriology . 182 . 9 . 2611–2618 . 10.1128/jb.182.9.2611-2618.2000 . 10762266 . 111328 . 0021-9193.
  10. Balch . W E . Fox . G E . Magrum . L J . Woese . C R . Wolfe . R S . 1979 . Methanogens: reevaluation of a unique biological group. . Microbiological Reviews . 43 . 2 . 260–296 . 10.1128/mmbr.43.2.260-296.1979 . 0146-0749.
  11. Web site: Escalante-Semerena . Jorge . 2003-01-02 . Faculty Opinions recommendation of Genetic analysis of the archaeon Methanosarcina barkeri Fusaro reveals a central role for Ech hydrogenase and ferredoxin in methanogenesis and carbon fixation. . 10.3410/f.1010824.177471 . free .
  12. Web site: Brill . Jessica . Methanosarcina barkeri Fusaro, DSM 804 . dead . https://web.archive.org/web/20150713055321/http://genome.jgi.doe.gov/metba/metba.home.html . 13 July 2015 . 2 June 2014.
  13. Sowers. K. R.. Boone. J. E.. Gunsalus. R. P.. 1993. Disaggregation of Methanosarcina spp. and Growth as Single Cells at Elevated Osmolarity. Applied and Environmental Microbiology. 59. 11. 3832–3839. 0099-2240. 182538. 16349092. 10.1128/AEM.59.11.3832-3839.1993.
  14. Maeder. Dennis. Anderson. Iian. November 2006. The Methanosarcina barkeri Genome: Comparative Analysis with Methanosarcina acetivorans and Methanosarcina mazei Reveals Extensive Rearrangement within Methanosarcinal Genomes. Journal of Bacteriology. 188. 22. 7922–7931. 10.1128/JB.00810-06. 1636319. 16980466.
  15. Balch. W. E.. Fox. G. E.. Magrum. L. J.. Woese. C. R.. Wolfe. R. S.. 1979. Methanogens: reevaluation of a unique biological group. Microbiological Reviews. 43. 2. 260–296. 0146-0749. 281474. 390357. 10.1128/MMBR.43.2.260-296.1979.
  16. Kandler . Otto . Hippe . Hans . Lack of peptidoglycan in the cell walls of Methanosarcina barkeri . Archives of Microbiology . 113 . 1–2 . 1977 . 57–60 . 10.1007/bf00428580 . 889387 . 19145374 .
  17. Balch . W.E. . 1979 . Methanogens:reevaluation of a unique biological group . Microbiology and Molecular Biology Reviews . 43 . 2 . 260–96 . 10.1128/mmbr.43.2.260-296.1979 . 281474 . 390357.
  18. Hook . Sarah . McBride . Brian. December 2010. Methanogens: Methane Producers of the Rumen and Mitigation Strategies . Archaea. 2010. 11. 3021854 . 21253540 . 10.1155/2010/945785 . free .

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