List of restriction enzyme cutting sites: S explained

See main article: List of restriction enzyme cutting sites and Restriction enzyme.

This article contains a list of the most studied restriction enzymes whose names start with S. It contains approximately 130 enzymes.The following information is given:

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Restriction enzymes

S

Enzyme PDB code Source Recognition sequence Cut Isoschizomers

SacI^[1] Streptomyces achromogenes 5' GAGCTC
3' CTCGAG 5' ---GAGCT {{pad|2em}} C--- 3'
3' ---C {{pad|2em}} TCGAG--- 5'

SacII Streptomyces achromogenes 5' CCGCGG
3' GGCGCC 5' ---CCGC {{pad|2em}} GG--- 3'
3' ---GG {{pad|2em}} CGCC--- 5' Cfr42I

SacNI Streptomyces achromogenes N-J-H 5' GRGCYC
3' CYCGRG 5' ---GRGCY {{pad|2em}} C--- 3'
3' ---C {{pad|2em}} YCGRG--- 5'

SalI Streptomyces albus 5' GTCGAC
3' CAGCTG 5' ---G {{pad|2em}} TCGAC--- 3'
3' ---CAGCT {{pad|2em}} G--- 5'

SalPI Streptomyces albus 5' CTGCAG
3' GACGTC 5' ---CTGCA {{pad|2em}} G--- 3'
3' ---G {{pad|2em}} ACGTC--- 5' ApiI, BspMAI, CstI, Ecl37kI, Ecl2zI, Psp23I, PstI, Srl5DI, YenI

SanDI Streptomyces sp. 5' GGGWCCC
3' CCCWGGG 5' ---GG {{pad|2em}} GWCCC--- 3'
3' ---CCCWG {{pad|2em}} GG--- 5'

SapI Saccharopolyspora sp. 5' GCTCTTC
3' CGAGAAG 5' ---GCTCTTCN {{pad|2em}} NNN--- 3'
3' ---CGAGAAGNNNN {{pad|2em}} --- 5'

SarI Streptomyces aureomonopodiales 5' AGGCCT
3' TCCGGA 5' ---AGG {{pad|2em}} CCT--- 3'
3' ---TCC {{pad|2em}} GGA--- 5' AatI, AspMI, Eco147I, GdiI, PceI, Pme55I, Sru30DI, SseBI, SteI, StuI

SatI Staphylococcus arlettae RFL832 5' GCNGC
3' CGNCG 5' ---GC {{pad|2em}} NGC--- 3'
3' ---CGN {{pad|2em}} CG--- 5'

SauI Streptomyces aureofaciens IKA 18/4 5' CCTNAGG
3' GGANTCC 5' ---CC {{pad|2em}} TNAGG--- 3'
3' ---GGANT {{pad|2em}} CC--- 5' AocI, BliHKI, BspR7I, CvnI, Lmu60I, OxaNI, SauI, SshAI

Sau96I Staphylococcus aureus PS96 5' GGNCC
3' CCNGG 5' ---G {{pad|2em}} GNCC--- 3'
3' ---CCNG {{pad|2em}} G--- 5' AsuI, Bal228I, Bce22I, BsiZI, BspF4I, Cfr13I, Nsp7121I, UnbI

Sau3239I Streptomyces aureofaciens 5' CTCGAG
3' GAGCTC 5' ---C {{pad|2em}} TCGAG--- 3'
3' ---GAGCT {{pad|2em}} C--- 5' BspAAI, BstVI, MlaAI, PaeR7I, Sbi68I, Sfr274I, SlaI, XpaI

Sau3AI 2REU Staphylococcus aureus 3A 5' GATC
3' CTAG 5' --- {{pad|2em}} GATC--- 3'
3' ---CTAG {{pad|2em}} --- 5' Bce243I, Bsp143I, BspJI, BstMBI, CviAI, Kzo9I, NdeII, Sth368I

SauBMKI Streptomyces aureofaciens BM-K 5' GCCGGC
3' CGGCCG 5' ---GCC {{pad|2em}} GGC--- 3'
3' ---CGG {{pad|2em}} CCG--- 5'

SauHPI Streptomyces aureofaciens R8/26 5' GCCGGC
3' CGGCCG 5' ---GCC {{pad|2em}} GGC--- 3'
3' ---CGG {{pad|2em}} CCG--- 5'

SauLPI Streptomyces aureofaciens B-96 5' GCCGGC
3' CGGCCG 5' ---GCC {{pad|2em}} GGC--- 3'
3' ---CGG {{pad|2em}} CCG--- 5'

SauLPII Streptomyces aureofaciens B-96 5' CTCGAG
3' GAGCTC 5' ---C {{pad|2em}} TCGAG--- 3'
3' ---GAGCT {{pad|2em}} C--- 5' BspAAI, MavI, MlaAI, PaeR7I, Sbi68I, Sfr274I, SlaI, XpaI

SauMI Staphylococcus aureus M 5' GATC
3' CTAG 5' --- {{pad|2em}} GATC--- 3'
3' ---CTAG {{pad|2em}} --- 5' BscFI, BspAI, BstKTI, ChaI, HacI, MkrAI, RalF40I, SsiAI

SauNI Streptomyces aureofaciens NMU 5' GCCGGC
3' CGGCCG 5' ---GCC {{pad|2em}} GGC--- 3'
3' ---CGG {{pad|2em}} CCG--- 5'

SauSI Streptomyces aureofaciens 16 5' GCCGGC
3' CGGCCG 5' ---GCC {{pad|2em}} GGC--- 3'
3' ---CGG {{pad|2em}} CCG--- 5'

SbfI Streptomyces sp. BF-61 5' CCTGCAGG
3' GGACGTCC 5' ---CCTGCA {{pad|2em}} GG--- 3'
3' ---GG {{pad|2em}} ACGTCC--- 5'

Sbi68I Streptomyces bikiniensis JAM68 5' CTCGAG
3' GAGCTC 5' ---C {{pad|2em}} TCGAG--- 3'
3' ---GAGCT {{pad|2em}} C--- 5' BspAAI, MavI, MlaAI, PaeR7I, Sbi68I, Sol10179I, StrI, XpaI

Sbo13I Shigella boydii 13 5' TCGCGA
3' AGCGCT 5' ---TCG {{pad|2em}} CGA--- 3'
3' ---AGC {{pad|2em}} GCT--- 5'

SbvI Streptococcus bovis II/1 5' GGCC
3' CCGG 5' ---GG {{pad|2em}} CC--- 3'
3' ---CC {{pad|2em}} GG--- 5'

ScaI

Streptomyces caespitosus

5' AGTACT
3' TCATGA

5' ---AGT {{pad|2em}} ACT--- 3'
3' ---TCA {{pad|2em}} TGA--- 5'

Acc113I, AssI,

BmcAI, Bpa34I,

DpaI, Eco255I, RflFII, ZrmI

SceIII Saccharomyces cerevisiae 5' GCCGGC
3' CGGCCG 5' ---G {{pad|2em}} CCGGC--- 3'
3' ---CGGCC {{pad|2em}} G--- 5'

SchI Staphylococcus cohnii Lki 19-320 5' GAGTC
3' CTCAG 5' ---GAGTCN4N {{pad|2em}} --- 3'
3' ---CTCAGN4N {{pad|2em}} --- 5'

SchZI Streptomyces chusanensis ZS-2 5' CCGCGG
3' GGCGCC 5' ---CCGC {{pad|2em}} GG--- 3'
3' ---GG {{pad|2em}} CGCC--- 5'

SciI Streptoverticillium cinnamoneum 5' CTCGAG
3' GAGCTC 5' ---CTC {{pad|2em}} GAG--- 3'
3' ---GAG {{pad|2em}} CTC--- 5' BspAAI, BstVI, MlaAI, PaeR7I, SauLPII, SlaI, Sol10179I, XhoI

SciNI Spiroplasma citri ASP2 5' GCGC
3' CGCG 5' ---G {{pad|2em}} CGC--- 3'
3' ---CGC {{pad|2em}} G--- 5' BspLAI, BstHHI, CfoI, FnuDIII, HhaI, Hin6I, HinP1I, HsoI, HspAI

ScrFI Streptococcus cremoris F 5' CCNGG
3' GGNCC 5' ---CC {{pad|2em}} NGG--- 3'
3' ---GGN {{pad|2em}} CC--- 5'

SdaI 2IXS Streptomyces diastaticus Ng7-324 5' CCTGCAGG
3' GGACGTCC 5' ---CCTGCA {{pad|2em}} GG--- 3'
3' ---GG {{pad|2em}} ACGTCC--- 5'

SdiI Streptomyces diastaticus 5' GGCCN5GGCC
3' CCGGN5CCGG 5' ---GGCCNNNN {{pad|2em}} NGGCC--- 3'
3' ---CCGGN {{pad|2em}} NNNNCCGG--- 5'

SduI Streptococcus durans RFL3 5' GDGCHC
3' CHCGDG 5' ---GDGCH {{pad|2em}} C--- 3'
3' ---C {{pad|2em}} HCGDG--- 5' AocII, BmyI, BsoCI, Bsp1286I, BspLS2I, MhlI, NspII

SecI Synechocystis sp. 6701 5' CCNNGG
3' GGNNCC 5' ---C {{pad|2em}} CNNGG--- 3'
3' ---GGNNC {{pad|2em}} C--- 5'

SelI Synechococcus elongatus 5' CGCG
3' GCGC 5' --- {{pad|2em}} CGCG--- 3'
3' ---GCGC {{pad|2em}} --- 5' AccII, Bsh1236I, Bsp123I, Bsp50I, BstFNI, BstUI, BtkI, Csp68KVI

SenPT16I Salmonella enteritidis PT16 5' CGGCCG
3' GCCGGC 5' ---C {{pad|2em}} GGCCG--- 3'
3' ---GCCGG {{pad|2em}} C--- 5' AaaI, BseX3I, BstZI, EagI, EclXI, Eco52I, XmaIII

SenPT14bI Salmonella enteritidis PT14b 5' CCGCGG
3' GGCGCC 5' ---CCGC {{pad|2em}} GG--- 3'
3' ---GG {{pad|2em}} CGCC--- 5'

SepI Staphylococcus epidermidis 5' ATGCAT
3' TACGTA 5' ---ATGCA {{pad|2em}} T--- 3'
3' ---T {{pad|2em}} ACGTA--- 5' BfrBI, Csp68KIII, Mph1103I, NsiI, PinBI, Ppu10I, SspD5II, Zsp2I

SexAI Streptomyces exfoliatus 5' ACCWGGT
3' TGGWCCA 5' ---A {{pad|2em}} CCWGGT--- 3'
3' ---TGGWCC {{pad|2em}} A--- 5'

SexBI Streptomyces exfoliatus 5' CCGCGG
3' GGCGCC 5' ---CCGC {{pad|2em}} GG--- 3'
3' ---GG {{pad|2em}} CGCC--- 5'

SexCI Streptomyces exfoliatus 5' CCGCGG
3' GGCGCC 5' ---CCGC {{pad|2em}} GG--- 3'
3' ---GG {{pad|2em}} CGCC--- 5'

SfaI Streptococcus faecalis zymogenes 5' GGCC
3' CCGG 5' ---GG {{pad|2em}} CC--- 3'
3' ---CC {{pad|2em}} GG--- 5'

SfaNI Streptococcus faecalis ND547 5' GCATC
3' CGTAG 5' ---GCATCN4N {{pad|2em}} NNNN--- 3'
3' ---CGTAGN4NNNNN {{pad|2em}} --- 5'

SfcI Streptococcus faecium 5' CTRYAG
3' GAYRTC 5' ---C {{pad|2em}} TRYAG--- 3'
3' ---GAYRT {{pad|2em}} C--- 5'

SfeI Streptococcus faecalis 5' CTRYAG
3' GAYRTC 5' ---C {{pad|2em}} TRYAG--- 3'
3' ---GAYRT {{pad|2em}} C--- 5'

SfiI 2F03 Streptomyces fimbriatus 5' GGCCN5GGCC
3' CCGGN5CCGG 5' ---GGCCNNNN {{pad|2em}} NGGCC--- 3'
3' ---CCGGN {{pad|2em}} NNNNCCGG--- 5'

SflI Streptoverticillium flavopersicum 5' CTGCAG
3' GACGTC 5' ---CTGCA {{pad|2em}} G--- 3'
3' ---G {{pad|2em}} ACGTC--- 5' ApiI, BspMAI, BsuBI, CfrA4I, Psp23I, PstI, Sag23I, Srl5DI, YenI

SfoI Serratia fonticola 5' GGCGCC
3' CCGCGG 5' ---GGC {{pad|2em}} GCC--- 3'
3' ---CCG {{pad|2em}} CGG--- 5'

Sfr274I Streptomyces fradiae 274 5' CTCGAG
3' GAGCTC 5' ---C {{pad|2em}} TCGAG--- 3'
3' ---GAGCT {{pad|2em}} C--- 5' AbrI, BluI, BssHI, MavI, Sau3239I, Sol10179I, StrI, TliI

Sfr303I Streptomyces fradiae 303 5' CCGCGG
3' GGCGCC 5' ---CCGC {{pad|2em}} GG--- 3'
3' ---GG {{pad|2em}} CGCC--- 5'

SfuI Streptomyces fulvissimus 5' TTCGAA
3' AAGCTT 5' ---TT {{pad|2em}} CGAA--- 3'
3' ---AAGC {{pad|2em}} TT--- 5' AsuII, BimI, Bsp119I, CbiI, Csp68KII, PlaII, Ssp1I, SviI

SgfI

Streptomyces griseoruber

5' GCGATCGC
3' CGCTAGCG

5' ---GCGAT {{pad|2em}} CGC--- 3'
3' ---CGC {{pad|2em}} TAGCG--- 5'

AsiSI,

RgaI, SfaAI

SgrAI 3DPG 3N7B 3N78 3MGY 3MG6 Streptomyces griseus 5' CRCCGGYG
3' GYGGCCRC 5' ---CR {{pad|2em}} CCGGYG--- 3'
3' ---GYGGCC {{pad|2em}} RC--- 5'

SgrBI Streptomyces griseus 5' CCGCGG
3' GGCGCC 5' ---CCGC {{pad|2em}} GG--- 3'
3' ---GG {{pad|2em}} CGCC--- 5'

SimI Staphylococcus intermedius 6H 5' GGGTC
3' CCCAG 5' ---GG {{pad|2em}} GTC--- 3'
3' ---CCCAG {{pad|2em}} --- 5'

SinI Salmonella infantis 5' GGWCC
3' CCWGG 5' ---G {{pad|2em}} GWCC--- 3'
3' ---CCWG {{pad|2em}} G--- 5' Bme216I, CauI, EagMI, FdiI, HgiBI, HgiHIII, Psp03I, VpaK11BI

SlaI Streptomyces lavendulae 5' CTCGAG
3' GAGCTC 5' ---C {{pad|2em}} TCGAG--- 3'
3' ---GAGCT {{pad|2em}} C--- 5' AbrI, BluI, BssHI, MavI, Sau3239I, Sfr274I, TliI, XhoI

SleI Synechococcus leopoliensis 5' CCWGG
3' GGWCC 5' --- {{pad|2em}} CCWGG--- 3'
3' ---GGWCC {{pad|2em}} --- 5' AeuI, AglI, ApaORI, BspNI, Bst2I, EcoRII, SniI, SslI, SspAI, ZanI

Slu1777I Streptomyces lusitanus 1777 5' GCCGGC
3' CGGCCG 5' ---GCC {{pad|2em}} GGC--- 3'
3' ---CGG {{pad|2em}} CCG--- 5'

SmaI Serratia marcescens Sb 5' CCCGGG
3' GGGCCC 5' ---CCC {{pad|2em}} GGG--- 3'
3' ---GGG {{pad|2em}} CCC--- 5' AhyI, CfrJ4I, EaeAI, EclRI, Pac25I, PspAI, TspMI, XcyI, XmaI, XmaCI

SmiI Streptococcus milleri S 5' ATTTAAAT
3' TAAATTTA 5' ---ATTT {{pad|2em}} AAAT--- 3'
3' ---TAAA {{pad|2em}} TTTA--- 5'

SmiMI Sphingobacterium mizutae M 5' CAYN4RTG
3' GTRN4YAC 5' ---CAYNN {{pad|2em}} NNRTG--- 3'
3' ---GTRNN {{pad|2em}} NNYAC--- 5'

SmlI Stenotrophomonas maltophilia 5' CTYRAG
3' GARYTC 5' ---C {{pad|2em}} TYRAG--- 3'
3' ---GARYT {{pad|2em}} C--- 5'

SmuI Sphingobacterium multivorum RFL21 5' CCCGC
3' GGGCG 5' ---CCCGCNNNN {{pad|2em}} NN--- 3'
3' ---GGGCGNNNNNN {{pad|2em}} --- 5'

SmuEI Streptococcus mutans E 5' GGWCC
3' CCWGG 5' ---G {{pad|2em}} GWCC--- 3'
3' ---CCWG {{pad|2em}} G--- 5' Bme216I, CauI, EagMI, FdiI, HgiBI, HgiHIII, Psp03I, VpaK11BI

SnaBI Sphaerotilus natans 5' TACGTA
3' ATGCAT 5' ---TAC {{pad|2em}} GTA--- 3'
3' ---ATG {{pad|2em}} CAT--- 5'

SniI Streptomyces niveus 5' CCWGG
3' GGWCC 5' ---CC {{pad|2em}} WGG--- 3'
3' ---GGW {{pad|2em}} CC--- 5' ApaORI, BseBI, BshGI, BstNI, BstOI, Bst2UI, BthEI, MvaI, SspAI

SnoI Streptomyces novocastria 5' GTGCAC
3' CACGTG 5' ---G {{pad|2em}} TGCAC--- 3'
3' ---CACGT {{pad|2em}} G--- 5' Alw44I, ApaLI, VneI

SolI Streptoverticillium olivoverticillatum 5' GGATCC
3' CCTAGG 5' ---G {{pad|2em}} GATCC--- 3'
3' ---CCTAG {{pad|2em}} G--- 5' AccEBI, AliI, ApaCI, BamHI, Bce751I, CelI, OkrAI, SolI, SurI

Sol10179I Streptomyces olivochromogenes ST10179 5' CTCGAG
3' GAGCTC 5' ---C {{pad|2em}} TCGAG--- 3'
3' ---GAGCT {{pad|2em}} C--- 5' BspAAI, MavI, MlaAI, PaeR7I, Sbi68I, Sol10179I, StrI, XpaI

SpaHI Synechococus parietina 5' GCATGC
3' CGTACG 5' ---GCATG {{pad|2em}} C--- 3'
3' ---C {{pad|2em}} GTACG--- 5'

SpeI Sphaerotilus natans 5' ACTAGT
3' TGATCA 5' ---A {{pad|2em}} CTAGT--- 3'
3' ---TGATC {{pad|2em}} A--- 5' AhII, AclNI, BcuI

SphI Streptomyces phaeochromogenes 5' GCATGC
3' CGTACG 5' ---GCATG {{pad|2em}} C--- 3'
3' ---C {{pad|2em}} GTACG--- 5'

SplI Spirulina platensis 5' CGTACG
3' GCATGC 5' ---C {{pad|2em}} GTACG--- 3'
3' ---GCATG {{pad|2em}} C--- 5'

SpmI Sphingobacterium multivorum RFL21 5' ATCGAT
3' TAGCTA 5' ---AT {{pad|2em}} CGAT--- 3'
3' ---TAGC {{pad|2em}} TA--- 5' BanIII, BbvAII, BdiI, BscI, Bsa29I, BspJI, ClaI, LcaI, PgaI, Ssp27144I

SpoI Salmonella potsdam 5' TCGCGA
3' AGCGCT 5' ---TCG {{pad|2em}} CGA--- 3'
3' ---AGC {{pad|2em}} GCT--- 5'

SpuI Streptomyces pulveraceus 5' CCGCGG
3' GGCGCC 5' ---CCGC {{pad|2em}} GG--- 3'
3' ---GG {{pad|2em}} CGCC--- 5'

SrfI Streptomyces sp. 5' GCCCGGGC
3' CGGGCCCG 5' ---GCCC {{pad|2em}} GGGC--- 3'
3' ---CGGG {{pad|2em}} CCCG--- 5'

SrlI Selenomonas ruminantium lactilytica 84 5' GCCGGC
3' CGGCCG 5' ---G {{pad|2em}} CCGGC--- 3'
3' ---CGGCC {{pad|2em}} G--- 5'

Srl5DI Selenomonas ruminantium 5 5' CTGCAG
3' GACGTC 5' ---CTGCA {{pad|2em}} G--- 3'
3' ---G {{pad|2em}} ACGTC--- 5' AliAJI, Asp713I, BspMAI, BsuBI, CfrA4I, Pfl21I, PstI, SflI, Srl5DI

Srl32DII Selenomonas ruminantium 32 5' GAATTC
3' CTTAAG 5' ---G {{pad|2em}} AATTC--- 3'
3' ---CTTAA {{pad|2em}} G--- 5'

Srl55DI Selenomonas ruminantium lactilytica 55 5' GAATTC
3' CTTAAG 5' ---G {{pad|2em}} AATTC--- 3'
3' ---CTTAA {{pad|2em}} G--- 5'

Srl56DI Selenomonas ruminantium 56 5' CTRYAG
3' GAYRTC 5' ---C {{pad|2em}} TRYAG--- 3'
3' ---GAYRT {{pad|2em}} C--- 5'

SruI Selenomonas ruminantium 18D 5' TTTAAA
3' AAATTT 5' ---TTT {{pad|2em}} AAA--- 3'
3' ---AAA {{pad|2em}} TTT--- 5' AhaIII, DraI, PauAII

Sru4DI Selenomonas ruminantium 5' ATTAAT
3' TAATTA 5' ---AT {{pad|2em}} TAAT--- 3'
3' ---TAAT {{pad|2em}} TA--- 5' AseI, AsnI, BpoAI, PshBI, VspI

Sru30DI Selenomonas ruminantium 30 5' AGGCCT
3' TCCGGA 5' ---AGG {{pad|2em}} CCT--- 3'
3' ---TCC {{pad|2em}} GGA--- 5' AatI, AspMI, Eco147I, GdiI, PceI, Pme55I, SarI, SseBI, SteI, StuI

SsbI Streptomyces scabies 5' AAGCTT
3' TTCGAA 5' ---A {{pad|2em}} AGCTT--- 3'
3' ---TTCGA {{pad|2em}} A--- 5'

SscL1I Staphylococcus sp. L1 5' GANTC
3' CTNAG 5' ---G {{pad|2em}} ANTC--- 3'
3' ---CTNA {{pad|2em}} G--- 5'

Sse9I Sporosarcina sp. 9 5' AATT
3' TTAA 5' --- {{pad|2em}} AATT--- 3'
3' ---TTAA {{pad|2em}} --- 5'

Sse232I Streptomyces sp. RH232 5' CGCCGGCG
3' GCGGCCGC 5' ---CG {{pad|2em}} CCGGCG--- 3'
3' ---GCGGCC {{pad|2em}} GC--- 5'

Sse1825I Streptomyces sp. 5' GGGWCCC
3' CCCWGGG 5' ---GG {{pad|2em}} GWCCC--- 3'
3' ---CCCWG {{pad|2em}} GG--- 5'

Sse8387I Streptomyces sp. 8387 5' CCTGCAGG
3' GGACGTCC 5' ---CCTGCA {{pad|2em}} GG--- 3'
3' ---GG {{pad|2em}} ACGTCC--- 5'

Sse8647I Streptomyces sp. 5' AGGWCCT
3' TCCWGGA 5' ---AG {{pad|2em}} GWCCT--- 3'
3' ---TCCWG {{pad|2em}} GA--- 5'

SseAI Streptomyces sp. 5' GGCGCC
3' CCGCGG 5' ---GG {{pad|2em}} CGCC--- 3'
3' ---CCGC {{pad|2em}} GG--- 5'

SseBI Streptomyces sp. 5' AGGCCT
3' TCCGGA 5' ---AGG {{pad|2em}} CCT--- 3'
3' ---TCC {{pad|2em}} GGA--- 5' AatI, AspMI, Eco147I, GdiI, PceI, Pme55I, SarI, Sru30DI, SteI, StuI

SshAI Salmonella shikmonah 5' CCTNAGG
3' GGANTCC 5' ---CC {{pad|2em}} TNAGG--- 3'
3' ---GGANT {{pad|2em}} CC--- 5' AocI, BliHKI, BspR7I, CvnI, Lmu60I, OxaNI, SauI, SshAI

SsiI Staphylococcus sciuri RFL1 5' CCGC
3' GGCG 5' ---C {{pad|2em}} CGC--- 3'
3' ---GGC {{pad|2em}} G--- 5' AciI

SsiAI Staphylococcus sp. A 5' GATC
3' CTAG 5' --- {{pad|2em}} GATC--- 3'
3' ---CTAG {{pad|2em}} --- 5' BscFI, BspAI, BstKTI, CpfI, HacI, MkrAI, RalF40I, SauMI

SsiBI Staphylococcus sp. B 5' GATC
3' CTAG 5' --- {{pad|2em}} GATC--- 3'
3' ---CTAG {{pad|2em}} --- 5' BscFI, BspFI, BstKTI, CpfI, HacI, MkrAI, RalF40I, SauMI

SslI Streptococcus salivarius thermophilus 5' CCWGG
3' GGWCC 5' ---CC {{pad|2em}} WGG--- 3'
3' ---GGW {{pad|2em}} CC--- 5' ApaORI, BseBI, BshGI, BstNI, BstOI, Bst2UI, BthEI, EcoRII, MvaI

SsoI Shigella sonnei 47 5' GAATTC
3' CTTAAG 5' ---G {{pad|2em}} AATTC--- 3'
3' ---CTTAA {{pad|2em}} G--- 5'

SsoII Shigella sonnei 47 5' CCNGG
3' GGNCC 5' --- {{pad|2em}} CCNGG--- 3'
3' ---GGNCC {{pad|2em}} --- 5'

SspI Sphaerotilus sp. 5' AATATT
3' TTATAA 5' ---AAT {{pad|2em}} ATT--- 3'
3' ---TTA {{pad|2em}} TAA--- 5'

Ssp1I Streptomyces sp. RFL1 5' TTCGAA
3' AAGCTT 5' ---TT {{pad|2em}} CGAA--- 3'
3' ---AAGC {{pad|2em}} TT--- 5' AsuII, BimI, Bsp119I, CbiI, MlaI, PlaII, SfuI, SspRFI, SviI

Ssp4800I

Streptomyces sp. BMTU 4800

5' TGTACA
3' ACATGT

5' ---T {{pad|2em}} GTACA--- 3'
3' ---ACATG {{pad|2em}} T--- 5'

AauI,

BsmRI,

Bsp1407I,

BsrGI, BstAUI, SspBI

Ssp5230I Streptomyces sp. 5230 5' GACGTC
3' CTGCAG 5' ---GACGT {{pad|2em}} C--- 3'
3' ---C {{pad|2em}} TGCAG--- 5' AatII, ZraI

Ssp27144I Synechococcus sp. 5' ATCGAT
3' TAGCTA 5' ---AT {{pad|2em}} CGAT--- 3'
3' ---TAGC {{pad|2em}} TA--- 5' BanIII, BavCI, BbvAII, BscI, BspJI, BspOVII, BstNZ169I, ClaI, LcaI

SspAI Synechococcus sp. 5' CCWGG
3' GGWCC 5' --- {{pad|2em}} CCWGG--- 3'
3' ---GGWCC {{pad|2em}} --- 5' AeuI, AorI, Bse17I, BspNI, Bst2I, EcoRII, SniI, SslI, SspAI, ZanI

SspBI

Streptomyces sp.

5' TGTACA
3' ACATGT

5' ---T {{pad|2em}} GTACA--- 3'
3' ---ACATG {{pad|2em}} T--- 5'

AauI,

BsmRI,

Bsp1407I,

BsrGI, BstAUI, Ssp4800I

SspCI Streptomyces sp. 5' GCCGGC
3' CGGCCG 5' ---GCC {{pad|2em}} GGC--- 3'
3' ---CGG {{pad|2em}} CCG--- 5'

SspD5I Staphylococcus sp. D5 5' GGTGA
3' CCACT 5' ---GGTGAN7N {{pad|2em}} --- 3'
3' ---CCACTN7N {{pad|2em}} --- 5' AsuHPI, HphI

SspD5II Staphylococcus sp. D5 5' ATGCAT
3' TACGTA 5' ---ATGCA {{pad|2em}} T--- 3'
3' ---T {{pad|2em}} ACGTA--- 5' Csp68KIII, EcoT22I, Mph1103I, NsiI, PinBI, Ppu10I, SepI, Zsp2I

SspRFI Synechococcus sp. RF-1 5' TTCGAA
3' AAGCTT 5' ---TT {{pad|2em}} CGAA--- 3'
3' ---AAGC {{pad|2em}} TT--- 5' AsuII, BimI, Bsp119I, CbiI, MlaI, PpaAI, SfuI, Ssp1I, SviI

SsrI Staphylococcus saprophyticus B6 5' GTTAAC
3' CAATTG 5' ---GTT {{pad|2em}} AAC--- 3'
3' ---CAA {{pad|2em}} TTG--- 5'

SstI Streptomyces stanford 5' GAGCTC
3' CTCGAG 5' ---GAGCT {{pad|2em}} C--- 3'
3' ---C {{pad|2em}} TCGAG--- 5'

SstII Streptomyces stanford 5' CCGCGG
3' GGCGCC 5' ---CCGC {{pad|2em}} GG--- 3'
3' ---GG {{pad|2em}} CGCC--- 5'

Sst12I Streptomyces sp. ST-12 5' CTGCAG
3' GACGTC 5' ---CTGCA {{pad|2em}} G--- 3'
3' ---G {{pad|2em}} ACGTC--- 5' Asp713I, BloHII, BspMAI, BsuBI, CfuII, Psp23I, PstI, SflI, Srl5DI

SteI Streptomyces tendae 5' AGGCCT
3' TCCGGA 5' ---AGG {{pad|2em}} CCT--- 3'
3' ---TCC {{pad|2em}} GGA--- 5' AatI, AspMI, Eco147I, GdiI, PceI, Pme55I, SarI, Sru30DI, SseBI, StuI

SthI Salmonella thompson YY356 5' GGTACC
3' CCATGG 5' ---G {{pad|2em}} GTACC--- 3'
3' ---CCATG {{pad|2em}} G--- 5' Acc65I, AhaB8I, Asp718I, KpnI,

Sth117I Streptococcus thermophilus ST117 5' CCWGG
3' GGWCC 5' ---CC {{pad|2em}} WGG--- 3'
3' ---GGW {{pad|2em}} CC--- 5' ApaORI, BseBI, BsiLI, BstNI, BstOI, Bst2UI, EcoRII, MvaI, SspAI

Sth132I Streptococcus thermophilus 5' CCCG
3' GGGC 5' ---CCCGNNNN {{pad|2em}} NNNN--- 3'
3' ---GGGCNNNNNNNN {{pad|2em}} --- 5'

Sth134I Streptococcus thermophilus 134 5' CCGG
3' GGCC 5' ---C {{pad|2em}} CGG--- 3'
3' ---GGC {{pad|2em}} C--- 5'

Sth368I Streptococcus thermophilus CNRZ368 5' GATC
3' CTAG 5' --- {{pad|2em}} GATC--- 3'
3' ---CTAG {{pad|2em}} --- 5' BscFI, BspFI, BstKTI, CpfI, HacI, MkrAI, RalF40I, SauMI

StrI Streptomyces thermodiastaticus 5' CTCGAG
3' GAGCTC 5' ---C {{pad|2em}} TCGAG--- 3'
3' ---GAGCT {{pad|2em}} C--- 5' AbrI, BluI, BssHI, MavI, Sau3239I, Sfr274I, TliI, XhoI

StsI Streptococcus sanguis 54 5' GGATG
3' CCTAC 5' ---GGATGN8NN {{pad|2em}} NNNN--- 3'
3' ---CCTACN8NNNNNN {{pad|2em}} --- 5'

StuI^[2] Streptomyces tubercidicus 5' AGGCCT
3' TCCGGA 5' ---AGG {{pad|2em}} CCT--- 3'
3' ---TCC {{pad|2em}} GGA--- 5' AatI, AspMI, Eco147I, GdiI, PceI, SarI, Sru30DI, SseBI, SteI

StyI Salmonella typhi 5' CCWWGG
3' GGWWCC 5' ---C {{pad|2em}} CWWGG--- 3'
3' ---GGWWC {{pad|2em}} C--- 5'

StyD4I Salmonella typhi D4 5' CCNGG
3' GGNCC 5' --- {{pad|2em}} CCNGG--- 3'
3' ---GGNCC {{pad|2em}} --- 5'

SuaI Sulfolobus acidocaldarius 5' GGCC
3' CCGG 5' ---GG {{pad|2em}} CC--- 3'
3' ---CC {{pad|2em}} GG--- 5'

SuiI Sulfolobus islandicus REN2HI 5' GCWGC
3' CGWCG 5' ---G {{pad|2em}} CWGC--- 3'
3' --- CGWC {{pad|2em}} G--- 5' AceI, ApeKI, Taq52I, TseI

SunI Synechococcus uniformis 5' CGTACG
3' GCATGC 5' ---C {{pad|2em}} GTACG--- 3'
3' ---GCATG {{pad|2em}} C--- 5'

SurI Sporosarcina ureae 2 5' GGATCC
3' CCTAGG 5' ---G {{pad|2em}} GATCC--- 3'
3' ---CCTAG {{pad|2em}} G--- 5' AccEBI, AliI, ApaCI, AsiI, BamHI, Bce751I, OkrAI, RspLKII, SolI

SviI Streptomyces violochromogenes 5' TTCGAA
3' AAGCTT 5' ---TT {{pad|2em}} CGAA--- 3'
3' ---AAGC {{pad|2em}} TT--- 5' AsuII, Bpu14I, Bsp119I, CbiI, MlaI, PpaAI, SfuI, Ssp1I

SwaI Staphylococcus warneri 5' ATTTAAAT
3' TAAATTTA 5' ---ATTT {{pad|2em}} AAAT--- 3'
3' ---TAAA {{pad|2em}} TTTA--- 5'

Notes and References

Book: Monty Krieger . Matthew P Scott . Matsudaira, Paul T. . Lodish, Harvey F. . Darnell, James E. . Lawrence Zipursky . Kaiser, Chris . Arnold Berk . Molecular Cell Biology . 5th . W.H. Freeman and Company . New York . 2004 . 0-7167-4366-3 .
Shimotsu H, Takahashi H, Saito H . A new site-specific endonuclease StuI from Streptomyces tubercidicus . Gene . 11 . 3–4 . 219–25 . November 1980 . 6260571 . 10.1016/0378-1119(80)90062-1 .