Lee Byung-heon (biochemist) explained

Lee Byung-heon is a professor of biochemistry and cell biology in the school of medicine at Kyungpook National University (KNU), South Korea.^{[1] [2]} He received his M.D. license from Korean Medical Association in 1989. He received his B.S. from the school of medicine, KNU, in 1989, and his M.S. and Ph.D. in biochemistry from KNU in 1991 and 1995. He was an assistant professor in the school of medicine at Dongguk University in 1996–2001 and a visiting investigator in the Sanford-Burnham Medical Research Institute, La Jolla, United States, in 2001–2003. He joined KNU in 2003. He is currently a member of the Korean Society for Biochemistry and Molecular Biology, the American Association for Cancer Research, and the American Society of Molecular Imaging. His main research interest is “discovery of tissue-specific homing peptides using phage display and their applications to molecular imaging and targeted therapy”. He is currently carrying out projects for the identification of homing peptides to tumor and atherosclerotic plaque and of phosphatidylserine- and blood clotting factor XIIIa-specific peptide ligands. He has published over 30 peer-reviewed papers, book chapters, and review articles. He has also filed several patents.^[3]

Academic career

• March 1989 – February 1993: Teaching assistant, department of biochemistry, school of medicine, KNU, South Korea
• May 1993 – April 1996: Primary physician, Public Health Center of Changyeong-gun, Gyeongju, South Korea
• March 1996 – February 1998: Instructor, department of biochemistry, school of medicine, Dongguk University, Gyeongju, South Korea
• June 1997 – August 1997: Visiting investigator, MD Anderson Cancer Center, Houston, Texas, United States
• October 1998 – February 2002: Assistant professor, department of biochemistry, school of medicine, Dongguk University, Gyeongju, South Korea
• February 2001 – July 2003: Visiting investigator, The Burnham Institute for Medical Research, San Diego, United States
• August 2003 – present: Professor, department of biochemistry and cell biology, school of medicine, KNU, South Korea; associate dean for research and international programs, school of medicine, KNU, South Korea

Professional memberships

• 1989–present: Korean Society of Molecular Biology and Biochemistry
• 1990–present: Korean Society of Molecular Cell Biology
• 2003–present: American Association for Cancer Research
• 2005–present: American Society of Molecular Imaging

Research interests

Phage display selection of tissue-specific homing peptides and biomarker-sensing peptides:Phage peptide display is a powerful tool for identifying peptides that selectively bind to a target protein or cells (see figure below). Using this technology, our lab has identified diverse homing peptides, including the bladder tumor-targeting peptide, atherosclerotic plaque-homing peptide, and IL-4 receptor-binding peptide. We also have been working on selecting peptides that specifically recognize or sense biomarker proteins of cardiovascular diseases and cancers.

Recent publications

• 24021357 . 2013 . Acharya . B . Wang . K . Kim . IS . Kang . W . Moon . C . Lee . BH . In vivo imaging of myocardial cell death using a peptide probe and assessment of long-term heart function . Journal of Controlled Release . 10.1016/j.jconrel.2013.08.294 . 172 . 1 . 367–73.
• Jeon . J. O. . Kim . S. . Choi . E. . Shin . K. . Cha . K. . So . I. S. . Kim . S. J. . Jun . E. . Kim . D. . Ahn . H. J. . Lee . B. H. . Lee . S. H. . Kim . I. S. . Designed Nanocage Displaying Ligand-Specific Peptide Bunches for High Affinity and Biological Activity . 10.1021/nn403184u . ACS Nano . 7462–71 . 2013 . 23927443 . 7 . 9.
• Book: Lee . B. H.. Kwon . T. H. . Application of Phage Display for Ligand Peptidomics to Identify Peptide Ligands Binding to AQP2-Expressing Membrane Fractions . 10.1007/978-1-4614-7209-4_12 . The Low Molecular Weight Proteome . Methods in Molecular Biology . 1023 . 181–9 . 2013 . 978-1-4614-7167-7 . 23765627.
• Bae . S. M.. Kim . J. H. . Chung . S. W. . Byun . Y.. Kim . S. Y.. Lee . B. H.. Kim . I. S. . Park . R. W.. 10.1016/j.biomaterials.2012.11.020 . An apoptosis-homing peptide-conjugated low molecular weight heparin-taurocholate conjugate with antitumor properties . Biomaterials . 34 . 8 . 2077–2086 . 2013 . 23245333.
• 10.1016/0006-8993(90)91076-S . Mentlein . R. . Von Kolszynski . M. . Sprang . R. . Lucius . R. . Proline-specific proteases in cultivated neuronal and glial cells . Brain Research . 527 . 1 . 159–162 . 1990 . 2282478. 10367361 .
• Nam . J. -O. . Son . H. -N. . Jun . E. . Cha . K. . Lee . B. -H. . Park . R. -W. . Kim . I. -S. . 10.1158/1541-7786.MCR-11-0600 . FAS1 Domain Protein Inhibits VEGF165-Induced Angiogenesis by Targeting the Interaction between VEGFR-2 and v 3 Integrin . Molecular Cancer Research . 10 . 8 . 1010–1020 . 2012 . 22710795. free .
• Ha . Y. S.. Lee . H. Y.. An . G. I. . Kim . J. . Kwak . W. . Lee . E. J. . Lee . S. M. . Lee . B. H. . Kim . I. S. . Belay . T. . Lee . W. . Ahn . B. C. . Lee . J. . Yoo . J. . Synthesis and evaluation of a radioiodinated bladder cancer specific peptide . 10.1016/j.bmc.2012.05.049 . Bioorganic & Medicinal Chemistry . 20 . 14 . 4330–5 . 2012 . 22705190.
• Kim . J. H. . Bae . S. M. . Na . M. H. . Shin . H. . Yang . Y. J. . Min . K. H. . Choi . K. Y. . Kim . K. . Park . R. W. . Kwon . I. C. . Lee . B. H. . Hoffman . A. S. . Kim . I. S. . Facilitated intracellular delivery of peptide-guided nanoparticles in tumor tissues . 10.1016/j.jconrel.2011.09.070 . Journal of Controlled Release . 157 . 3 . 493–499 . 2012 . 21945679.
• Lee . G. Y. . Kim . J. H. . Oh . G. T. . Lee . B. H. . Kwon . I. C. . Kim . I. S. . 10.1016/j.jconrel.2011.07.010 . Molecular targeting of atherosclerotic plaques by a stabilin-2-specific peptide ligand . Journal of Controlled Release . 155 . 2 . 211–217 . 2011 . 21781994.
• Wang . K. . Na . M. H. . Hoffman . A. S. . Shim . G. . Han . S. E. . Oh . Y. K. . Kwon . I. C. . Kim . I. S. . Lee . B. H. . 10.1016/j.jconrel.2011.06.043 . In situ dose amplification by apoptosis-targeted drug delivery . Journal of Controlled Release . 154 . 3 . 214–217 . 2011 . 21763738 .
• Lee . M. J. . Wang . K. . Kim . I. S. . Lee . B. H. . Han . H. S. . Molecular Imaging of Cell Death in an Experimental Model of Parkinson's Disease with a Novel Apoptosis-Targeting Peptide . 10.1007/s11307-011-0497-z . Molecular Imaging and Biology . 14 . 2 . 147–155 . 2011 . 21567253. 2675055 .
• He . X. . Na . M. H. . Kim . J. S. . Lee . G. Y. . Park . J. Y. . Hoffman . A. S. . Nam . J. O. . Han . S. E. . Sim . G. Y. . Oh . Y. K. . Kim . I. S. . Lee . B. H. . 10.1021/mp100266g . A Novel Peptide Probe for Imaging and Targeted Delivery of Liposomal Doxorubicin to Lung Tumor . Molecular Pharmaceutics . 8 . 2 . 430–438 . 2011 . 21222482.
• Mun . G. I. . Kim . I. -S. . Lee . B. -H. . Boo . Y. C. . Endothelial Argininosuccinate Synthetase 1 Regulates Nitric Oxide Production and Monocyte Adhesion under Static and Laminar Shear Stress Conditions . 10.1074/jbc.M110.180489 . Journal of Biological Chemistry . 286 . 4 . 2536–2542 . 2010 . 21106532 . 3024748 . free .
• Wang . K. . Purushotham . S. . Lee . J. Y. . Na . M. H. . Park . H. . Oh . S. J. . Park . R. W. . Park . J. Y. . Lee . E. . Cho . B. C. . Song . M. N. . Baek . M. C. . Kwak . W. . Yoo . J. . Hoffman . A. S. . Oh . Y. K. . Kim . I. S. . Lee . B. H. . In vivo imaging of tumor apoptosis using histone H1-targeting peptide . 10.1016/j.jconrel.2010.09.010 . Journal of Controlled Release . 148 . 3 . 283–291 . 2010 . 20869411.
• Lee . E. B. . Jeon . H. S. . Yoo . S. S. . Choi . Y. Y. . Kang . H. G. . Cho . S. . Cha . S. I. . Choi . J. E. . Park . T. I. . Lee . B. H. . Park . R. W. . Kim . I. S. . Kang . Y. M. . Kim . C. H. . Jheon . S. . Jung . T. H. . Park . J. Y. . Polymorphisms in Apoptosis-Related Genes and Survival of Patients with Early-Stage Non-Small-Cell Lung Cancer . 10.1245/s10434-010-1082-4 . Annals of Surgical Oncology . 17 . 10 . 2608–2618 . 2010 . 20422457. 13354091 .
• 10.1016/0140-6736(90)91783-7 . Jesudasan . M. . John . T. J. . Gupta . B. L. . Bhujwala . R. A. . Shriniwas . Multiresistant Salmonella typhi in India . Lancet . 336 . 8709 . 252 . 1990 . 1973803. 34896690 .
• Hong . H. Y. . Lee . H. Y. . Kwak . W. . Yoo . J.. Na . M. H.. So . I. S.. Kwon . T. H.. Park . H. S.. Huh . S.. Oh . G. T.. Kwon . I. C. . Kim . I. S.. Lee . B. H.. Phage display selection of peptides that home to atherosclerotic plaques: IL-4 receptor as a candidate target in atherosclerosis . 10.1111/j.1582-4934.2008.00189.x . Journal of Cellular and Molecular Medicine . 12 . 5b . 2003–2014 . 2008 . 19012727. 4506166.
• Hong . H. Y. . Choi . J. S. . Kim . Y. J. . Lee . H. Y.. Kwak . W. . Yoo . J.. Lee . J. T. . Kwon . T. H.. Kim . I. S. . Han . H. S.. Lee . B. H. . 10.1016/j.jconrel.2008.07.020 . Detection of apoptosis in a rat model of focal cerebral ischemia using a homing peptide selected from in vivo phage display . Journal of Controlled Release . 131 . 3 . 167–172 . 2008 . 18692101.
• Thapa . N . Hong . HY . Sangeetha . P . Kim . IS . Yoo . J . Rhee . K . Oh . GT . Kwon . IC . Lee . BH. 10.1016/j.jconrel.2008.07.013 . Identification of a peptide ligand recognizing dysfunctional endothelial cells for targeting atherosclerosis . Journal of Controlled Release . 131 . 1 . 27–33 . 2008 . 18680772.
• Kwon . MK . Nam . JO . Park . RW . Lee . BH . Park . JY . Byun . YR. Kim . SY. Kwon . IC. Kim . IS. 10.1158/1535-7163.MCT-07-2009 . Antitumor effect of a transducible fusogenic peptide releasing multiple proapoptotic peptides by caspase-3 . Molecular Cancer Therapeutics . 7 . 6 . 1514–1522 . 2008 . 18566222. 15515007 .
• Lee . Y. -J. . Choi . H. -J. . Lim . J. -S. . Earm . J. -H. . Lee . B. -H. . Kim . I. -S. . Frokiaer . J. . Nielsen . S. . Kwon . T. -H. . 10.1152/ajprenal.00006.2008 . A novel method of ligand peptidomics to identify peptide ligands binding to AQP2-expressing plasma membranes and intracellular vesicles of rat kidney . AJP: Renal Physiology . 295 . 1 . F300–F309 . 2008 . 18480184 .
• Nam . J. O. . Jung . M. Y. . Thapa . N. . Lee . B. H. . Park . R. W. . Kim . I. S. . 10.3858/emm.2008.40.2.196 . T-CAM, a fastatin-FIII 9-10 fusion protein, potently enhances anti-angiogenic and anti-tumor activity αvβ3 and α5β1 integrins . Experimental & Molecular Medicine . 40 . 2 . 196–207 . 2008 . 18446058 . 2679300 .
• Thapa . N. . Kim . S. . So . I. S. . Lee . B. H. . Kwon . I. C. . Choi . K. . Kim . I. S. . 10.1111/j.1582-4934.2008.00305.x . Discovery of a phosphatidylserine-recognizing peptide and its utility in molecular imaging of tumour apoptosis . Journal of Cellular and Molecular Medicine . 12 . 5a . 1649–1660 . 2008 . 18363834 . 3918081.
• Thapa . N. . Lee . B. H. . Kim . I. S. . 10.1016/j.biocel.2007.06.004 . TGFBIp/βig-h3 protein: A versatile matrix molecule induced by TGF-β . The International Journal of Biochemistry & Cell Biology . 39 . 12 . 2183–2194 . 2007 . 17659994 .
• Park . S. -H. . Choi . M. -J. . Song . I. -K. . Choi . S. -Y. . Nam . J. -O. . Kim . C. -D. . Lee . B. -H. . Park . R. -W. . Park . K. M. . Kim . Y. -J. . Kim . I. -S. . Kwon . T. -H. . Kim . Y. -L. . Erythropoietin Decreases Renal Fibrosis in Mice with Ureteral Obstruction: Role of Inhibiting TGF-beta-Induced Epithelial-to-Mesenchymal Transition . 10.1681/ASN.2005080866 . Journal of the American Society of Nephrology . 18 . 5 . 1497–1507 . 2007 . 17389738 . free .
• Lee . S. -M. . Lee . E. -J. . Hong . H. -Y. . Kwon . M. -K. . Kwon . T. -H. . Choi . J. -Y. . Park . R. -W. . Kwon . T. -G. . Yoo . E. -S. . Yoon . G. -S. . Kim . I. -S. . Ruoslahti . E. . Lee . B. -H. . Targeting Bladder Tumor Cells in vivo and in the Urine with a Peptide Identified by Phage Display . 10.1158/1541-7786.MCR-06-0069 . Molecular Cancer Research . 5 . 1 . 11–19 . 2007 . 17259343 . free .
• Lee . B. H. . Bae . J. S. . Park . R. W. . Kim . J. E. . Park . J. Y. . Kim . I. S. . 10.1038/emm.2006.19 . Βig-h3 triggers signaling pathways mediating adhesion and migration of vascular smooth muscle cells through αvβ5 integrin . Experimental & Molecular Medicine . 38 . 2 . 153–161 . 2006 . 16672769 . free .
• Kim . Y. J. . Kim . H. N. . Park . E. K. . Lee . B. H. . Ryoo . H. M. . Kim . S. Y. . Kim . I. S. . Stein . J. L. . Lian . J. B. . Stein . G. S. . Van Wijnen . A. J. . Choi . J. Y. . 10.1016/j.gene.2005.08.021 . The bone-related Zn finger transcription factor Osterix promotes proliferation of mesenchymal cells . Gene . 366 . 1 . 145–151 . 2006 . 16314050 .
• Lee . B. H. . Ruoslahti . E. . 10.1002/jcb.20488 . Α5β1 integrin stimulates Bcl-2 expression and cell survival through Akt, focal adhesion kinase, and Ca2+/calmodulin-dependent protein kinase IV . Journal of Cellular Biochemistry . 95 . 6 . 1214–1223 . 2005 . 15962308 . 85628471 .
• Nam . J. -O. . Jeong . H. W. . Lee . B. H. . Park . R. W. . Kim . I. S. . Regulation of Tumor Angiogenesis by Fastatin, the Fourth FAS1 Domain of ig-h3, via v 3 Integrin . 10.1158/0008-5472.CAN-04-2705 . Cancer Research . 65 . 10 . 4153–4161 . 2005 . 15899806 . free .
• Lee . S. J. . Jeon . H. S. . Jang . J. S. . Park . S. H. . Lee . G. Y. . Lee . B. H. . Kim . C. H. . Kang . Y. M. . Lee . W. K. . Kam . S. . Park . R. W. . Kim . I. S. . Cho . Y. L. . Jung . T. H. . Park . J. Y. . 10.1093/carcin/bgh307 . DNMT3B polymorphisms and risk of primary lung cancer . Carcinogenesis . 26 . 2 . 403–409 . 2004 . 15528220 . free .
• Park . S. W. . Bae . J. S. . Kim . K. S. . Park . S. H. . Lee . B. H. . Choi . J. Y. . Park . J. Y. . Ha . S. W. . Kim . Y. L. . Kwon . T. H. . Kim . I. S. . Park . R. W. . 10.1038/emm.2004.29 . Beta ig-h3 promotes renal proximal tubular epithelial cell adhesion, migration and proliferation through the interaction with α3β1 integrin . Experimental & Molecular Medicine . 36 . 3 . 211–219 . 2004 . 15272232 . free .
• Lee . S. H. . Bae . J. S. . Park . S. H. . Lee . B. H. . Park . R. W. . Choi . J. Y. . Park . J. Y. . Ha . S. W. . Kim . Y. L. . Kwon . T. H. . Kim . I. S. . 10.1046/j.1523-1755.2003.00158.x . Expression of TGF-beta-induced matrix protein betaig-h3 is up-regulated in the diabetic rat kidney and human proximal tubular epithelial cells treated with high glucose . Kidney International . 64 . 3 . 1012–1021 . 2003 . 12911551 . free .
• Nam . J. -O. . Kim . J. E. . Jeong . H. W. . Lee . S. J. . Lee . B. H. . Choi . J. Y. . Park . R. W. . Park . J. Y. . Kim . I. S. . Identification of the alphavbeta3 integrin-interacting motif of betaig-h3 and its anti-angiogenic effect . 10.1074/jbc.M300358200 . Journal of Biological Chemistry . 278 . 28 . 25902–25909 . 2003 . 12704192 . free .
• Kim . J. E. . Kim . S. J. . Jeong . H. W. . Lee . B. H. . Choi . J. Y. . Park . R. W. . Park . J. Y. . Kim . I. S. . 10.1038/sj.onc.1206269 . RGD peptides released from βig-h3, a TGF-β-induced cell-adhesive molecule, mediate apoptosis . Oncogene . 22 . 13 . 2045–2053 . 2003 . 12673209 . 25129211 .
• Kim . J. -E. . Jeong . H. W. . Nam . J. O. . Lee . B. H. . Choi . J. Y. . Park . R. W. . Park . J. Y. . Kim . I. S. . 10.1074/jbc.M207055200 . Identification of Motifs in the Fasciclin Domains of the Transforming Growth Factor-beta -induced Matrix Protein beta ig-h3 That Interact with the alpha vbeta 5 Integrin . Journal of Biological Chemistry . 277 . 48 . 46159–46165 . 2002 . 12270930 . free .

Patents

• Peptides for targeting apoptotic cells and uses thereof US . 8217012. KR . 20100029232. JP . 2009273462. EP . 2279201. CN . 102177174.
• Polypeptide specifically bound to phosphatidylserine and the use thereof US . 8133971. KR . 20090091589. JP . 2011515075. EP . 2280024. CN . 101965356.
• Peptide for diagnosing, preventing and treating atherosclerosis and uses thereof US . 7968081. KR . 20080090610.
• Bladder tumor-targeting peptide and use thereof US . 7745410.
• Use of a peptide that interacts with alphaV beta3 integrin of endothelial cells EP . 1620122.
• Novel use of isolated polypeptide comprising four FAS-1 domains, EM1 domain and RGD motif US . 2008274955. WO . 2005099743.
• Target-aiming drug delivery system for diagnosis and treatment of cancer containing liposome labeled with peptides which specifically targets interleukin-4 receptors, and manufacturing method thereof US . 2012294931. EP . 520281. KR . 20110076836.
• Peptide which passes through blood-brain barrier and targets apoptosis of neurodegenerative brain disease site and uses thereof US . 2012288447. KR . 20110053119. WO . 2011059263.
• Uses of apoptotic cell-targeting peptides, label substances and liposomes containing a therapeutic agent for preventing, treating or therapeutically diagnosing apoptosis-related diseases WO . 2011059262.
• Stroke-targeting peptide and use thereof US . 2011150764. KR . 20090125440.

Current lab members

• Gowri Rangaswamy Gunassekaran: Post-doctoral fellow (from India)
• Guruprasath Padmanaban : PhD student (from India)
• Jung Hyun-Kyung (정현경): PhD student (from South Korea)
• Chi Lianhua : PhD student (from China)
• Sri Murugan Poongkavithai Vadevod: PhD student (from India)
• Md. Enamul Haque: PhD student (from Bangladesh)
• Fatima Khan: PhD student (from India)
• Smirti Gurung: PhD student (from India)
• Lee Ji-young (이지영): lab manager

Past lab members

• Sangeetha Purushotham: Post-doctoral fellow (from India)
• Bodhraj Acharya: post-doctoral fellow (from Nepal)
• Kai Wang: post-doctoral fellow (from China)
• Jung Jae-Yeop (정재엽): Master's student (from South Korea)
• Jung Joo-Hee (정주희): Master's student (from South Korea)
• Jung Yu-Min (정유민):Master's student (from South Korea)

Notes and References

1. Web site: 경북대학교 의과대학 생화학·세포생물학 교실 . Biochem.knu.ac.kr . 2012-11-01 . https://web.archive.org/web/20041009183352/http://biochem.knu.ac.kr/pro.asp . 2004-10-09 . dead .
2. Web site: 경북대학교 세포.기질연구소 . cmbl.knu.ac.kr/ . 2013-09-16 . https://web.archive.org/web/20140808124403/http://cmbl.knu.ac.kr/mem.html . 2014-08-08 . dead .
3. Web site: nanoHUB.org - members: View: Byung-Heon Lee . nanohub.org . 2013-09-15.