L-lysine cyclodeaminase explained

L-lysine cyclodeaminase (EC 4.3.1.28, rapL (gene), fkbL (gene), tubZ (gene), visC (gene)) is an enzyme with systematic name L-lysine ammonia-lyase (cyclizing; ammonia-forming).^{[1] [2] [3]} This enzyme catalyses the following chemical reaction

L-pipecolate + NH₃

This enzyme requires bound NAD⁺.

Notes and References

1. Khaw LE, Böhm GA, Metcalfe S, Staunton J, Leadlay PF . Mutational biosynthesis of novel rapamycins by a strain of Streptomyces hygroscopicus NRRL 5491 disrupted in rapL, encoding a putative lysine cyclodeaminase . Journal of Bacteriology . 180 . 4 . 809–14 . February 1998 . 9473033 . 106958 .
2. Gatto GJ, Boyne MT, Kelleher NL, Walsh CT . Biosynthesis of pipecolic acid by RapL, a lysine cyclodeaminase encoded in the rapamycin gene cluster . Journal of the American Chemical Society . 128 . 11 . 3838–47 . March 2006 . 16536560 . 10.1021/ja0587603 .
3. Tsotsou GE, Barbirato F . Biochemical characterisation of recombinant Streptomyces pristinaespiralis L-lysine cyclodeaminase . Biochimie . 89 . 5 . 591–604 . May 2007 . 17291665 . 10.1016/j.biochi.2006.12.008 .