L-lysine 6-monooxygenase (NADPH) explained
| L-lysine 6-monooxygenase (NADPH) |
| Ec Number: | 1.14.13.59 |
| Cas Number: | 64295-82-5 |
| Go Code: | 0047091 |
| Width: | 270 |
In enzymology, a L-lysine 6-monooxygenase (NADPH) is an enzyme that catalyzes the chemical reaction
L-lysine + NADPH + H+ + O2
N
6-hydroxy-L-lysine + NADP
+ + H
2O
The 4 substrates of this enzyme are L-lysine, NADPH, H+, and O2, whereas its 3 products are N6-hydroxy-L-lysine, NADP+, and H2O.
This enzyme belongs to the family of oxidoreductases, specifically those acting on paired donors, with O2 as oxidant and incorporation or reduction of oxygen. The oxygen incorporated need not be derived from O2 with NADH or NADPH as one donor, and incorporation of one atom o oxygen into the other donor. The systematic name of this enzyme class is L-lysine,NADPH:oxygen oxidoreductase (6-hydroxylating). This enzyme is also called lysine N6-hydroxylase. This enzyme participates in lysine degradation.
References
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- Macheroux P, Plattner HJ, Romaguera A, Diekmann H . 1993 . FAD and substrate analogs as probes for lysine N6-hydroxylase from Escherichia coli EN 222 . Eur. J. Biochem. . 213 . 995 - 1002 . 8504838 . 10.1111/j.1432-1033.1993.tb17846.x . 3 . free .
- 10.1016/0167-4838(93)90032-M . Thariath AM, Fatum KL, Valvano MA, Viswanatha T . 1993 . Physico-chemical characterization of a recombinant cytoplasmic form of lysine: N6-hydroxylase . Biochim. Biophys. Acta . 1203 . 27 - 35 . 8218389 . 1 .
- de Lorenzo V, Bindereif A, Paw BH, Neilands JB . 1986 . Aerobactin biosynthesis and transport genes of plasmid ColV-K30 in Escherichia coli K-12 . J. Bacteriol. . 165 . 570 - 8 . 2935523 . 2 . 214457 . 10.1128/jb.165.2.570-578.1986 .
- Marrone L, Siemann S, Beecroft M, Viswanatha T . 1996 . Specificity of lysine:N-6-hydroxylase: A hypothesis for a reactive substrate intermediate in the catalytic mechanism . Bioorganic Chemistry . 24 . 4 . 401 - 406 . 10.1006/bioo.1996.0034 .
- Goh CJ, Szczepan EW, Menhart N, Viswanatha T . 1989 . Studies on lysine:N6-hydroxylation by cell-free systems of Aerobacter aerogenes 62-1 . Biochim. Biophys. Acta . 990 . 240 - 5 . 2493814 . 3 . 10.1016/s0304-4165(89)80040-6.
