| Official Name: | Kotwali Thana |
| Native Name: | কোতোয়ালি থানা খুলনা সদর |
| Settlement Type: | Metropolitan Thana |
| Pushpin Map: | Bangladesh |
| Pushpin Map Caption: | Location in Bangladesh |
| Pushpin Label Position: | right |
| Subdivision Type: | Country |
| Subdivision Name: |  Bangladesh |
| Subdivision Type1: | Division |
| Subdivision Name1: | Khulna Division |
| Subdivision Type2: | District |
| Subdivision Name2: | Khulna District |
| Population Total: | 248220 |
| Population As Of: | 2022 |
| Population Footnotes: | [1] |
| Population Density Km2: | auto |
| Area Total Km2: | 9.45 |
| Timezone: | BST |
| Utc Offset: | +6 |
| Coordinates: | 22°N 89°W |
| Postal Code Type: | Postal code |
| Postal Code: | 9000[2] |
| Area Code: | 041[3] |
Kotwali Thana (bn|কোতোয়ালি থানা) is a thana of Khulna Metropolitan Police in the Division of Khulna, Bangladesh. It is also known as Khulna Sadar. It is the most densely populated subdistrict in the division.
Kotwali Thana is located at 22.8083°N 89.5667°W. It has 36895 households and total area 9.45 km2.[4]
At the 1991 Bangladesh census, Kotwali Thana had a population of 191,910, of whom 111,725 were aged 18 or older. Males constituted 54.87% of the population, and females 45.13%. It had an average literacy rate of 59.7% (7+ years), against the national average of 32.4%.[5] At the 2001 census it had a population of 250,651, and at the 2011 Census of Bangladesh, 224,444.[6]