| John I, Lord of Werle | |
| Noble Family: | House of Mecklenburg |
| Father: | Nicholas I of Werle |
| Mother: | Jutta of Anhalt |
| Spouse: | Sophia of Lindow-Ruppin |
John I, Lord of Werle-Parchim ( - 15 October 1283), was from 1277 to 1281 to Lord of Werle and from 1281 to 1283 and to Lord of Werle-Parchim.
He was the eldest son of Nicholas I and Jutta of Anhalt.
After his father's death in 1277, he first ruled Werle together with his brothers Henry I and Bernard I. In 1281, it was decided to divide Werle and John took over control of Werle-Parchim.
He was married with Sophia, the daughter of Count Günther of Lindow-Ruppin.
He died on 15 October 1283, and was buried in the Doberan Minster.
John's daughters are not mentioned in historic documents, only his sons