Homoaconitate hydratase explained

The enzyme homoaconitate hydratase catalyzes the chemical reaction

(1R,2S)-1-hydroxybutane-1,2,4-tricarboxylate

(Z)-but-1-ene-1,2,4-tricarboxylate + H₂O

This enzyme belongs to the family of lyases, specifically the hydro-lyases, which cleave carbon-oxygen bonds. The systematic name of this enzyme class is (1R,2S)-1-hydroxybutane-1,2,4-tricarboxylate hydro-lyase [(''Z'')-but-1-ene-1,2,4-tricarboxylate-forming]. Other names in common use include homoaconitase, cis-homoaconitase, HACN, Lys4, LysF, and 2-hydroxybutane-1,2,4-tricarboxylate hydro-lyase (incorrect). This enzyme participates in lysine biosynthesis.

References

• Strassman M, Ceci LN . 1966 . Enzymatic formation of cis-homoaconitic acid, an intermediate in lysine biosynthesis in yeast . J. Biol. Chem. . 241 . 5401 - 7 . 5954805 . 22 .
• Jia Y, Tomita T, Yamauchi K, Nishiyama M, Palmer DR . 2006 . Kinetics and product analysis of the reaction catalysed by recombinant homoaconitase from Thermus thermophilus . Biochem. J. . 396 . 479 - 85 . 16524361 . 10.1042/BJ20051711 . 3 . 1482822 .
• Zabriskie TM, Jackson MD . 2000 . Lysine biosynthesis and metabolism in fungi . Nat. Prod. Rep. . 17 . 85 - 97 . 10714900 . 10.1039/a801345d . 1 .