| Image Coa: | DEU Holtsee COA.svg |
| Coordinates: | 54.4°N 60°W |
| Image Plan: | Holtsee in RD.png |
| State: | Schleswig-Holstein |
| District: | Rendsburg-Eckernförde |
| Amt: | Hüttener Berge |
| Elevation: | 23 |
| Area: | 21.71 |
| Postal Code: | 24363 |
| Area Code: | 04351, 04357 |
| Licence: | RD |
| Gemeindeschlüssel: | 01 0 58 080 |
| Website: | www.amt-huettener- berge.de |
| Mayor: | Jens-Peter Frank |
| Party: | SPD |
Holtsee is a municipality in the district of Rendsburg-Eckernförde, in Schleswig-Holstein, Germany.
The location of Holtsee is south of the municipality of Goosefeld or Altenhof, but north of Sehestedt or Lindau, and east of Haby.