| Guadalupe Borja | |
| Office: | First Lady of Mexico |
| Term Label: | In role |
| Term Start: | December 1, 1964 |
| Term End: | November 30, 1970 |
| President: | Gustavo Díaz Ordaz |
| Predecessor: | Eva Sámano |
| Successor: | María Esther Zuno |
| Birth Name: | Guadalupe Borja Osorno |
| Birth Date: | 4 April 1915 |
| Birth Place: | Mexico City |
| Death Place: | Mexico City |
| Party: | Institutional Revolutionary Party |
| Profession: | Secretary |
Guadalupe Borja Osorno (April 4, 1915 – July 19, 1974) was First Lady of Mexico from 1964 to 1970. She was the wife of Mexican president Gustavo Díaz Ordaz.[1]