Glycoside hydrolase family 98 explained

In molecular biology, glycoside hydrolase family 98 is a family of glycoside hydrolases.

Glycoside hydrolases are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A classification system for glycoside hydrolases, based on sequence similarity, has led to the definition of >100 different families.^{[1] [2] [3]} This classification is available on the CAZy web site,^{[4] [5]} and also discussed at CAZypedia, an online encyclopedia of carbohydrate active enzymes.^{[6] [7]}

Members of glycoside hydrolase family 98 have endo-β-galactosidase activity. This family includes E-ABase from Clostridium perfringens which cleaves both blood group A and B glycotopes.^[8]

The putative catalytic domain is found to the N-terminus of a second domain, which is not expected to form part of the catalytic activity.

Notes and References

1. Henrissat B, Callebaut I, Fabrega S, Lehn P, Mornon JP, Davies G. July 1995. Conserved catalytic machinery and the prediction of a common fold for several families of glycosyl hydrolases. Proceedings of the National Academy of Sciences of the United States of America. 92. 15. 7090–4. 10.1073/pnas.92.15.7090. 41477. 7624375. 1995PNAS...92.7090H. free.
2. Davies G, Henrissat B. September 1995. Structures and mechanisms of glycosyl hydrolases. Structure. 3. 9. 853–9. 10.1016/S0969-2126(01)00220-9. 8535779. free.
3. Henrissat B, Bairoch A. June 1996. Updating the sequence-based classification of glycosyl hydrolases. The Biochemical Journal. 316. Pt 2. 695–6. 1217404. 8687420. 10.1042/bj3160695.
4. Web site: Home. CAZy.org. en. 2018-03-06.
5. Lombard V, Golaconda Ramulu H, Drula E, Coutinho PM, Henrissat B. January 2014. The carbohydrate-active enzymes database (CAZy) in 2013. Nucleic Acids Research. 42. Database issue. D490-5. 10.1093/nar/gkt1178. 24270786. 3965031.
6. Web site: Glycoside Hydrolase Family 98. CAZypedia.org. en. 2018-03-06.
7. December 2018. Ten years of CAZypedia: a living encyclopedia of carbohydrate-active enzymes. Glycobiology. 28. 1. 3–8. 10.1093/glycob/cwx089. 29040563. CAZypedia Consortium. free.
8. Anderson KM, Ashida H, Maskos K, Dell A, Li SC, Li YT . A clostridial endo-beta-galactosidase that cleaves both blood group A and B glycotopes: the first member of a new glycoside hydrolase family, GH98. . J Biol Chem . 2005 . 280 . 9 . 7720–8 . 15618227 . 10.1074/jbc.M414099200 . free .