GPR124 explained

Probable G-protein coupled receptor 124 is a protein that in humans is encoded by the GPR124 gene.^{[1] [2] [3]} It is a member of the adhesion-GPCR family of receptors. Family members are characterized by an extended extracellular region with a variable number of protein domains coupled to a TM7 domain via a domain known as the GPCR-Autoproteolysis INducing (GAIN) domain.^{[4] [5] [6]}

Interactions

GPR124 has been shown to interact with DLG1^[7] and is involved in the Wnt/β-catenin signaling pathway along with RECK.^[8] GPR124 is the predicted target of several Group IV (+)ssRNA neuroinvasive viruses; proteolytic cleavage of GPR124 by these viral proteases may be important for entry into the brain.^[9]

Zebrafish embryos with Gpr124 loss of function demonstrate severe angiogenic deficiencies in the central nervous system.

Further reading

• Nakajima D, Okazaki N, Yamakawa H, Kikuno R, Ohara O, Nagase T . Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones . DNA Research . 9 . 3 . 99–106 . June 2002 . 12168954 . 10.1093/dnares/9.3.99 . free .
• Nagase T, Kikuno R, Ishikawa K, Hirosawa M, Ohara O . Prediction of the coding sequences of unidentified human genes. XVII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro . DNA Research . 7 . 2 . 143–150 . April 2000 . 10819331 . 10.1093/dnares/7.2.143 . free .
• Yamamoto Y, Irie K, Asada M, Mino A, Mandai K, Takai Y . Direct binding of the human homologue of the Drosophila disc large tumor suppressor gene to seven-pass transmembrane proteins, tumor endothelial marker 5 (TEM5), and a novel TEM5-like protein . Oncogene . 23 . 22 . 3889–3897 . May 2004 . 15021905 . 10.1038/sj.onc.1207495 . 6082566 .
• Bjarnadóttir TK, Fredriksson R, Höglund PJ, Gloriam DE, Lagerström MC, Schiöth HB . The human and mouse repertoire of the adhesion family of G-protein-coupled receptors . Genomics . 84 . 1 . 23–33 . July 2004 . 15203201 . 10.1016/j.ygeno.2003.12.004 .
• Vallon M, Essler M . Proteolytically processed soluble tumor endothelial marker (TEM) 5 mediates endothelial cell survival during angiogenesis by linking integrin alpha(v)beta3 to glycosaminoglycans . The Journal of Biological Chemistry . 281 . 45 . 34179–34188 . November 2006 . 16982628 . 10.1074/jbc.M605291200 . free .

Notes and References

1. Carson-Walter EB, Watkins DN, Nanda A, Vogelstein B, Kinzler KW, St Croix B . Cell surface tumor endothelial markers are conserved in mice and humans . Cancer Research . 61 . 18 . 6649–6655 . September 2001 . 11559528 .
2. Fredriksson R, Gloriam DE, Höglund PJ, Lagerström MC, Schiöth HB . There exist at least 30 human G-protein-coupled receptors with long Ser/Thr-rich N-termini . Biochemical and Biophysical Research Communications . 301 . 3 . 725–734 . February 2003 . 12565841 . 10.1016/S0006-291X(03)00026-3 .
3. Web site: Entrez Gene: GPR124 G protein-coupled receptor 124.
4. Book: Stacey M, Yona S . AdhesionGPCRs: Structure to Function (Advances in Experimental Medicine and Biology) . Springer . Berlin . 2011 . 978-1-4419-7912-4 .
5. Fredriksson R, Lagerström MC, Höglund PJ, Schiöth HB . Novel human G protein-coupled receptors with long N-terminals containing GPS domains and Ser/Thr-rich regions . FEBS Letters . 531 . 3 . 407–414 . November 2002 . 12435584 . 10.1016/S0014-5793(02)03574-3 . 7449692 . free .
6. Araç D, Boucard AA, Bolliger MF, Nguyen J, Soltis SM, Südhof TC, Brunger AT . A novel evolutionarily conserved domain of cell-adhesion GPCRs mediates autoproteolysis . The EMBO Journal . 31 . 6 . 1364–1378 . March 2012 . 22333914 . 3321182 . 10.1038/emboj.2012.26 .
7. Yamamoto Y, Irie K, Asada M, Mino A, Mandai K, Takai Y . Direct binding of the human homologue of the Drosophila disc large tumor suppressor gene to seven-pass transmembrane proteins, tumor endothelial marker 5 (TEM5), and a novel TEM5-like protein . Oncogene . 23 . 22 . 3889–3897 . May 2004 . 15021905 . 10.1038/sj.onc.1207495 . 6082566 .
8. Vanhollebeke B, Stone OA, Bostaille N, Cho C, Zhou Y, Maquet E, Gauquier A, Cabochette P, Fukuhara S, Mochizuki N, Nathans J, Stainier DY . Tip cell-specific requirement for an atypical Gpr124- and Reck-dependent Wnt/β-catenin pathway during brain angiogenesis . eLife . 4 . e06489 . June 2015 . 26051822 . 4456509 . 10.7554/eLife.06489 . Rossant J . free .
9. Doctor KZ, Gilmour E, Recarte M, Beatty TR, Shifa I, Stangel M, Schwisow J, Leary DH, Legler PM . Automated SSHHPS Analysis Predicts a Potential Host Protein Target Common to Several Neuroinvasive (+)ssRNA Viruses . Viruses . 15 . 2 . 542 . February 2023 . 36851756 . 9961674 . 10.3390/v15020542 . free .