FHOD1 explained

FH1/FH2 domain-containing protein 1 is a protein that in humans is encoded by the FHOD1 gene.^{[1] [2]}

This gene encodes a protein which is a member of the formin/diaphanous family of proteins. The gene is ubiquitously expressed but is found in abundance in the spleen. The encoded protein has sequence homology to diaphanous and formin proteins within the Formin Homology (FH)1 and FH2 domains. It also contains a coiled-coil domain, a collagen-like domain, two nuclear localization signals, and several potential PKC and PKA phosphorylation sites. It is a predominantly cytoplasmic protein and is expressed in a variety of human cell lines.^[3]

Interactions

FHOD1 has been shown to interact with RAC1.^[4]

Further reading

• Westendorf JJ . The formin/diaphanous-related protein, FHOS, interacts with Rac1 and activates transcription from the serum response element . The Journal of Biological Chemistry . 276 . 49 . 46453–9 . December 2001 . 11590143 . 10.1074/jbc.M105162200 . free .
• Koka S, Neudauer CL, Li X, Lewis RE, McCarthy JB, Westendorf JJ . The formin-homology-domain-containing protein FHOD1 enhances cell migration . Journal of Cell Science . 116 . Pt 9 . 1745–55 . May 2003 . 12665555 . 10.1242/jcs.00386 . free .
• Gevaert K, Goethals M, Martens L, Van Damme J, Staes A, Thomas GR, Vandekerckhove J . Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides . Nature Biotechnology . 21 . 5 . 566–9 . May 2003 . 12665801 . 10.1038/nbt810 . 23783563 .
• Tojo H, Kaieda I, Hattori H, Katayama N, Yoshimura K, Kakimoto S, Fujisawa Y, Presman E, Brooks CC, Pilch PF . 6 . The Formin family protein, formin homolog overexpressed in spleen, interacts with the insulin-responsive aminopeptidase and profilin IIa . Molecular Endocrinology . 17 . 7 . 1216–29 . July 2003 . 12677009 . 10.1210/me.2003-0056 .
• Takeya R, Sumimoto H . Fhos, a mammalian formin, directly binds to F-actin via a region N-terminal to the FH1 domain and forms a homotypic complex via the FH2 domain to promote actin fiber formation . Journal of Cell Science . 116 . Pt 22 . 4567–75 . November 2003 . 14576350 . 10.1242/jcs.00769 . 25675089 .
• Lehner B, Semple JI, Brown SE, Counsell D, Campbell RD, Sanderson CM . Analysis of a high-throughput yeast two-hybrid system and its use to predict the function of intracellular proteins encoded within the human MHC class III region . Genomics . 83 . 1 . 153–67 . January 2004 . 14667819 . 10.1016/S0888-7543(03)00235-0 .
• Katoh M, Katoh M . Identification and characterization of human FHOD3 gene in silico . International Journal of Molecular Medicine . 13 . 4 . 615–20 . April 2004 . 15010865 . 10.3892/ijmm.13.4.615 .
• Wang Y, El-Zaru MR, Surks HK, Mendelsohn ME . Formin homology domain protein (FHOD1) is a cyclic GMP-dependent protein kinase I-binding protein and substrate in vascular smooth muscle cells . The Journal of Biological Chemistry . 279 . 23 . 24420–6 . June 2004 . 15051728 . 10.1074/jbc.M313823200 . free .
• Westendorf JJ, Koka S . Identification of FHOD1-binding proteins and mechanisms of FHOD1-regulated actin dynamics . Journal of Cellular Biochemistry . 92 . 1 . 29–41 . May 2004 . 15095401 . 10.1002/jcb.20031 . 40202935 .
• Gill MB, Roecklein-Canfield J, Sage DR, Zambela-Soediono M, Longtine N, Uknis M, Fingeroth JD . EBV attachment stimulates FHOS/FHOD1 redistribution and co-aggregation with CD21: formin interactions with the cytoplasmic domain of human CD21 . Journal of Cell Science . 117 . Pt 13 . 2709–20 . June 2004 . 15138285 . 10.1242/jcs.01113 . free .
• Brandenberger R, Wei H, Zhang S, Lei S, Murage J, Fisk GJ, Li Y, Xu C, Fang R, Guegler K, Rao MS, Mandalam R, Lebkowski J, Stanton LW . 6 . Transcriptome characterization elucidates signaling networks that control human ES cell growth and differentiation . Nature Biotechnology . 22 . 6 . 707–16 . June 2004 . 15146197 . 10.1038/nbt971 . 27764390 .
• Lehner B, Sanderson CM . A protein interaction framework for human mRNA degradation . Genome Research . 14 . 7 . 1315–23 . July 2004 . 15231747 . 442147 . 10.1101/gr.2122004 .
• Beausoleil SA, Jedrychowski M, Schwartz D, Elias JE, Villén J, Li J, Cohn MA, Cantley LC, Gygi SP . 6 . Large-scale characterization of HeLa cell nuclear phosphoproteins . Proceedings of the National Academy of Sciences of the United States of America . 101 . 33 . 12130–5 . August 2004 . 15302935 . 514446 . 10.1073/pnas.0404720101 . 2004PNAS..10112130B . free .
• Madrid R, Gasteier JE, Bouchet J, Schröder S, Geyer M, Benichou S, Fackler OT . Oligomerization of the diaphanous-related formin FHOD1 requires a coiled-coil motif critical for its cytoskeletal and transcriptional activities . FEBS Letters . 579 . 2 . 441–8 . January 2005 . 15642356 . 10.1016/j.febslet.2004.12.009 . 23740212 .
• Gasteier JE, Schroeder S, Muranyi W, Madrid R, Benichou S, Fackler OT . FHOD1 coordinates actin filament and microtubule alignment to mediate cell elongation . Experimental Cell Research . 306 . 1 . 192–202 . May 2005 . 15878344 . 10.1016/j.yexcr.2005.02.006 .
• Schönichen A, Alexander M, Gasteier JE, Cuesta FE, Fackler OT, Geyer M . Biochemical characterization of the diaphanous autoregulatory interaction in the formin homology protein FHOD1 . The Journal of Biological Chemistry . 281 . 8 . 5084–93 . February 2006 . 16361249 . 10.1074/jbc.M509226200 . free .

Notes and References

1. Westendorf JJ, Mernaugh R, Hiebert SW . Identification and characterization of a protein containing formin homology (FH1/FH2) domains . Gene . 232 . 2 . 173–82 . May 1999 . 10352228 . 10.1016/S0378-1119(99)00127-4 .
2. Boehm MB, Milius TJ, Zhou Y, Westendorf JJ, Koka S . The mammalian formin FHOD1 interacts with the ERK MAP kinase pathway . Biochemical and Biophysical Research Communications . 335 . 4 . 1090–4 . October 2005 . 16112087 . 10.1016/j.bbrc.2005.07.191 .
3. Web site: Entrez Gene: FHOD1 formin homology 2 domain containing 1.
4. Westendorf JJ . The formin/diaphanous-related protein, FHOS, interacts with Rac1 and activates transcription from the serum response element . The Journal of Biological Chemistry . 276 . 49 . 46453–9 . December 2001 . 11590143 . 10.1074/jbc.M105162200 . free .