EEF1G explained

Elongation factor 1-gamma is a protein that in humans is encoded by the EEF1G gene.^{[1] [2] [3]}

Function

This gene encodes a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit contains an N-terminal glutathione transferase domain, which may be involved in regulating the assembly of multisubunit complexes containing this elongation factor and aminoacyl-tRNA synthetases.

Interactions

EEF1G has been shown to interact with:

• EEF1B2,^[4]
• EEF1D,^[4]
• HARS,^[5]
• LZTS1,^[6]
• LARS,^[5] and
• RECQL5.^[7]

References

Further reading

• Lew Y, Jones DV, Mars WM, Evans D, Byrd D, Frazier ML . Expression of elongation factor-1 gamma-related sequence in human pancreatic cancer. . Pancreas . 7 . 2 . 144–52 . 1992 . 1372736 . 10.1097/00006676-199203000-00003 . 44548750 .
• Koonin EV, Mushegian AR, Tatusov RL, Altschul SF, Bryant SH, Bork P, Valencia A . Eukaryotic translation elongation factor 1 gamma contains a glutathione transferase domain--study of a diverse, ancient protein superfamily using motif search and structural modeling. . Protein Sci. . 3 . 11 . 2045–54 . 1995 . 7703850 . 2142650 . 10.1002/pro.5560031117 .
• Sheu GT, Traugh JA . Recombinant subunits of mammalian elongation factor 1 expressed in Escherichia coli. Subunit interactions, elongation activity, and phosphorylation by protein kinase CKII. . J. Biol. Chem. . 272 . 52 . 33290–7 . 1998 . 9407120 . 10.1074/jbc.272.52.33290 . free .
• Sheu GT, Traugh JA . A structural model for elongation factor 1 (EF-1) and phosphorylation by protein kinase CKII. . Mol. Cell. Biochem. . 191 . 1–2 . 181–6 . 1999 . 10094407 . 10.1023/A:1006802125856 . 775166 .
• Kim JE, Kim KH, Lee SW, Seol W, Shiba K, Kim S . An elongation factor-associating domain is inserted into human cysteinyl-tRNA synthetase by alternative splicing. . Nucleic Acids Res. . 28 . 15 . 2866–72 . 2000 . 10908348 . 102683 . 10.1093/nar/28.15.2866 .
• Ishii H, Vecchione A, Murakumo Y, Baldassarre G, Numata S, Trapasso F, Alder H, Baffa R, Croce CM . FEZ1/LZTS1 gene at 8p22 suppresses cancer cell growth and regulates mitosis. . Proc. Natl. Acad. Sci. U.S.A. . 98 . 18 . 10374–9 . 2001 . 11504921 . 56968 . 10.1073/pnas.181222898 . free . 2001PNAS...9810374I .
• Sang Lee J, Gyu Park S, Park H, Seol W, Lee S, Kim S . Interaction network of human aminoacyl-tRNA synthetases and subunits of elongation factor 1 complex. . Biochem. Biophys. Res. Commun. . 291 . 1 . 158–64 . 2002 . 11829477 . 10.1006/bbrc.2002.6398 .
• Gevaert K, Goethals M, Martens L, Van Damme J, Staes A, Thomas GR, Vandekerckhove J . Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides. . Nat. Biotechnol. . 21 . 5 . 566–9 . 2004 . 12665801 . 10.1038/nbt810 . 23783563 .
• Vanwetswinkel S, Kriek J, Andersen GR, Dijk J, Siegal G . 1H, 15N and 13C resonance assignments of the highly conserved 19 kDa C-terminal domain from human elongation factor 1Bgamma. . J. Biomol. NMR . 26 . 2 . 189–90 . 2004 . 12766415 . 10.1023/A:1023504611632 . 82866448 .
• Zhang C, Dowd DR, Staal A, Gu C, Lian JB, van Wijnen AJ, Stein GS, MacDonald PN . Nuclear coactivator-62 kDa/Ski-interacting protein is a nuclear matrix-associated coactivator that may couple vitamin D receptor-mediated transcription and RNA splicing. . J. Biol. Chem. . 278 . 37 . 35325–36 . 2003 . 12840015 . 10.1074/jbc.M305191200 . free .
• Colland F, Jacq X, Trouplin V, Mougin C, Groizeleau C, Hamburger A, Meil A, Wojcik J, Legrain P, Gauthier JM . Functional proteomics mapping of a human signaling pathway. . Genome Res. . 14 . 7 . 1324–32 . 2004 . 15231748 . 442148 . 10.1101/gr.2334104 .
• Jin J, Smith FD, Stark C, Wells CD, Fawcett JP, Kulkarni S, Metalnikov P, O'Donnell P, Taylor P, Taylor L, Zougman A, Woodgett JR, Langeberg LK, Scott JD, Pawson T . Proteomic, functional, and domain-based analysis of in vivo 14-3-3 binding proteins involved in cytoskeletal regulation and cellular organization. . Curr. Biol. . 14 . 16 . 1436–50 . 2004 . 15324660 . 10.1016/j.cub.2004.07.051 . 2371325 . free . 2004CBio...14.1436J .
• Bernhard Landwehrmeyer . Goehler H, Lalowski M, Stelzl U, Waelter S, Stroedicke M, Worm U, Droege A, Lindenberg KS, Knoblich M, Haenig C, Herbst M, Suopanki J, Scherzinger E, Abraham C, Bauer B, Hasenbank R, Fritzsche A, Ludewig AH, Büssow K, Buessow K, Coleman SH, Gutekunst CA, Landwehrmeyer BG, Lehrach H, Wanker EE . A protein interaction network links GIT1, an enhancer of huntingtin aggregation, to Huntington's disease. . Mol. Cell . 15 . 6 . 853–65 . 2004 . 15383276 . 10.1016/j.molcel.2004.09.016 . free .
• Andersen JS, Lam YW, Leung AK, Ong SE, Lyon CE, Lamond AI, Mann M . Nucleolar proteome dynamics. . Nature . 433 . 7021 . 77–83 . 2005 . 15635413 . 10.1038/nature03207 . 2005Natur.433...77A . 4344740 .

Notes and References

1. Kumabe T, Sohma Y, Yamamoto T . Human cDNAs encoding elongation factor 1 gamma and the ribosomal protein L19 . Nucleic Acids Res . 20 . 10 . 2598 . July 1992 . 1598220 . 312399 . 10.1093/nar/20.10.2598 .
2. Sanders J, Maassen JA, Möller W . Elongation factor-1 messenger-RNA levels in cultured cells are high compared to tissue and are not drastically affected further by oncogenic transformation . Nucleic Acids Res . 20 . 22 . 5907–10 . January 1993 . 1461723 . 334453 . 10.1093/nar/20.22.5907 .
3. Web site: Entrez Gene: EEF1G eukaryotic translation elongation factor 1 gamma.
4. Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, Berriz GF, Gibbons FD, Dreze M, Ayivi-Guedehoussou N, Klitgord N, Simon C, Boxem M, Milstein S, Rosenberg J, Goldberg DS, Zhang LV, Wong SL, Franklin G, Li S, Albala JS, Lim J, Fraughton C, Llamosas E, Cevik S, Bex C, Lamesch P, Sikorski RS, Vandenhaute J, Zoghbi HY, Smolyar A, Bosak S, Sequerra R, Doucette-Stamm L, Cusick ME, Hill DE, Roth FP, Vidal M . Towards a proteome-scale map of the human protein-protein interaction network . Nature . 437 . 7062 . 1173–8 . October 2005 . 16189514 . 10.1038/nature04209 . 2005Natur.437.1173R . 4427026 .
5. Sang Lee J, Gyu Park S, Park H, Seol W, Lee S, Kim S . Interaction network of human aminoacyl-tRNA synthetases and subunits of elongation factor 1 complex . Biochem. Biophys. Res. Commun. . 291 . 1 . 158–64 . February 2002 . 11829477 . 10.1006/bbrc.2002.6398 .
6. Ishii H, Vecchione A, Murakumo Y, Baldassarre G, Numata S, Trapasso F, Alder H, Baffa R, Croce CM . FEZ1/LZTS1 gene at 8p22 suppresses cancer cell growth and regulates mitosis . Proc. Natl. Acad. Sci. U.S.A. . 98 . 18 . 10374–9 . August 2001 . 11504921 . 56968 . 10.1073/pnas.181222898 . free . 2001PNAS...9810374I .
7. Stelzl U, Worm U, Lalowski M, Haenig C, Brembeck FH, Goehler H, Stroedicke M, Zenkner M, Schoenherr A, Koeppen S, Timm J, Mintzlaff S, Abraham C, Bock N, Kietzmann S, Goedde A, Toksöz E, Droege A, Krobitsch S, Korn B, Birchmeier W, Lehrach H, Wanker EE . A human protein-protein interaction network: a resource for annotating the proteome . Cell . 122 . 6 . 957–68 . September 2005 . 16169070 . 10.1016/j.cell.2005.08.029 . 11858/00-001M-0000-0010-8592-0 . 8235923 . free .