DMAP1 explained

DNA methyltransferase 1-associated protein 1 is an enzyme that in humans is encoded by the DMAP1 gene.^{[1] [2] [3]}

Function

This gene encodes a subunit of several, distinct complexes involved in the repression or activation of transcription. The encoded protein can independently repress transcription and is targeted to replication foci throughout S phase by interacting directly with the N-terminus of DNA methyltransferase 1. During late S phase, histone deacetylase 2 is added to this complex, providing a means to deacetylate histones in transcriptionally inactive heterochromatin following replication. The encoded protein is also a component of the nucleosome acetyltransferase of H4 complex and interacts with the transcriptional corepressor tumor susceptibility gene 101 and the pro-apoptotic death-associated protein 6, among others. Alternatively spliced transcript variants encoding the same protein have been described.

Interactions

DMAP1 has been shown to interact with:

• C19orf2,^[4]
• DNMT1,^[5]
• ING1,^[6] and
• RGS6^[7]

Further reading

• Doyon Y, Côté J . The highly conserved and multifunctional NuA4 HAT complex. . Curr. Opin. Genet. Dev. . 14 . 2 . 147–54 . 2004 . 15196461 . 10.1016/j.gde.2004.02.009 .
• Hartley JL, Temple GF, Brasch MA . DNA cloning using in vitro site-specific recombination. . Genome Res. . 10 . 11 . 1788–95 . 2001 . 11076863 . 10.1101/gr.143000 . 310948 .
• Wiemann S, Weil B, Wellenreuther R . Toward a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs. . Genome Res. . 11 . 3 . 422–35 . 2001 . 11230166 . 10.1101/gr.GR1547R . 311072 . etal.
• Yang CS, Weiner H . Yeast two-hybrid screening identifies binding partners of human Tom34 that have ATPase activity and form a complex with Tom34 in the cytosol. . Arch. Biochem. Biophys. . 400 . 1 . 105–10 . 2002 . 11913976 . 10.1006/abbi.2002.2778 .
• Strausberg RL, Feingold EA, Grouse LH . Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. . Proc. Natl. Acad. Sci. U.S.A. . 99 . 26 . 16899–903 . 2003 . 12477932 . 10.1073/pnas.242603899 . 139241 . 2002PNAS...9916899M . etal. free .
• Cai Y, Jin J, Tomomori-Sato C . Identification of new subunits of the multiprotein mammalian TRRAP/TIP60-containing histone acetyltransferase complex. . J. Biol. Chem. . 278 . 44 . 42733–6 . 2003 . 12963728 . 10.1074/jbc.C300389200 . etal. free .
• Xin H, Yoon HG, Singh PB . Components of a pathway maintaining histone modification and heterochromatin protein 1 binding at the pericentric heterochromatin in Mammalian cells. . J. Biol. Chem. . 279 . 10 . 9539–46 . 2004 . 14665632 . 10.1074/jbc.M311587200 . etal. free .
• Ota T, Suzuki Y, Nishikawa T . Complete sequencing and characterization of 21,243 full-length human cDNAs. . Nat. Genet. . 36 . 1 . 40–5 . 2004 . 14702039 . 10.1038/ng1285 . etal. free .
• Liu Z, Fisher RA . RGS6 interacts with DMAP1 and DNMT1 and inhibits DMAP1 transcriptional repressor activity. . J. Biol. Chem. . 279 . 14 . 14120–8 . 2004 . 14734556 . 10.1074/jbc.M309547200 . free .
• Doyon Y, Selleck W, Lane WS . Structural and functional conservation of the NuA4 histone acetyltransferase complex from yeast to humans. . Mol. Cell. Biol. . 24 . 5 . 1884–96 . 2004 . 14966270 . 10.1128/MCB.24.5.1884-1896.2004 . 350560 . etal.
• Muromoto R, Sugiyama K, Takachi A . Physical and functional interactions between Daxx and DNA methyltransferase 1-associated protein, DMAP1. . J. Immunol. . 172 . 5 . 2985–93 . 2004 . 14978102 . 10.4049/jimmunol.172.5.2985. etal. free .
• Muromoto R, Sugiyama K, Yamamoto T . Physical and functional interactions between Daxx and TSG101. . Biochem. Biophys. Res. Commun. . 316 . 3 . 827–33 . 2004 . 15033475 . 10.1016/j.bbrc.2004.02.126 . etal. 2115/28120 . free .
• Ballif BA, Villén J, Beausoleil SA . Phosphoproteomic analysis of the developing mouse brain. . Mol. Cell. Proteomics . 3 . 11 . 1093–101 . 2005 . 15345747 . 10.1074/mcp.M400085-MCP200 . etal. free .
• Delgermaa L, Hayashi N, Dorjsuren D . Subcellular localization of RPB5-mediating protein and its putative functional partner. . Mol. Cell. Biol. . 24 . 19 . 8556–66 . 2004 . 15367675 . 10.1128/MCB.24.19.8556-8566.2004 . 516735 . etal.
• Gerhard DS, Wagner L, Feingold EA . The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). . Genome Res. . 14 . 10B . 2121–7 . 2004 . 15489334 . 10.1101/gr.2596504 . 528928 . etal.
• Wiemann S, Arlt D, Huber W . From ORFeome to biology: a functional genomics pipeline. . Genome Res. . 14 . 10B . 2136–44 . 2004 . 15489336 . 10.1101/gr.2576704 . 528930 . etal.
• Cai Y, Jin J, Florens L . The mammalian YL1 protein is a shared subunit of the TRRAP/TIP60 histone acetyltransferase and SRCAP complexes. . J. Biol. Chem. . 280 . 14 . 13665–70 . 2005 . 15647280 . 10.1074/jbc.M500001200 . etal. free .

Notes and References

1. Rountree MR, Bachman KE, Baylin SB . DNMT1 binds HDAC2 and a new co-repressor, DMAP1, to form a complex at replication foci . Nat Genet . 25 . 3 . 269–77 . Aug 2000 . 10888872 . 10.1038/77023 . 26149386 .
2. Nagase T, Kikuno R, Ishikawa KI, Hirosawa M, Ohara O . Prediction of the coding sequences of unidentified human genes. XVI. The complete sequences of 150 new cDNA clones from brain which code for large proteins in vitro . DNA Res . 7 . 1 . 65–73 . Apr 2000 . 10718198 . 10.1093/dnares/7.1.65 . free .
3. Web site: Entrez Gene: DMAP1 DNA methyltransferase 1 associated protein 1.
4. Oct 2004 . Delgermaa L, Hayashi N, Dorjsuren D, Nomura T, Thuy le TT, Murakami S . Subcellular localization of RPB5-mediating protein and its putative functional partner . Mol. Cell. Biol. . 24 . 19 . 8556–66 . 15367675 . 516735 . 10.1128/MCB.24.19.8556-8566.2004.
5. Jul 2000 . Rountree MR, Bachman KE, Baylin SB . DNMT1 binds HDAC2 and a new co-repressor, DMAP1, to form a complex at replication foci . Nat. Genet. . 25 . 3 . 269–77 . 10888872 . 10.1038/77023. 26149386 .
6. Mar 2004 . Xin H, Yoon HG, Singh PB, Wong J, Qin J . Components of a pathway maintaining histone modification and heterochromatin protein 1 binding at the pericentric heterochromatin in Mammalian cells . J. Biol. Chem. . 279 . 10 . 9539–46 . 14665632 . 10.1074/jbc.M311587200. free .
7. Apr 2004 . Liu Z, Fisher RA . RGS6 interacts with DMAP1 and DNMT1 and inhibits DMAP1 transcriptional repressor activity . J. Biol. Chem. . 279 . 14 . 14120–8 . 14734556 . 10.1074/jbc.M309547200. free .