DLGAP5 explained

Disks large-associated protein 5 (DAP-5) also known as discs large homolog 7 (DLG7) or hepatoma up-regulated protein (HURP) is a protein that in humans is encoded by the DLGAP5 gene.^{[1] [2] [3] [4]}

DLG7 is a kinetochore protein that stabilizes microtubules in vicinity of chromosomes. DLG7 controls spindle dynamics, promotes interkinetochore tension and efficient kinetochore capture.^[5] DGL7 is a part of Ran-dependent complex.^[6] Stabilization of DGL7 in cell occurs due to phosphorylation by Aurora A kinase.^[7] Expression of DGL7 is found in cancer^[8] and stem cells.^[9]

Further reading

• Ohara O, Nagase T, Ishikawa K . Construction and characterization of human brain cDNA libraries suitable for analysis of cDNA clones encoding relatively large proteins . DNA Res. . 4 . 1 . 53–9 . 1997 . 9179496 . 10.1093/dnares/4.1.53 . etal. free .
• Bassal S, Nomura N, Venter D . Characterization of a novel human cell-cycle-regulated homologue of Drosophila dlg1 . Genomics . 77 . 1–2 . 5–7 . 2001 . 11543626 . 10.1006/geno.2001.6570 . etal.
• Strausberg RL, Feingold EA, Grouse LH . Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences . Proc. Natl. Acad. Sci. U.S.A. . 99 . 26 . 16899–903 . 2003 . 12477932 . 10.1073/pnas.242603899 . 139241 . 2002PNAS...9916899M . etal. free .
• Tsou AP, Yang CW, Huang CY . Identification of a novel cell cycle regulated gene, HURP, overexpressed in human hepatocellular carcinoma . Oncogene . 22 . 2 . 298–307 . 2003 . 12527899 . 10.1038/sj.onc.1206129 . 24615395 . etal.
• Mantovani F, Banks L . Regulation of the discs large tumor suppressor by a phosphorylation-dependent interaction with the beta-TrCP ubiquitin ligase receptor . J. Biol. Chem. . 278 . 43 . 42477–86 . 2004 . 12902344 . 10.1074/jbc.M302799200 . free .
• Laprise P, Viel A, Rivard N . Human homolog of disc-large is required for adherens junction assembly and differentiation of human intestinal epithelial cells . J. Biol. Chem. . 279 . 11 . 10157–66 . 2004 . 14699157 . 10.1074/jbc.M309843200 . free .
• Shu H, Chen S, Bi Q . Identification of phosphoproteins and their phosphorylation sites in the WEHI-231 B lymphoma cell line . Mol. Cell. Proteomics . 3 . 3 . 279–86 . 2004 . 14729942 . 10.1074/mcp.D300003-MCP200 . etal. free .
• Hsu JM, Lee YC, Yu CT, Huang CY . Fbx7 functions in the SCF complex regulating Cdk1-cyclin B-phosphorylated hepatoma up-regulated protein (HURP) proteolysis by a proline-rich region . J. Biol. Chem. . 279 . 31 . 32592–602 . 2004 . 15145941 . 10.1074/jbc.M404950200 . free .
• Blot V, Delamarre L, Perugi F . Human Dlg protein binds to the envelope glycoproteins of human T-cell leukemia virus type 1 and regulates envelope mediated cell-cell fusion in T lymphocytes . J. Cell Sci. . 117 . Pt 17 . 3983–93 . 2005 . 15286176 . 10.1242/jcs.01266 . 23757741 . etal.
• Beausoleil SA, Jedrychowski M, Schwartz D . Large-scale characterization of HeLa cell nuclear phosphoproteins . Proc. Natl. Acad. Sci. U.S.A. . 101 . 33 . 12130–5 . 2004 . 15302935 . 10.1073/pnas.0404720101 . 514446 . 2004PNAS..10112130B . etal. free .
• Zhao L, Qin LX, Ye QH . KIAA0008 gene is associated with invasive phenotype of human hepatocellular carcinoma--a functional analysis . J. Cancer Res. Clin. Oncol. . 130 . 12 . 719–27 . 2005 . 15340842 . 10.1007/s00432-004-0595-2 . 24151704 . etal.
• Gerhard DS, Wagner L, Feingold EA . The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC) . Genome Res. . 14 . 10B . 2121–7 . 2004 . 15489334 . 10.1101/gr.2596504 . 528928 . etal.
• Yu CT, Hsu JM, Lee YC . Phosphorylation and stabilization of HURP by Aurora-A: implication of HURP as a transforming target of Aurora-A . Mol. Cell. Biol. . 25 . 14 . 5789–800 . 2005 . 15987997 . 10.1128/MCB.25.14.5789-5800.2005 . 1168800 . etal.
• Rual JF, Venkatesan K, Hao T . Towards a proteome-scale map of the human protein-protein interaction network . Nature . 437 . 7062 . 1173–8 . 2005 . 16189514 . 10.1038/nature04209 . 2005Natur.437.1173R . 4427026 . etal.
• Nousiainen M, Silljé HH, Sauer G . Phosphoproteome analysis of the human mitotic spindle . Proc. Natl. Acad. Sci. U.S.A. . 103 . 14 . 5391–6 . 2006 . 16565220 . 10.1073/pnas.0507066103 . 1459365 . 2006PNAS..103.5391N . etal. free .
• Wong J, Fang G . HURP controls spindle dynamics to promote proper interkinetochore tension and efficient kinetochore capture . J. Cell Biol. . 173 . 6 . 879–91 . 2006 . 16769820 . 10.1083/jcb.200511132 . 2063914 .
• Beausoleil SA, Villén J, Gerber SA . A probability-based approach for high-throughput protein phosphorylation analysis and site localization . Nat. Biotechnol. . 24 . 10 . 1285–92 . 2006 . 16964243 . 10.1038/nbt1240 . 14294292 . etal.
• Olsen JV, Blagoev B, Gnad F . Global, in vivo, and site-specific phosphorylation dynamics in signaling networks . Cell . 127 . 3 . 635–48 . 2006 . 17081983 . 10.1016/j.cell.2006.09.026 . etal. free .

Notes and References

1. Nomura N, Miyajima N, Sazuka T, Tanaka A, Kawarabayasi Y, Sato S, Nagase T, Seki N, Ishikawa K, Tabata S . Prediction of the coding sequences of unidentified human genes. I. The coding sequences of 40 new genes (KIAA0001-KIAA0040) deduced by analysis of randomly sampled cDNA clones from human immature myeloid cell line KG-1 . DNA Res . 1 . 1 . 27–35 . Dec 1995 . 7584026 . 10.1093/dnares/1.1.27 . free .
2. Nomura N, Miyajima N, Sazuka T, Tanaka A, Kawarabayasi Y, Sato S, Nagase T, Seki N, Ishikawa K, Tabata S . Prediction of the coding sequences of unidentified human genes. I. The coding sequences of 40 new genes (KIAA0001-KIAA0040) deduced by analysis of randomly sampled cDNA clones from human immature myeloid cell line KG-1 (supplement) . DNA Res . 1 . 1 . 47–56 . Dec 1995 . 7584028 . 10.1093/dnares/1.1.47 . free .
3. Web site: Entrez Gene: DLG7 discs, large homolog 7 (Drosophila).
4. Bassal S, Nomura N, Venter D, Brand K, McKay MJ, van der Spek PJ . Characterization of a novel human cell-cycle-regulated homologue of Drosophila dlg1 . Genomics . 77 . 1–2 . 5–7 . September 2001 . 11543626 . 10.1006/geno.2001.6570 .
5. Wilde A . "HURP on" we're off to the kinetochore! . J. Cell Biol. . 173 . 6 . 829–31 . June 2006 . 16785318 . 2063907 . 10.1083/jcb.200605150 .
6. Koffa MD, Casanova CM, Santarella R, Köcher T, Wilm M, Mattaj IW . HURP is part of a Ran-dependent complex involved in spindle formation . Curr. Biol. . 16 . 8 . 743–54 . April 2006 . 16631581 . 10.1016/j.cub.2006.03.056 . free . 2006CBio...16..743K .
7. Yu CT, Hsu JM, Lee YC, Tsou AP, Chou CK, Huang CY . Phosphorylation and stabilization of HURP by Aurora-A: implication of HURP as a transforming target of Aurora-A . Mol. Cell. Biol. . 25 . 14 . 5789–800 . July 2005 . 15987997 . 1168800 . 10.1128/MCB.25.14.5789-5800.2005 .
8. Sanderson HS, Clarke PR . Cell biology: Ran, mitosis and the cancer connection . Curr. Biol. . 16 . 12 . R466–8 . June 2006 . 16782004 . 10.1016/j.cub.2006.05.032 . free . 2006CBio...16.R466S .
9. Gudmundsson KO, Thorsteinsson L, Sigurjonsson OE . Gene expression analysis of hematopoietic progenitor cells identifies Dlg7 as a potential stem cell gene . Stem Cells . 25 . 6 . 1498–506 . June 2007 . 17322106 . 10.1634/stemcells.2005-0479 . 25347875 . etal.