CNKSR1 explained

Connector enhancer of kinase suppressor of ras 1 is an enzyme that in humans is encoded by the CNKSR1 gene.^{[1] [2]}

Function

This gene is a necessary element in receptor tyrosine kinase pathways, possibly as a tyrosine phosphorylation target. It is involved in regulation of RAF in the MAPK pathway and may also play a role in a MAPK-independent pathway.

Interactions

CNKSR1 has been shown to interact with RhoD^[3] and RASSF1.^[4]

Further reading

• Maruyama K, Sugano S . Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides . Gene . 138 . 1–2 . 171–4 . 1994 . 8125298 . 10.1016/0378-1119(94)90802-8 .
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S . Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library . Gene . 200 . 1–2 . 149–56 . 1997 . 9373149 . 10.1016/S0378-1119(97)00411-3 .
• Therrien M, Wong AM, Kwan E, Rubin GM . Functional analysis of CNK in RAS signaling . Proc. Natl. Acad. Sci. U.S.A. . 96 . 23 . 13259–63 . 1999 . 10557308 . 23935 . 10.1073/pnas.96.23.13259 . 1999PNAS...9613259T . free .
• Hartsough MT, Morrison DK, Salerno M, Palmieri D, Ouatas T, Mair M, Patrick J, Steeg PS . Nm23-H1 metastasis suppressor phosphorylation of kinase suppressor of Ras via a histidine protein kinase pathway . J. Biol. Chem. . 277 . 35 . 32389–99 . 2002 . 12105213 . 10.1074/jbc.M203115200 . free .
• Matheny SA, Chen C, Kortum RL, Razidlo GL, Lewis RE, White MA . Ras regulates assembly of mitogenic signalling complexes through the effector protein IMP . Nature . 427 . 6971 . 256–60 . 2004 . 14724641 . 10.1038/nature02237 . 2004Natur.427..256M . 4416552 .
• Jaffe AB, Aspenström P, Hall A . Human CNK1 acts as a scaffold protein, linking Rho and Ras signal transduction pathways . Mol. Cell. Biol. . 24 . 4 . 1736–46 . 2004 . 14749388 . 344169 . 10.1128/MCB.24.4.1736-1746.2004 .
• Rabizadeh S, Xavier RJ, Ishiguro K, Bernabeortiz J, Lopez-Ilasaca M, Khokhlatchev A, Mollahan P, Pfeifer GP, Avruch J, Seed B . The scaffold protein CNK1 interacts with the tumor suppressor RASSF1A and augments RASSF1A-induced cell death . J. Biol. Chem. . 279 . 28 . 29247–54 . 2004 . 15075335 . 10.1074/jbc.M401699200 . free .
• Jaffe AB, Hall A, Schmidt A . Association of CNK1 with Rho guanine nucleotide exchange factors controls signaling specificity downstream of Rho . Curr. Biol. . 15 . 5 . 405–12 . 2005 . 15753034 . 10.1016/j.cub.2004.12.082 . 16479940 . free . 2005CBio...15..405J .
• Ziogas A, Moelling K, Radziwill G . CNK1 is a scaffold protein that regulates Src-mediated Raf-1 activation . J. Biol. Chem. . 280 . 25 . 24205–11 . 2005 . 15845549 . 10.1074/jbc.M413327200 . free .
• Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, Berriz GF, Gibbons FD, Dreze M, Ayivi-Guedehoussou N, Klitgord N, Simon C, Boxem M, Milstein S, Rosenberg J, Goldberg DS, Zhang LV, Wong SL, Franklin G, Li S, Albala JS, Lim J, Fraughton C, Llamosas E, Cevik S, Bex C, Lamesch P, Sikorski RS, Vandenhaute J, Zoghbi HY, Smolyar A, Bosak S, Sequerra R, Doucette-Stamm L, Cusick ME, Hill DE, Roth FP, Vidal M . Towards a proteome-scale map of the human protein-protein interaction network . Nature . 437 . 7062 . 1173–8 . 2005 . 16189514 . 10.1038/nature04209 . 2005Natur.437.1173R . 4427026 .
• Fritz RD, Radziwill G . The scaffold protein CNK1 interacts with the angiotensin II type 2 receptor . Biochem. Biophys. Res. Commun. . 338 . 4 . 1906–12 . 2006 . 16289034 . 10.1016/j.bbrc.2005.10.168 .
• Beausoleil SA, Villén J, Gerber SA, Rush J, Gygi SP . A probability-based approach for high-throughput protein phosphorylation analysis and site localization . Nat. Biotechnol. . 24 . 10 . 1285–92 . 2006 . 16964243 . 10.1038/nbt1240 . 14294292 .

Notes and References

1. Therrien M, Wong AM, Rubin GM . CNK, a RAF-binding multidomain protein required for RAS signaling . Cell . 95 . 3 . 343–53 . Dec 1998 . 9814705 . 10.1016/S0092-8674(00)81766-3 . 16587330 . free .
2. Web site: Entrez Gene: CNKSR1 connector enhancer of kinase suppressor of Ras 1.
3. Jaffe AB, Aspenström P, Hall A . Human CNK1 acts as a scaffold protein, linking Rho and Ras signal transduction pathways . Mol. Cell. Biol. . 24 . 4 . 1736–46 . Feb 2004 . 14749388 . 344169 . 10.1128/MCB.24.4.1736-1746.2004 .
4. Rabizadeh S, Xavier RJ, Ishiguro K, Bernabeortiz J, Lopez-Ilasaca M, Khokhlatchev A, Mollahan P, Pfeifer GP, Avruch J, Seed B . The scaffold protein CNK1 interacts with the tumor suppressor RASSF1A and augments RASSF1A-induced cell death . J. Biol. Chem. . 279 . 28 . 29247–54 . Jul 2004 . 15075335 . 10.1074/jbc.M401699200 . free .