BRD8 explained

Bromodomain-containing protein 8 is a protein that in humans is encoded by the BRD8 gene.^{[1] [2] [3]}

The protein encoded by this gene interacts with thyroid hormone receptor in a ligand-dependent manner and enhances thyroid hormone-dependent activation from thyroid response elements. This protein contains a bromodomain and is thought to be a nuclear receptor coactivator. Three alternatively spliced transcript variants that encode distinct isoforms have been identified.

Interactions

BRD8 has been shown to interact with Thyroid hormone receptor beta and Retinoid X receptor alpha.^[4]

Further reading

• Doyon Y, Côté J . The highly conserved and multifunctional NuA4 HAT complex . Current Opinion in Genetics & Development . 14 . 2 . 147–54 . April 2004 . 15196461 . 10.1016/j.gde.2004.02.009 .
• Maruyama K, Sugano S . Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides . Gene . 138 . 1–2 . 171–4 . January 1994 . 8125298 . 10.1016/0378-1119(94)90802-8 .
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S . Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library . Gene . 200 . 1–2 . 149–56 . October 1997 . 9373149 . 10.1016/S0378-1119(97)00411-3 .
• Monden T, Kishi M, Hosoya T, Satoh T, Wondisford FE, Hollenberg AN, Yamada M, Mori M . p120 acts as a specific coactivator for 9-cis-retinoic acid receptor (RXR) on peroxisome proliferator-activated receptor-gamma/RXR heterodimers . Molecular Endocrinology . 13 . 10 . 1695–703 . October 1999 . 10.1210/mend.13.10.0353 . 10517671 . 25098867 . free .
• Lai F, Godley LA, Joslin J, Fernald AA, Liu J, Espinosa R, Zhao N, Pamintuan L, Till BG, Larson RA, Qian Z, Le Beau MM . Transcript map and comparative analysis of the 1.5-Mb commonly deleted segment of human 5q31 in malignant myeloid diseases with a del(5q) . Genomics . 71 . 2 . 235–45 . January 2001 . 11161817 . 10.1006/geno.2000.6414 .
• Wiemann S, Weil B, Wellenreuther R, Gassenhuber J, Glassl S, Ansorge W, Böcher M, Blöcker H, Bauersachs S, Blum H, Lauber J, Düsterhöft A, Beyer A, Köhrer K, Strack N, Mewes HW, Ottenwälder B, Obermaier B, Tampe J, Heubner D, Wambutt R, Korn B, Klein M, Poustka A . Toward a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs . Genome Research . 11 . 3 . 422–35 . March 2001 . 11230166 . 311072 . 10.1101/gr.GR1547R .
• Cai Y, Jin J, Tomomori-Sato C, Sato S, Sorokina I, Parmely TJ, Conaway RC, Conaway JW . Identification of new subunits of the multiprotein mammalian TRRAP/TIP60-containing histone acetyltransferase complex . The Journal of Biological Chemistry . 278 . 44 . 42733–6 . October 2003 . 12963728 . 10.1074/jbc.C300389200 . free .
• Doyon Y, Selleck W, Lane WS, Tan S, Côté J . Structural and functional conservation of the NuA4 histone acetyltransferase complex from yeast to humans . Molecular and Cellular Biology . 24 . 5 . 1884–96 . March 2004 . 14966270 . 350560 . 10.1128/MCB.24.5.1884-1896.2004 .
• Beausoleil SA, Jedrychowski M, Schwartz D, Elias JE, Villén J, Li J, Cohn MA, Cantley LC, Gygi SP . Large-scale characterization of HeLa cell nuclear phosphoproteins . Proceedings of the National Academy of Sciences of the United States of America . 101 . 33 . 12130–5 . August 2004 . 15302935 . 514446 . 10.1073/pnas.0404720101 . 2004PNAS..10112130B . free .
• Cai Y, Jin J, Florens L, Swanson SK, Kusch T, Li B, Workman JL, Washburn MP, Conaway RC, Conaway JW . The mammalian YL1 protein is a shared subunit of the TRRAP/TIP60 histone acetyltransferase and SRCAP complexes . The Journal of Biological Chemistry . 280 . 14 . 13665–70 . April 2005 . 15647280 . 10.1074/jbc.M500001200 . free .
• Benevolenskaya EV, Murray HL, Branton P, Young RA, Kaelin WG . Binding of pRB to the PHD protein RBP2 promotes cellular differentiation . Molecular Cell . 18 . 6 . 623–35 . June 2005 . 15949438 . 10.1016/j.molcel.2005.05.012 . free .
• Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, Berriz GF, Gibbons FD, Dreze M, Ayivi-Guedehoussou N, Klitgord N, Simon C, Boxem M, Milstein S, Rosenberg J, Goldberg DS, Zhang LV, Wong SL, Franklin G, Li S, Albala JS, Lim J, Fraughton C, Llamosas E, Cevik S, Bex C, Lamesch P, Sikorski RS, Vandenhaute J, Zoghbi HY, Smolyar A, Bosak S, Sequerra R, Doucette-Stamm L, Cusick ME, Hill DE, Roth FP, Vidal M . Towards a proteome-scale map of the human protein-protein interaction network . Nature . 437 . 7062 . 1173–8 . October 2005 . 16189514 . 10.1038/nature04209 . 2005Natur.437.1173R . 4427026 .
• Natsume W, Tanabe K, Kon S, Yoshida N, Watanabe T, Torii T, Satake M . SMAP2, a novel ARF GTPase-activating protein, interacts with clathrin and clathrin assembly protein and functions on the AP-1-positive early endosome/trans-Golgi network . Molecular Biology of the Cell . 17 . 6 . 2592–603 . June 2006 . 16571680 . 1475504 . 10.1091/mbc.E05-10-0909 .
• Beausoleil SA, Villén J, Gerber SA, Rush J, Gygi SP . A probability-based approach for high-throughput protein phosphorylation analysis and site localization . Nature Biotechnology . 24 . 10 . 1285–92 . October 2006 . 16964243 . 10.1038/nbt1240 . 14294292 .
• Olsen JV, Blagoev B, Gnad F, Macek B, Kumar C, Mortensen P, Mann M . Global, in vivo, and site-specific phosphorylation dynamics in signaling networks . Cell . 127 . 3 . 635–48 . November 2006 . 17081983 . 10.1016/j.cell.2006.09.026 . 7827573 . free .

Notes and References

1. Nielsen MS, Petersen CM, Gliemann J, Madsen P . Cloning and sequencing of a human cDNA encoding a putative transcription factor containing a bromodomain . Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression . 1306 . 1 . 14–6 . April 1996 . 8611617 . 10.1016/0167-4781(95)00239-1 .
2. Monden T, Wondisford FE, Hollenberg AN . Isolation and characterization of a novel ligand-dependent thyroid hormone receptor-coactivating protein . The Journal of Biological Chemistry . 272 . 47 . 29834–41 . November 1997 . 9368056 . 10.1074/jbc.272.47.29834 . free .
3. Web site: Entrez Gene: BRD8 bromodomain containing 8.
4. Monden T, Kishi M, Hosoya T, Satoh T, Wondisford FE, Hollenberg AN, Yamada M, Mori M . p120 acts as a specific coactivator for 9-cis-retinoic acid receptor (RXR) on peroxisome proliferator-activated receptor-gamma/RXR heterodimers . Molecular Endocrinology . 13 . 10 . 1695–703 . October 1999 . 10.1210/mend.13.10.0353 . 10517671 . 25098867 . free .