| Ayn Issa Subdistrict | |
| Native Name: | ناحية عين عيسى |
| Native Name Lang: | ar |
| Type: | Subdistrict |
| Mapsize: | 250x250px |
| Map Alt: | Ayn Issa Subdistrict in Syria |
| Coor Pinpoint: | Ayn Issa |
| Pushpin Map: | Syria |
| Pushpin Label Position: | bottom |
| Pushpin Map Caption: | Location in Syria |
| Pushpin Mapsize: | 250 |
| Subdivision Type: | Country |
| Subdivision Type1: | Governorate |
| Subdivision Name1: | Raqqa |
| Subdivision Type2: | District |
| Parts Type: | Subdistricts |
| Parts Style: | para |
| Established Title: | Established |
| Seat Type: | Seat |
| Seat: | Ayn Issa |
| Elevation Point: | Ayn Isa |
| Population: | 40,912 |
| Population Density Km2: | auto |
| Population As Of: | 2004 |
| Area Code Type: | Area code |
| Geocode: | SY110202 |
Ayn Issa Subdistrict or Ayn Issa Nahiyah (ar|ناحية عين عيسى) is a Syrian Nahiyah (Subdistrict) located in Tell Abyad District in Raqqa. According to the Syria Central Bureau of Statistics (CBS), Ayn Issa Subdistrict had a population of 40,912 in the 2004 census.[1]