The women's 400 metres hurdles at the 2007 All-Africa Games were held on July 19–22.
| width=200 bgcolor=gold | Gold | width=200 bgcolor=silver | Silver | width=200 bgcolor=CC9966 | Bronze |
| Muna Jabir Adam | Aïssata Soulama | Joke Odumosu |
Qualification: First 3 of each heat (Q) and the next 2 fastest (q) qualified for the final.
| Rank | Heat | Name | Nationality | Time | Notes | |
|---|---|---|---|---|---|---|
| 1 | 2 | 56.65 | Q | |||
| 2 | 1 | 57.24 | Q | |||
| 3 | 2 | 57.32 | Q | |||
| 4 | 2 | 57.62 | Q | |||
| 5 | 1 | 57.90 | Q | |||
| 6 | 2 | 58.58 | q | |||
| 7 | 1 | 58.59 | Q | |||
| 8 | 1 | 58.80 | q | |||
| 9 | 2 | 58.97 | ||||
| 10 | 2 | 59.03 | ||||
| 11 | 1 |
| Rank | Name | Nationality | Time | Notes | |
|---|---|---|---|---|---|
| 54.93 | NR | ||||
| 55.49 | NR | ||||
| 55.80 | |||||
| 4 | 57.35 | ||||
| 5 | 57.37 | ||||
| 6 | 58.26 | ||||
| 7 | 58.98 | ||||
| 8 |