| Ana de Velasco y Téllez-Girón | |
| House: | Frías |
| Father: | Juan Fernández de Velasco, 5th Duke of Frías |
| Mother: | Maria Tellez-Giron |
| Spouse: | Teodósio II, Duke of Braganza |
| Issue: | John IV, King of Portugal Edward, Lord of Vila do Conde Catherine of Braganza Alexandre of Braganza |
| Birth Date: | 12 March 1585 |
| Birth Place: | Naples, Italy |
| Death Place: | Vila Viçosa |
Ana de Velasco y Téllez-Girón (1585 – 7 November 1607) was a Spanish noblewoman and mother of John IV of Portugal, the first Portuguese King of the Braganza Dynasty.
She was the daughter of Juan Fernández de Velasco, 5th Duke of Frías and María Tellez-Girón, daughter of Andalusian Duke of Osuna, Pedro Téllez-Girón, 1st Duke of Osuna
She married on 17 June 1603, Teodósio II, Duke of Braganza and had 4 children:
She died in Vila Viçosa at the age of 22.