| Date: | 17–22 June |
| Edition: | 5th |
| Surface: | Clay |
| Location: | Santa Cruz de la Sierra, Bolivia |
| Champs: |  Juan Manuel Cerúndolo |
| Champd: | Hady Habib /  Trey Hilderbrand |
| Before Name: | Santa Cruz Challenger |
| Before Year: | 2024 |
| Main Name: | Santa Cruz Challenger |
| After Name: | Santa Cruz Challenger |
The 2024 Santa Cruz Challenger II was a professional tennis tournament played on clay courts. It was the fifth edition of the tournament which was part of the 2024 ATP Challenger Tour. It took place in Santa Cruz de la Sierra, Bolivia between 17 and 22 June 2024.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
| Argentina | Juan Manuel Cerúndolo | 181 | 1 | |
 Bolivia, Plurination;al State of | Murkel Dellien | 214 | 2 | |
| Argentina | Andrea Collarini | 255 | 3 | |
| Hady Habib | 259 | 4 | ||
 Ecuador | Álvaro Guillén Meza | 280 | 5 | |
 Brazil | Pedro Sakamoto | 304 | 6 | |
| Argentina | Renzo Olivo | 305 | 7 | |
| Argentina | Facundo Mena | 307 | 8 |
The following players received wildcards into the singles main draw:
Agustín Eduardo Cuéllar Lorberg Raúl García Emilio GómezThe following player received entry into the singles main draw as a special exempt:
The following player received entry into the singles main draw using a protected ranking:
The following players received entry into the singles main draw as alternates:
Adrià Soriano Barrera Nicolas ZanellatoThe following players received entry from the qualifying draw:
Leonardo Aboian
Diego Augusto Barreto Sánchez Dali Blanch Guido Iván Justo José Pereira Noah SchachterThe following player received entry as a lucky loser:
See main article: 2024 Santa Cruz Challenger II – Singles.
Juan Manuel Cerúndolo def. Álvaro Guillén Meza 3–6, 6–1, 6–4.See main article: 2024 Santa Cruz Challenger II – Doubles.
Trey Hilderbrand def. 
Finn Reynolds / Matías Soto 3–6, 6–3, [10–7].