See main article: article and 2021 Prague Open.
Lucie Hradecká and Kristýna Plíšková were the defending champions, however Plíšková chose not to participate.
Hradecká played alongside Marie Bouzková and successfully defended her title, defeating Viktória Kužmová and Nina Stojanović in the final, 7–6(7–3), 6–4.[1]