| Champ: |  Garbiñe Muguruza |
| Runner: |  Barbora Krejčíková |
| Score: | 7–6(8–6), 6–3 |
| Draw: | 56 (8 / 3) |
| Seeds: | 16 |
See main article: 2021 Dubai Tennis Championships.
Garbiñe Muguruza defeated Barbora Krejčíková in the final, 7–6(8–6), 6–3 to win the women's singles tennis event at the 2021 Dubai Tennis Championships.[1] Muguruza dropped just one set during the tournament, to Aryna Sabalenka in the quarterfinals.
Simona Halep was the reigning champion,[2] but withdrew before the tournament due to an injury.[3]
The top eight seeds received a bye into the second round.
See also: 1 and 1. 
Elina Svitolina (second round)
See also: 2 and 4. Karolína Plíšková (third round)
See also: 3 and 3. 
Aryna Sabalenka (quarterfinals)
See also: 4 and 2. Petra Kvitová (second round, retired)
See also: 5 and 2. Kiki Bertens (second round)
See also: 6 and 1. Belinda Bencic (third round)
See also: 7. Victoria Azarenka (withdrew)
See also: 8 and 3. 
Iga Świątek (third round)
See also: 9 and 3. Garbiñe Muguruza (champion)
See also: 10 and 4. 
Elise Mertens (semifinals)
See also: 11 and 1. 
Madison Keys (second round)
See also: 12 and 2. Markéta Vondroušová (second round)
See also: 13 and 4. Petra Martić (first round)
See also: 14 and 2. 
Elena Rybakina (second round)
See also: 15 and 3. 
Anett Kontaveit (third round)
See also: 16 and 1. Maria Sakkari (first round)