The 2020 Ann Arbor Challenger was a professional tennis tournament played on indoor hard courts. It was the tournament's first edition, a part of the 2020 ATP Challenger Tour. It took place in Ann Arbor, Michigan, United States between January 6 and 12, 2020.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
| Bjorn Fratangelo | 207 | 1 | ||
| Noah Rubin | 210 | 2 | ||
| Sebastian Korda | 249 | 3 | ||
 Dominican Republic | Roberto Cid Subervi | 258 | 4 | |
 Belgium | Ruben Bemelmans | 260 | 5 | |
 Argentina | Renzo Olivo | 265 | 6 | |
 Ecuador | Roberto Quiroz | 266 | 7 | |
| JC Aragone | 275 | 8 | ||
| Nino Serdarušić | 278 | 9 | ||
| Daniel Altmaier | 279 | 10 | ||
 Japan | Kaichi Uchida | 286 | 11 | |
| Kevin King | 298 | 12 | ||
 Brazil | Pedro Sakamoto | 308 | 13 | |
| Michael Redlicki | 314 | 14 | ||
| Sekou Bangoura | 316 | 15 | ||
| Brazil | Thomaz Bellucci | 319 | 16 |
The following players received wildcards into the singles main draw:
Andrew Fenty Aleksandar Kovacevic John McNally
Ondřej Štyler Zachary SvajdaThe following players received entry into the singles main draw using protected rankings:
The following players received entry from the qualifying draw:
See main article: 2020 Ann Arbor Challenger – Singles.
Ulises Blanch def. Roberto Cid Subervi 3–6, 6–4, 6–2.See main article: 2020 Ann Arbor Challenger – Doubles.
Robert Galloway / 
Hans Hach Verdugo def. 
Nicolás Barrientos / Alejandro Gómez 4–6, 6–4, [10–8].