| Date: | 29 October – 3 November |
| Edition: | 14th |
| Surface: | Clay |
| Location: | Guayaquil, Ecuador |
| Champs: |  Guido Andreozzi |
| Champd: | Guillermo Durán /  Roberto Quiroz |
The 2018 Challenger Ciudad de Guayaquil was a professional tennis tournament played on clay courts. It was the fourteenth edition of the tournament which was part of the 2018 ATP Challenger Tour. It took place in Guayaquil, Ecuador between October 29 and November 3, 2018.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
| 66 | 1 | |||
| Argentina | ||||
| Argentina | Guido Andreozzi | 94 | 3 | |
 Italy | 114 | 5 | ||
 Brazil | Thiago Monteiro | 115 | 6 | |
 Bolivia, Plurination;al State of | Hugo Dellien | 116 | 7 | |
| Argentina | Juan Ignacio Londero | 122 | 8 |
The following players received wildcards into the singles main draw:
The following player received entry into the singles main draw using a protected ranking:
The following players received entry from the qualifying draw:
The following players received entry as lucky losers:
See main article: 2018 Challenger Ciudad de Guayaquil – Singles.
Guido Andreozzi def. Pedro Sousa 7–5, 1–6, 6–4.See main article: 2018 Challenger Ciudad de Guayaquil – Doubles.
Guillermo Durán / Roberto Quiroz def. Thiago Monteiro / Fabrício Neis 6–3, 6–2.
